Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F204 I0 R1 247 174.3 8556966 94.2% 8060661 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,971,690 A→C 100% Q144P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,971,6900AC98.9% 615.5 / ‑4.1 177Q144P (CAG→CCG) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (81/93);  total (83/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 182 total aligned reads displayed.

GGCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGT  >  NC_000913/3971593‑3971786
                                                                                                 |                                                                                                
    cgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCTACCGAGAAGATGGCAGAATGGTTAGCAGAGATGAGTCTGGAGCTCACTTTCCGGCCGCAGGt                                                                                            >  1:2283175/1‑100 (MQ=255)
    cgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTTCCGCCGCAGGt                                                                                            <  2:819110/100‑1 (MQ=255)
     gcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTg                                                                                           >  1:2952488/1‑100 (MQ=255)
          tGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGa                                                                                      <  1:998976/100‑1 (MQ=255)
            aTCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTGGCAGGGGTGCGTCCGGATCTCACTTTCCCGCGGCAGGTGGGCGAGc                                                                                    >  1:143590/1‑100 (MQ=255)
            aTCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAg                                                                                     <  1:2393376/99‑1 (MQ=255)
             tCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCa                                                                                   <  2:654651/100‑1 (MQ=255)
               cTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCCgg                                                                                 >  1:2072089/1‑100 (MQ=255)
               cTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAgg                                                                                 <  2:3182854/100‑1 (MQ=255)
               cTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGACGGCAGAATGGTTAGCAGAGATCCGTCCGCACCTCACTTTCCCGCCGCAgggggg                                                                                         >  1:1072872/1‑92 (MQ=255)
                ttGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGc                                                                                <  2:2241600/100‑1 (MQ=255)
                ttGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGc                                                                                <  1:3148843/100‑1 (MQ=255)
                 taaaTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGc                                                                                >  2:2035200/3‑99 (MQ=255)
                 tGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCg                                                                               <  1:259113/100‑1 (MQ=255)
                   aaTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGACTGGTTAGCAGGGATGCGCCCGGATCTCACTTTCCCGCCGCAGGGGGGCg                                                                                       >  1:3584749/1‑90 (MQ=255)
                   aaTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGa                                                                             <  1:3396211/100‑1 (MQ=255)
                   aaTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGa                                                                             <  1:62697/100‑1 (MQ=255)
                   aaTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGCCCGCATCTCACTTTCCCGCCGCAGGGGGGCGAGCAGGCGGa                                                                             >  1:4057025/1‑100 (MQ=255)
                    aTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGCATGGTTGGCAGAGATGCGGCCGGATCTCCCCTTACCGCCGCAGGTGGGCGAGCAGACGGAc                                                                            >  1:1071346/1‑100 (MQ=255)
                    aTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGAc                                                                            <  2:2931295/100‑1 (MQ=255)
                     tCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCGGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACg                                                                           >  1:554425/1‑100 (MQ=255)
                     tCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACg                                                                           <  1:2572376/100‑1 (MQ=255)
                     tCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACg                                                                           <  1:3035766/100‑1 (MQ=255)
                      ccACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACg                                                                           <  2:1525078/99‑1 (MQ=255)
                      ccACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGt                                                                          <  2:2929857/100‑1 (MQ=255)
                      ccACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGt                                                                          <  2:2562451/100‑1 (MQ=255)
                       cACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTc                                                                         >  2:512298/1‑100 (MQ=255)
                       cACCTCGCCGGTGGGGTCAACCGAGAAGATAGCAGAAAGGTTAGCAGAGATGCGGCCGGGTCTCACTTACACGCCGCAGGTGGGCCACCAGGAGGAcat                                                                          >  1:4214951/1‑97 (MQ=255)
                         ncTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCCGGCGGACGTCaa                                                                       >  1:2011702/2‑100 (MQ=255)
                         ccTCGCCGGTGGGGTCAACCGAGAAGATGGCAGCATGGTTAGCAGAGATGCGACCGGATCACAATCTCGCACCGCAGCTGGGCGAACAGGCGGACGTCa                                                                        >  1:4255002/1‑99 (MQ=255)
                         ccTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCaa                                                                       >  2:751463/1‑100 (MQ=255)
                          cTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAAc                                                                      >  2:1987331/1‑100 (MQ=255)
                          cTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAAGGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGTCGTCAAc                                                                      <  1:2240143/100‑1 (MQ=255)
                           tCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACa                                                                     <  2:213266/100‑1 (MQ=255)
                               cGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGc                                                                 <  2:3234179/100‑1 (MQ=255)
                               cGGTGGGGTCAACCAAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGc                                                                 <  2:120980/100‑1 (MQ=255)
                                ggTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCt                                                                >  1:2701485/1‑100 (MQ=255)
                                ggTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCt                                                                <  1:1636882/100‑1 (MQ=255)
                                 gTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCtt                                                               <  2:2706744/100‑1 (MQ=255)
                                   ggggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTAc                                                             <  2:2216704/100‑1 (MQ=255)
                                     ggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTg                                                           >  2:3980898/1‑100 (MQ=255)
                                        cAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGccc                                                        <  1:4160000/100‑1 (MQ=255)
                                        cAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGccc                                                        >  2:3525798/1‑100 (MQ=255)
                                        cAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGccc                                                        <  2:1370382/100‑1 (MQ=255)
                                        cAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGccc                                                        >  2:3649966/1‑100 (MQ=255)
                                            cGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCATGTGGGCGAGGAGGCGGACGTCAACATTGCTTACTGCCCGGaa                                                    >  1:3001767/1‑100 (MQ=255)
                                            cGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGaa                                                    >  2:928944/1‑100 (MQ=255)
