Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,812,547 6 bp→CGATGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,812,5470GC97.6% 662.8 / ‑3.5 288intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base C (149/131);  total (152/136)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,812,5480CG97.6% 663.7 / 2.0 288intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (149/131);  minor base A (2/5);  total (152/136)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,812,5490TA97.6% 658.3 / ‑4.6 288intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base A (149/131);  total (152/136)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,812,5500AT97.9% 672.1 / 7.2 288intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (2/0);  major base T (148/131);  minor base . (1/5);  total (152/136)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,812,5510CG99.3% 674.3 / NA 288intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (150/136);  total (152/136)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,812,5520TA99.7% 691.3 / ‑5.6 288intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (151/136);  minor base . (1/0);  total (152/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

TCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACGCTACTCTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATC  >  CP042583/1812273‑1812820
                                                                                                                                                                                                                                                                                    ||||||                                                                                                                                                                                                                                                                            
          cGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACtt                                                                                                                                                                                                                                                           >  1:1775213/1‑289 (MQ=255)
          cGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACtt                                                                                                                                                                                                                                                           >  1:2062560/1‑289 (MQ=255)
           gTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACtt                                                                                                                                                                                                                                                           <  2:1134958/288‑1 (MQ=255)
              gTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGt                                                                                                                                                                                                                                                       >  1:2607494/1‑289 (MQ=255)
                     aaggCGTAACGTTGAGATAAGCGGAAATCAGACCCTTGATCAAACCGATCACCGAACCAATGACACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGGTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAAc                                                                                                                                                                                                                                                  <  2:650032/285‑1 (MQ=255)
                       ggCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGAGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAAAAGaa                                                                                                                                                                                                                                              >  2:1403985/1‑288 (MQ=255)
                         cGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATACTAATACGCAACGAT‑GACGGGGTGAGAATATTACTTAAGTGAAACAGACtt                                                                                                                                                                                                                                            >  2:1815987/1‑289 (MQ=255)
                         cGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACtt                                                                                                                                                                                                                                            >  1:1400005/1‑289 (MQ=255)
                           tCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTaa                                                                                                                                                                                                                                          >  1:1065832/1‑289 (MQ=255)
                                             tAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCTCCTTGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGaa                                                                                                                                                                                                                        >  1:1605193/1‑289 (MQ=255)
                                             tAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGaa                                                                                                                                                                                                                        >  1:1437719/1‑289 (MQ=255)
                                                 cAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAAAACGC‑ACCGATGACTGGGTGAGAATTATACTTAAGTTAAAAAGACTTAAAACTTGTGGGTCCTGGAAaata                                                                                                                                                                                                                    >  2:1925164/1‑289 (MQ=255)
                                                           aTCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCa                                                                                                                                                                                                          >  2:2283486/1‑289 (MQ=255)
                                                             cAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGc                                                                                                                                                                                                        <  1:1389561/289‑1 (MQ=255)
                                                                    aTCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGAACATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGCATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTa                                                                                                                                                                                                 >  1:1099659/1‑289 (MQ=255)
                                                                     tCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACt                                                                                                                                                                                                                                                            >  1:720685/1‑229 (MQ=255)
                                                                                        gACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATAcc                                                                                                                                                                              >  2:1617591/1‑288 (MQ=255)
                                                                                          cAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGcc                                                                                                                                                                           <  1:1181305/289‑1 (MQ=255)
                                                                                          cAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGcc                                                                                                                                                                           <  1:2393393/289‑1 (MQ=255)
                                                                                           aaTCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGc                                                                                                                                                                            <  2:2452604/287‑1 (MQ=255)
                                                                                                      ccAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATTTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGgta                                                                                                                                                               >  2:1647973/1‑289 (MQ=255)
                                                                                                          cgcAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTTGGGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTgg                                                                                                                                                                                                                          <  1:2018142/227‑1 (MQ=255)
                                                                                                          cgcAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTTGGGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTgg                                                                                                                                                                                                                          >  2:2018142/1‑227 (MQ=255)
                                                                                                           gcAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATACTACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATAGCCAGCAAAACTAAAAAAACGACGAAATAAACGCCCACTTTCAGgtaagtaa                                                                                                                                                          >  2:501979/1‑289 (MQ=255)
                                                                                                           gcAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAac                                                                                                                                                                                          >  2:2622931/1‑257 (MQ=255)
                                                                                                                               ttcacggAACACTTTGTC‑GGGGTTTTCCATGGGCTGCAATAATGTCGCATGCACCACTGCCGCCAGGCCTTGCCGACCACCAGCAGAAAGATCGGTCCCCGGGGTGACAATTAAGCCTGCCACACCGAGAGCGATAATAATACGC‑ACCGATGACTGGGGGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGCAAAATAATAAGCGCAAGCAAAACTTAATGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAAc                                                                                                                                      <  2:2569563/284‑1 (MQ=255)
