Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,630,461	A→G	N151N (AAT→AAC)	ydfI ←	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,630,461	0	A	G	100.0%	157.6 / NA	45	N151N (AAT→AAC)	ydfI	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/21); total (24/21)

GCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTA  >  NC_000913/1630315‑1630600

gCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGtttt                                                                                                                                         <  2:117384/151‑1 (MQ=255)

gCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGtttt                                                                                                                                         <  1:365749/151‑1 (MQ=255)

      gAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGt                                                                                                                                   <  2:547960/151‑1 (MQ=255)

              aaCGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCt                                                                                                                           <  2:336972/151‑1 (MQ=255)

              aaCGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCt                                                                                                                           >  1:42008/1‑151 (MQ=255)

                       cATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCggg                                                                                                                  >  2:393365/1‑151 (MQ=255)

                       cATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCggg                                                                                                                  <  1:550775/151‑1 (MQ=255)

                            cATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATc                                                                                                             >  1:237581/1‑151 (MQ=255)

                               gACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCg                                                                                                            >  2:125235/1‑149 (MQ=255)

                                   gTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCAtt                                                                                                      <  1:283843/151‑1 (MQ=255)

                                      ttCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAAc                                                                                                   >  1:346339/1‑151 (MQ=255)

                                        cTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACt                                                                                                  >  1:247983/1‑150 (MQ=255)

                                            cATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCg                                                                                             <  1:301546/151‑1 (MQ=255)

                                               gttgtCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTGCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGt                                                                                           <  1:564658/150‑1 (MQ=255)

                                                     acatgacatgACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCgg                                                                                     >  1:300666/1‑150 (MQ=255)

                                                      catgacatgaCGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGGGGGGGTTTTGCACGCCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCgg                                                                                     >  2:253174/1‑149 (MQ=255)

                                                        tgacatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGgag                                                                                   >  2:339374/1‑149 (MQ=255)

                                                          acatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTg                                                                               >  2:538097/1‑151 (MQ=255)

                                                           catgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTgg                                                                              <  1:364129/151‑1 (MQ=255)

                                                            atgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTgg                                                                              >  2:29420/1‑150 (MQ=255)

                                                                  gTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATAcc                                                                       <  1:233659/151‑1 (MQ=255)

                                                                        gCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCtttt                                                                  <  1:584515/150‑1 (MQ=255)

                                                                         cGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTc                                                                >  2:350666/1‑151 (MQ=255)

                                                                          gggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCg                                                               <  2:264260/151‑1 (MQ=255)

                                                                            gAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGt                                                             >  1:557710/1‑151 (MQ=255)

                                                                                     ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCag                                                    <  2:192276/151‑1 (MQ=255)

                                                                                      cGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTc                                                      >  2:627172/1‑148 (MQ=255)

                                                                                      agCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGt                                                       >  1:607980/2‑147 (MQ=255)

                                                                                        cTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCag                                                    >  1:558959/1‑148 (MQ=255)

                                                                                            aCGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACa                                             <  2:346342/151‑1 (MQ=255)

                                                                                               gCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAAt                                           <  2:463959/150‑1 (MQ=255)

                                                                                                   gCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcgcg                                       >  1:602548/1‑150 (MQ=255)

                                                                                                    ccAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAt                                     <  2:642233/151‑1 (MQ=255)

                                                                                                     cAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAtt                                    >  2:88792/1‑151 (MQ=255)

                                                                                                       gcgcCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTg                                  <  1:578062/151‑1 (MQ=255)

                                                                                                             caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTc                            <  2:654851/151‑1 (MQ=255)

                                                                                                              aacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTc                            >  2:38445/1‑150 (MQ=255)

                                                                                                               acaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTcac                          <  2:620051/151‑1 (MQ=255)

                                                                                                                      ccccTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCg                    <  1:125232/150‑1 (MQ=255)

                                                                                                                      ccccTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGc                   >  1:599081/1‑151 (MQ=255)

                                                                                                                         cTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGc                >  1:605820/1‑151 (MQ=255)

                                                                                                                              gCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAac            >  2:132169/1‑150 (MQ=255)

                                                                                                                                tGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACg         >  1:484690/1‑151 (MQ=255)

                                                                                                                                tGTTTTCGGCTGGGGGGGGTTTTGCACGTCGGCGGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACg         >  1:62104/1‑151 (MQ=255)

                                                                                                                                        gCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGGATTAACTGTCCGGTCGCCGGAGAGTTGAAATACCCTTTTTCGGTGATTGTCAGATAGACAATCGCGATTTGCTGTTCCCACATCGCTGCCAACACGGTTTCTa  <  1:106660/150‑1 (MQ=255)

GCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTA  >  NC_000913/1630315‑1630600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A