Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,403,176	C→T	P380L (CCG→CTG)	hflK →	modulator for HflB protease specific for phage lambda cII repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,403,176	0	C	T	100.0%	132.8 / NA	35	P380L (CCG→CTG)	hflK	modulator for HflB protease specific for phage lambda cII repressor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/13); total (22/13)

ATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATCGCG  >  NC_000913/4403028‑4403320

aTGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCtgctgc                                                                                                                                                >  2:139884/1‑151 (MQ=255)

  ggAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCa                                                                                                                                              <  1:707751/151‑1 (MQ=255)

     aaaaGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGcc                                                                                                                                           >  2:456065/1‑151 (MQ=255)

      aaaGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCct                                                                                                                                          >  2:503819/1‑151 (MQ=255)

                gTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCcac                                                                                                                                <  2:408127/151‑1 (MQ=255)

                gTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCcac                                                                                                                                <  1:612481/151‑1 (MQ=255)

                            aaGTCCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCTTTAGACCAGATGCTGAAAGGTGGTAATGCTCCTGCGGCGAAGAGCGATAACGTTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGagc                                                                                                                    <  1:509220/151‑1 (MQ=255)

                              gTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGagcaa                                                                                                                  <  1:215484/151‑1 (MQ=255)

                              gTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGagca                                                                                                                   >  1:721961/1‑150 (MQ=255)

                                     ttAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAacac                                                                                                           >  2:404906/1‑151 (MQ=255)

                                             aaaGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTcc                                                                                                    <  2:769622/150‑1 (MQ=255)

                                                   ggCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTc                                                                                             <  2:208611/151‑1 (MQ=255)

                                                           gATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGat                                                                                     <  1:278386/151‑1 (MQ=255)

                                                             tGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGatat                                                                                   >  1:555648/1‑151 (MQ=255)

                                                              ggTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAtatt                                                                                  >  1:279751/1‑151 (MQ=255)

                                                                ttCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAtattat                                                                                >  2:122554/1‑151 (MQ=255)

                                                                 tCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAtattat                                                                                <  2:102557/150‑1 (MQ=255)

                                                                             ccAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAAcgccgc                                                                   >  1:143534/1‑151 (MQ=255)

                                                                                         aGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAg                                                       <  1:415323/151‑1 (MQ=255)

                                                                                           gtggtAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGc                                                      >  1:746282/1‑150 (MQ=255)

                                                                                           gtggtAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCg                                                     >  1:636122/1‑151 (MQ=255)

                                                                                                aaCGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACg                                                >  1:359346/1‑151 (MQ=255)

                                                                                                  cGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGAc                                              >  2:367618/1‑151 (MQ=255)

                                                                                                   gCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACt                                             >  2:614327/1‑151 (MQ=255)

                                                                                                     cccTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTAc                                           <  1:143041/151‑1 (MQ=255)

                                                                                                        tGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTAcc                                          >  1:300702/1‑149 (MQ=255)

                                                                                                           ggcgAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGt                                     >  1:26975/1‑151 (MQ=255)

                                                                                                                    cGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGa                             >  2:450726/1‑150 (MQ=255)

                                                                                                                        aaCGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAAc                        >  2:557665/1‑151 (MQ=255)

                                                                                                                              gCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCg                  >  2:627458/1‑151 (MQ=255)

                                                                                                                                      tCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTc            >  2:178466/1‑149 (MQ=255)

                                                                                                                                         gctgcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGtt        >  1:542995/1‑150 (MQ=255)

                                                                                                                                            gcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATcg    <  1:467550/151‑1 (MQ=255)

                                                                                                                                            gcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATc     <  2:402229/150‑1 (MQ=255)

                                                                                                                                              gTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATcgcg  >  1:601138/1‑151 (MQ=255)

                                                                                                                                              gTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATcg    >  1:627081/1‑149 (MQ=255)

ATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATCGCG  >  NC_000913/4403028‑4403320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A