                                                                                                                                tCCGGGAACCATTTGTT‑GGCGTTATCCATGGACTGCAATAAGGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     <  1:2462129/289‑1 (MQ=255)
                                                                                                                                tCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     <  2:840641/289‑1 (MQ=255)
                                                                                                                                tCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     <  1:1288082/289‑1 (MQ=255)
                                                                                                                                tCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     <  1:2281892/289‑1 (MQ=255)
                                                                                                                                tCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGACGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     <  2:2607494/289‑1 (MQ=255)
                                                                                                                                  cGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCa                                                                                                                                   <  2:2674692/289‑1 (MQ=255)
                                                                                                                                           tttGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAac                                                                                                                                                                                          <  1:1516274/225‑1 (MQ=255)
                                                                                                                                           tttGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAac                                                                                                                                                                                          >  2:1516274/1‑225 (MQ=255)
                                                                                                                                           tttGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGa                                                                                                                          <  1:2556338/289‑1 (MQ=255)
                                                                                                                                           tttGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGa                                                                                                                          <  1:1819502/289‑1 (MQ=255)
                                                                                                                                           tttGTG‑GGAGTTAGCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAAGACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGa                                                                                                                          <  2:2430215/289‑1 (MQ=255)
                                                                                                                                             tgtt‑gGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAg                                                                                                                        <  2:1838638/289‑1 (MQ=255)
                                                                                                                                                t‑gGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGccc                                                                                                                     <  1:2343708/289‑1 (MQ=255)
                                                                                                                                                    cGTTAGCCATGGACTGCGATAAGGTCGCAGCACCCCCTGCCGCCAGCCCTACCCGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCt                                                                                                                   <  2:1183964/288‑1 (MQ=255)
                                                                                                                                                              ggACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     >  2:471701/1‑260 (MQ=255)
                                                                                                                                                              ggACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     <  1:471701/260‑1 (MQ=255)
                                                                                                                                                              ggACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGa                                                                                                                          <  1:706544/271‑1 (MQ=255)
                                                                                                                                                                  tGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATAc                                                                                                                                                                               <  1:310024/214‑1 (MQ=255)
                                                                                                                                                                  tGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     >  2:573231/1‑256 (MQ=255)
                                                                                                                                                                   gCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACCGACTTAAAACTGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAAAACGACGTAAATACCGCCCTCTTTCAGCTAAGTAAGAAAACTTTTCTTATTTAACCCACTCATGGAAAGCCCTTAATCTTaaggttacaa                                                                                                   >  2:797890/1‑281 (MQ=255)
                                                                                                                                                                   gCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAAc                                                                                                                                                                                                   <  1:825253/193‑1 (MQ=255)
                                                                                                                                                                    cAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAac                                                                                                                                                                                          <  1:868598/201‑1 (MQ=255)
                                                                                                                                                                    cAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAac                                                                                                                                                                                          >  2:868598/1‑201 (MQ=255)
                                                                                                                                                                    cAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGccc                                                                                                                     >  2:2653362/1‑270 (MQ=255)
                                                                                                                                                                    cAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGccc                                                                                                                     <  1:2653362/270‑1 (MQ=255)
                                                                                                                                                                    cAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAAt                                                                                                                >  1:61970/1‑275 (MQ=255)
                                                                                                                                                                    cAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCaaa                                                                                                  >  1:1033039/1‑289 (MQ=255)
                                                                                                                                                                          tGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAaagacgca                                                                                            >  2:1003266/1‑289 (MQ=255)
                                                                                                                                                                            tCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAac                                                                                                                                                                                          <  1:511759/193‑1 (MQ=255)
                                                                                                                                                                            tCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGc                                                                                                                                                                            >  1:352263/1‑207 (MQ=255)
                                                                                                                                                                            tCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAaagacgcaag                                                                                          >  1:784045/1‑289 (MQ=255)
                                                                                                                                                                            tCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAaagacgcaag                                                                                          >  2:2104013/1‑289 (MQ=255)
                                                                                                                                                                              gCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCaaa                                                                                                                                                                                                     >  1:1877117/1‑180 (MQ=255)
                                                                                                                                                                              gCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCaaa                                                                                                                                                                                                     <  2:1877117/180‑1 (MQ=255)
