Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,258,247 A→G F41L (TTC→CTC)  psuG ← pseudouridine 5'‑phosphate glycosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,258,2470AG100.0% 172.4 / NA 50F41L (TTC→CTC) psuGpseudouridine 5'‑phosphate glycosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/31);  total (19/31)

ATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATAT  >  NC_000913/2258101‑2258390
                                                                                                                                                  |                                                                                                                                               
aTGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCgg                                                                                                                                              <  1:279721/150‑1 (MQ=255)
         aCGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTga                                                                                                                                    <  2:191144/151‑1 (MQ=255)
           gACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTTCGAATAGTTTTTTCAACTTCTAATGCGGTCTGGGCATTTTGTGGGAGCGGGATTCCg                                                                                                                                       >  1:604993/1‑146 (MQ=255)
               cAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAaat                                                                                                                               >  1:215737/1‑150 (MQ=255)
                 gTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAaataat                                                                                                                            <  2:33493/151‑1 (MQ=255)
                   aaTTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATgg                                                                                                                          <  2:592969/151‑1 (MQ=255)
                      tCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTc                                                                                                                        <  1:132424/150‑1 (MQ=255)
                           ttcttcTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTc                                                                                                                   <  2:440545/150‑1 (MQ=255)
                             cttcttTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGGCTGGGCATTTTGTGGGAGCGGCATCCCGCGAGAAATAATGGTCGATTcc                                                                                                                  >  1:259602/1‑149 (MQ=255)
                               tcttTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCa                                                                                                                 <  1:211114/148‑1 (MQ=255)
                                   tGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCca                                                                                                           <  1:129691/150‑1 (MQ=255)
                                     cTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAACGCCCTGTTTACGAATAGGTTCTTCAACTTCAATTGCGGGCTGGGCATTTTGTGGGGGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCcaca                                                                                                         >  1:305757/1‑150 (MQ=255)
                                      ttAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACaa                                                                                                        >  2:289846/1‑150 (MQ=255)
                                       tAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAAc                                                                                                       <  1:337463/150‑1 (MQ=255)
                                                tttCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgtttttt                                                                                              >  2:452061/1‑150 (MQ=255)
                                                 ttCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttg                                                                                             <  1:542727/150‑1 (MQ=255)
                                                  tcatcaCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgt                                                                                            <  1:378433/150‑1 (MQ=255)
                                                  tcatcaCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgt                                                                                            <  1:433696/150‑1 (MQ=255)
                                                   catcaCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCAACCGgttttttgttt                                                                                          >  2:135178/1‑151 (MQ=255)
                                                        cgccgcCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTTTTCAACTTCAATTGCGGGCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCAACCGgttttttgtttttt                                                                                       >  1:31746/1‑149 (MQ=255)
                                                         gccgccAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTaaa                                                                                    <  2:31745/151‑1 (MQ=255)
                                                          ccgccAATAATGGCGATCGTGGCAGGTACAGCGCCCCGTTTACGAATAGTTTTTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgtttttt                                                                                       >  1:632833/1‑147 (MQ=255)
                                                           cgccAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTaaa                                                                                    <  1:432587/149‑1 (MQ=255)
                                                           cgccAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGc                                                                                  >  2:503773/1‑151 (MQ=255)
                                                                ataatGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCt                                                                             <  2:613452/151‑1 (MQ=255)
                                                                   atGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCa                                                                          <  1:539120/151‑1 (MQ=255)
                                                                    tGGCGATCGTGGCAGGTACAGCGCCCTGTTTTCGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCAACCGGGTTTTTGTTTTTTAAAGCGTCCTGCAc                                                                         >  1:388728/1‑151 (MQ=255)
                                                                         aTCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCg                                                                    <  1:527138/151‑1 (MQ=255)
                                                                           cGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCgg                                                                   <  1:413091/150‑1 (MQ=255)
                                                                                          gcCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTaataat                                                   <  2:51078/151‑1 (MQ=255)
                                                                                           cccTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAAtt                                                  <  1:327246/151‑1 (MQ=255)
                                                                                                tttACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAggg                                             >  2:652836/1‑151 (MQ=255)
                                                                                                 ttACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCGCTTCCGGGGAAATTTGTAATAATTCAggg                                             <  1:666380/150‑1 (MQ=255)
                                                                                                  tACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAgggg                                            >  1:460101/1‑150 (MQ=255)
                                                                                                      aaTAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAtt                                       >  1:310904/1‑151 (MQ=255)
                                                                                                       aTAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAttt                                      >  1:202828/1‑151 (MQ=255)
                                                                                                          gTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTa                                   <  2:202829/151‑1 (MQ=255)
                                                                                                             tcttcAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAAt                                 <  2:135323/150‑1 (MQ=255)
                                                                                                               ttcaacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTc                               >  2:114021/1‑150 (MQ=255)
                                                                                                                 caacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCTGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAg                             <  1:51626/150‑1 (MQ=255)
                                                                                                                  aacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGAc                           >  2:29313/1‑151 (MQ=255)
                                                                                                                      tcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACAtt                        <  1:289846/150‑1 (MQ=255)
                                                                                                                      tcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACAt                         <  2:496534/149‑1 (MQ=255)
                                                                                                                       caaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCt                      <  2:49349/151‑1 (MQ=255)
                                                                                                                               gTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTtctc              <  1:29314/151‑1 (MQ=255)
                                                                                                                                   gggCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCAc           <  2:477942/150‑1 (MQ=255)
                                                                                                                                         tttGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGat    >  1:486921/1‑151 (MQ=255)
                                                                                                                                          ttGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGat    >  1:205471/1‑150 (MQ=255)
                                                                                                                                           tgtgGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGata   <  1:212662/150‑1 (MQ=255)
                                                                                                                                            gtgGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCTCTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGatat  <  2:388732/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATAT  >  NC_000913/2258101‑2258390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A