Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I1 R1 300 54.7 1718999 98.4% 1691495 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,403,176 C→T P380L (CCG→CTG)  hflK → modulator for HflB protease specific for phage lambda cII repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,403,1760CT100.0% 184.6 / NA 50P380L (CCG→CTG) hflKmodulator for HflB protease specific for phage lambda cII repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/22);  total (28/22)

TGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATCGC  >  NC_000913/4403029‑4403319
                                                                                                                                                   |                                                                                                                                               
tGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTgctgcc                                                                                                                                              >  2:59121/1‑151 (MQ=255)
   aaaaaGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGc                                                                                                                                           >  1:678874/1‑151 (MQ=255)
       aGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCctct                                                                                                                                       >  1:836992/1‑151 (MQ=255)
          gtTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTtc                                                                                                                                     <  2:301133/150‑1 (MQ=255)
                tAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCcac                                                                                                                               >  2:528820/1‑150 (MQ=255)
                 aaCACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCcaca                                                                                                                              >  1:326467/1‑150 (MQ=255)
                   cacCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAAcc                                                                                                                           >  2:435759/1‑151 (MQ=255)
                     cccGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAAcc                                                                                                                           >  1:740632/1‑149 (MQ=255)
                      ccGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAg                                                                                                                         >  2:141105/1‑150 (MQ=255)
                      ccGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGt                                                                                                                        >  1:169674/1‑151 (MQ=255)
                               gCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGagcaagc                                                                                                               <  1:284474/151‑1 (MQ=255)
                                cTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGagcaagc                                                                                                               >  2:2212/1‑150 (MQ=255)
                                     tAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACAcg                                                                                                         >  2:589272/1‑151 (MQ=255)
                                     tAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACAcg                                                                                                         >  1:273552/1‑151 (MQ=255)
                                       aCGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACAcgtc                                                                                                       <  2:651656/151‑1 (MQ=255)
                                           tAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGt                                                                                                                        <  2:762985/130‑1 (MQ=255)
                                           tAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGt                                                                                                                        >  1:762928/1‑130 (MQ=255)
                                            aaaGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTcca                                                                                                  <  2:840361/151‑1 (MQ=255)
                                                       ccTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAggg                                                                                        <  1:741096/150‑1 (MQ=255)
                                                         tGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCg                                                                                      >  1:629537/1‑150 (MQ=255)
                                                              gTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGAtatt                                                                                 >  1:443482/1‑150 (MQ=255)
                                                                  tGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATgg                                                                             >  2:733017/1‑150 (MQ=255)
                                                                  tGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATgg                                                                             >  2:187670/1‑150 (MQ=255)
                                                                      gTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTTCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCTCCAGTCAGGGCGATATTATGGACCaa                                                                        <  1:477561/151‑1 (MQ=255)
                                                                            ccAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAAcgccgc                                                                  <  2:796607/151‑1 (MQ=255)
                                                                             cAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCcgcg                                                                 >  2:67281/1‑151 (MQ=255)
                                                                                aTGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCa                                                              <  2:118663/151‑1 (MQ=255)
                                                                                         ggtggtAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGc                                                     <  2:723943/151‑1 (MQ=255)
                                                                                                    cccTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTAc                                          >  1:230807/1‑151 (MQ=255)
                                                                                                       tGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTAcc                                         >  2:64659/1‑149 (MQ=255)
                                                                                                        gcggcgAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAg                                       <  1:827712/150‑1 (MQ=255)
                                                                                                            cgAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCCACGCGCAGCGTAACGACTACCAGCGt                                    >  1:390738/1‑149 (MQ=255)
                                                                                                             gAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTc                                   >  2:304548/1‑149 (MQ=255)
                                                                                                             gAAGAGCCATAACGGTGCCAGCAGTCTGCTGCTTCTGCTGCCAGCCTCATCCTCCACAACCAGTGGAGCAAGCAACACATCGTACACCAGTCCGGGCGATATTAGGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAg                                 <  2:621465/151‑1 (MQ=255)
                                                                                                               agagCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAggg                               <  1:485988/151‑1 (MQ=255)
                                                                                                                  gCGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAggggg                             >  1:407836/1‑150 (MQ=255)
                                                                                                                   cGATAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGaa                           <  1:850917/151‑1 (MQ=255)
                                                                                                                     aTAACGGTGCCAGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATa                         <  2:237278/151‑1 (MQ=255)
                                                                                                                          gttGCCAGCAACCTGCTGCGCCTTCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGCCGTCCTCCAGTCAGGGCGATATTATGGAGCAACGCCGCGCCAACGCGCAGCGGAACGACTTCCAGCGTCAGGGGGAAGAACGa                     <  1:725112/148‑1 (MQ=255)
                                                                                                                                aGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTaa              <  1:222452/151‑1 (MQ=255)
                                                                                                                                aGCAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTa               >  2:654189/1‑150 (MQ=255)
                                                                                                                                  cAATCTGCTGCGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGt            >  2:950/1‑151 (MQ=255)
                                                                                                                                        gctgcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGGTGCGTAAGGCAGtt       >  1:35393/1‑150 (MQ=255)
                                                                                                                                        gctgcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAg         <  1:210805/148‑1 (MQ=255)
                                                                                                                                        gctgcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGtt       <  2:398046/150‑1 (MQ=255)
                                                                                                                                        gctgcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTa      <  2:230831/151‑1 (MQ=255)
                                                                                                                                        gctgcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAAAGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGtt       >  2:576495/1‑150 (MQ=255)
                                                                                                                                           gcGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATc    <  2:726576/150‑1 (MQ=255)
                                                                                                                                            cGTCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATcgc  >  2:402278/1‑151 (MQ=255)
                                                                                                                                              tCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCg                 <  2:711460/134‑1 (MQ=255)
                                                                                                                                              tCTGCTGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCg                 >  1:711417/1‑134 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATAAAGGTGGCAACCTGATGGTTCTGCCGTTAGACCAGATGCTGAAAGGTGGTAACGCCCCTGCGGCGAAGAGCGATAACGGTGCCAGCAATCTGCTGCGTCTGCCGCCAGCCTCTTCCTCCACAACCAGTGGAGCAAGCAACACGTCGTCCACCAGTCAGGGCGATATTATGGACCAACGCCGCGCCAACGCGCAGCGTAACGACTACCAGCGTCAGGGGGAATAACGATGCGTAAGTCAGTTATCGC  >  NC_000913/4403029‑4403319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A