Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,258,247	A→G	F41L (TTC→CTC)	psuG ←	pseudouridine 5'‑phosphate glycosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,258,247	0	A	G	97.4%	119.9 / ‑4.3	38	F41L (TTC→CTC)	psuG	pseudouridine 5'‑phosphate glycosidase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (15/22); total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

AATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATATAAC  >  NC_000913/2258120‑2258393

aaTTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATg                                                                                                                              <  1:141290/150‑1 (MQ=255)

   tCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTc                                                                                                                           <  1:93103/150‑1 (MQ=255)

     aaTTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCCACTTTAATTGCGGTCTGGGCATTTTGTTGGAGCGGCATCCCGTGAGAAATAATGGTc                                                                                                                           >  2:30299/1‑148 (MQ=255)

     aaTTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGGACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGGGAAATAATGGTCGAt                                                                                                                        >  2:406447/1‑151 (MQ=255)

      aTTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGGCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGAtt                                                                                                                       >  1:314088/1‑151 (MQ=255)

            tcttTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAg                                                                                                                   <  2:456715/149‑1 (MQ=255)

               ttGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGcc                                                                                                               <  1:223893/150‑1 (MQ=255)

                tGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCcac                                                                                                             >  1:601211/1‑151 (MQ=255)

                    tAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCCCCCCGTTTACGAATAAATTCTTCAACTTCAATTGCGGGCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAAcc                                                                                                         >  1:265349/1‑151 (MQ=255)

                     aaCCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCg                                                                                                        <  2:39422/151‑1 (MQ=255)

                                         gccAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTTTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCCGCGCCACAACCGGTTTTTTGTTTTTTAAAGCg                                                                                    >  2:540439/1‑151 (MQ=255)

                                          ccAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGt                                                                                   <  2:31439/151‑1 (MQ=255)

                                             ataatGGCGATCGTGGCAGGTACAGCGCCCCGTTTACGAATAGTTTCTTCAACCTCAATTGCGGTCTGGGCATTTTTTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTTGTTTTTAAAGCGTcc                                                                                 >  2:600576/1‑150 (MQ=255)

                                               aatGGCGATCGTGGCAGGTACAGCCCCCTGTTTACGAATAGTTTTTTCAACTTCAATTGCGGTCTGGGCCTTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGGGCCCCAACCGGTTTTTTTTTTTTTAAAGCGTcc                                                                                 >  2:188528/1‑148 (MQ=255)

                                                         gTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCgggg                                                                    <  2:331617/151‑1 (MQ=255)

                                                           ggCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGaa                                                                  <  1:388098/151‑1 (MQ=255)

                                                            gCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGaa                                                                  <  1:484996/150‑1 (MQ=255)

                                                              aGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAAtt                                                               <  2:410322/151‑1 (MQ=255)

                                                              aGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAAtt                                                               <  2:265342/151‑1 (MQ=255)

                                                                  aCAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGta                                                           <  1:131065/151‑1 (MQ=255)

                                                                  aCAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGt                                                            <  1:469333/150‑1 (MQ=255)

                                                                      cgcCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGtaataa                                                       <  1:131049/151‑1 (MQ=255)

                                                                            gTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCa                                                   <  1:542339/149‑1 (MQ=255)

                                                                                    aTAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAttt                                         >  2:355340/1‑151 (MQ=255)

                                                                                    aTAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAttt                                         >  1:309007/1‑151 (MQ=255)

                                                                                              caacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAg                                <  2:136171/150‑1 (MQ=255)

                                                                                               aacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGAc                              <  2:591397/151‑1 (MQ=255)

                                                                                                   tcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACAt                            <  1:525096/149‑1 (MQ=255)

                                                                                                    caaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCt                         <  1:518226/151‑1 (MQ=255)

                                                                                                      aTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTaa                                     >  1:5508/1‑137 (MQ=255)

                                                                                                      aTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTaa                                     <  2:5508/137‑1 (MQ=255)

                                                                                                      aTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGc                       >  2:220016/1‑151 (MQ=255)

                                                                                                             tCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTcc                >  1:556283/1‑151 (MQ=255)

                                                                                                                gggCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTcc                <  1:427593/148‑1 (MQ=255)

                                                                                                                    aTTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACg         >  1:4830/1‑151 (MQ=255)

                                                                                                                         gtgGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGAtata    <  2:421248/151‑1 (MQ=255)

                                                                                                                           gggAGCGACATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATATAAc  >  2:530028/1‑151 (MQ=255)

                                                                                                                           gggAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATATAAc  <  1:531008/151‑1 (MQ=255)

AATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATATAAC  >  NC_000913/2258120‑2258393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A