                                            cGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGaa                                                    <  1:2200460/100‑1 (MQ=255)
                                              agaagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcg                                                  >  1:1710625/1‑100 (MQ=255)
                                                aagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                                <  1:394456/100‑1 (MQ=255)
                                                aagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                                <  1:470376/100‑1 (MQ=255)
                                                aagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                                <  2:3291369/100‑1 (MQ=255)
                                                  gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                              <  1:744489/100‑1 (MQ=255)
                                                  gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                              <  2:3858841/100‑1 (MQ=255)
                                                  gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                              <  1:3419122/100‑1 (MQ=255)
                                                   aTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtgt                                             <  2:3100781/100‑1 (MQ=255)
                                                   aTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtgt                                             <  1:3196545/100‑1 (MQ=255)
                                                    tGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGtt                                            <  2:1429762/100‑1 (MQ=255)
                                                    tGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGtt                                            <  2:3218160/100‑1 (MQ=255)
                                                    tGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGtt                                            <  1:3705058/100‑1 (MQ=255)
                                                       cAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTAcc                                         >  2:206607/1‑100 (MQ=255)
                                                        aGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGAACGTCAACATTGCTTACTGCCCGGGACGCGTGTTACCa                                        <  1:2631432/100‑1 (MQ=255)
                                                         gAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAg                                       <  2:342883/100‑1 (MQ=255)
                                                            tGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGAc                                    <  1:934784/100‑1 (MQ=255)
                                                              gTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAg                                  >  2:3157340/1‑100 (MQ=255)
                                                               ttAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAgg                                 <  1:1895276/100‑1 (MQ=255)
                                                                tAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGTCGAGCAGGCGGACGTCAACAGTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGt                                >  2:3001906/1‑100 (MQ=255)
                                                                tAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGt                                >  2:3072609/1‑100 (MQ=255)
                                                                  gCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTaa                              <  1:3964261/100‑1 (MQ=255)
                                                                    agagATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATg                            >  2:3175861/1‑100 (MQ=255)
                                                                       gaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTc                         >  1:1102926/1‑100 (MQ=255)
                                                                        aTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCg                        <  2:1877124/100‑1 (MQ=255)
                                                                         tGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGa                       >  1:4207739/1‑100 (MQ=255)
                                                                          gCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAg                      <  2:2249782/100‑1 (MQ=255)
                                                                           cGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGc                     <  2:2006413/100‑1 (MQ=255)
                                                                            gTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCt                    >  2:3283313/1‑100 (MQ=255)
                                                                            gTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCt                    >  1:3340006/1‑100 (MQ=255)
                                                                            gTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCt                    >  1:799811/1‑100 (MQ=255)
                                                                               cGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                 <  2:2613246/100‑1 (MQ=255)
                                                                               cGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                 <  1:3645755/100‑1 (MQ=255)
                                                                               cGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                 <  2:1954540/100‑1 (MQ=255)
                                                                               cGGATCTCACTTTCCCGCCGAAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                 <  1:2206973/100‑1 (MQ=255)
                                                                                    ctcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaaa            <  1:1633317/100‑1 (MQ=255)
                                                                                    ctcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaaa            >  2:448447/1‑100 (MQ=255)
                                                                                     tcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACCTTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaaaa           >  1:954434/1‑100 (MQ=255)
                                                                                     tcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaaaa           >  2:4119348/1‑100 (MQ=255)
                                                                                     tcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCGGCTTaaaaa           >  1:1535312/1‑100 (MQ=255)
                                                                                      cACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAAc          <  2:1096724/100‑1 (MQ=255)
                                                                                      cACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAAc          <  2:1096679/100‑1 (MQ=255)
                                                                                      cACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAGTaaaaa           >  1:4016259/1‑99 (MQ=255)
                                                                                       aCTTTCCCGCCGCAGGTGGGCGCGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAAc          >  1:267479/1‑99 (MQ=255)
                                                                                       aCTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACg         <  1:738914/100‑1 (MQ=255)
                                                                                        cTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGa        >  2:1548312/1‑100 (MQ=255)
                                                                                        cTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGa        >  2:3304789/1‑100 (MQ=255)
                                                                                           tCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcg     <  2:1659744/100‑1 (MQ=255)
                                                                                           tCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcg     >  2:560214/1‑100 (MQ=255)
                                                                                            cccGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcgc    >  2:4178105/1‑100 (MQ=255)
                                                                                            cccGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcgc    >  2:2449708/1‑100 (MQ=255)
                                                                                            cccGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcg     >  2:3961451/1‑99 (MQ=255)
                                                                                              cgccgcAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGt  >  1:4123496/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GGCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGT  >  NC_000913/3971593‑3971786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A