                                                                                                                                                                               cAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGcc                                                                                                                                                                           <  2:1722094/205‑1 (MQ=255)
                                                                                                                                                                               cAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGcc                                                                                                                                                                           >  1:1722094/1‑205 (MQ=255)
                                                                                                                                                                               cAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAaagacgcaagac                                                                                        >  2:375868/1‑288 (MQ=255)
                                                                                                                                                                                      cacTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGc                                                                                                                                                                            >  1:851685/1‑197 (MQ=255)
                                                                                                                                                                                      cacTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAAtt                                                                                <  2:1359531/289‑1 (MQ=255)
                                                                                                                                                                                                aGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGACTTTCGTTTTGCg                                                                      >  1:2520215/1‑289 (MQ=255)
                                                                                                                                                                                                aGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCg                                                                      >  2:2181794/1‑289 (MQ=255)
                                                                                                                                                                                                    cTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgt                                                                  <  1:1624655/289‑1 (MQ=255)
                                                                                                                                                                                                       ccTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAACACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgt                                                               >  2:748357/1‑289 (MQ=255)
                                                                                                                                                                                                            cgacCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAGTAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCt                                                                                                                   <  2:300679/232‑1 (MQ=255)
                                                                                                                                                                                                            cgacCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATATCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCa                                                                                                                                   >  2:349289/1‑216 (MQ=255)
                                                                                                                                                                                                                 agcagAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCgttgttgtt                                                              <  1:1984906/280‑1 (MQ=255)
                                                                                                                                                                                                                 agcagAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCg                                                     >  1:226613/1‑289 (MQ=255)
                                                                                                                                                                                                                             ggTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGa                                                                                                                          <  1:159279/208‑1 (MQ=255)
                                                                                                                                                                                                                             ggTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGaa                                         >  1:1935847/1‑289 (MQ=255)
                                                                                                                                                                                                                                    tGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGCCGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgaga                                   >  2:2541715/1‑288 (MQ=255)
                                                                                                                                                                                                                                    tGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgaga                                   >  1:12331/1‑288 (MQ=255)
                                                                                                                                                                                                                                    tGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACgaga                                   >  1:274417/1‑288 (MQ=255)
                                                                                                                                                                                                                                         gACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGtt                                                                                                                                                                                                                                   <  1:398833/91‑1 (MQ=255)
                                                                                                                                                                                                                                            aaTTACCCCGGCCATACCGAGCGCTATAATAAGACCC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATTTTTGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGGAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTGAAAGGTGCAAAGACTCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTc                          <  2:1099659/289‑1 (MQ=255)
                                                                                                                                                                                                                                            aaTTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTAAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTc                          <  1:2620506/289‑1 (MQ=255)
                                                                                                                                                                                                                                                aaCCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGGTTTTGTATCGACAATTCCGGAAACGAGAACATTGcccttta                      >  2:1743232/1‑283 (MQ=255)
                                                                                                                                                                                                                                                 aCCCTGCCACACCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGa                      >  2:93437/1‑288 (MQ=255)
                                                                                                                                                                                                                                                        cacaCCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTaa                                                                                                                                       >  1:2492208/1‑168 (MQ=255)
                                                                                                                                                                                                                                                        cacaCCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTaa                                                                                                                                       <  2:2492208/168‑1 (MQ=255)
                                                                                                                                                                                                                                                         acacCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATAc             <  2:1295589/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          cacCGAGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACg            <  1:563980/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     <  2:2693581/163‑1 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACg                                                                                                                                     >  1:948391/1‑163 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGa                                                                                                                          <  1:2676860/174‑1 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATc                                                      <  1:1115872/242‑1 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCt                           <  1:2359864/269‑1 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCt                           >  2:2359864/1‑269 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATTACCAGAATACgg           >  2:1486468/1‑285 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACgg           >  2:492484/1‑285 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACg            <  1:2608033/284‑1 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACg            >  2:1347740/1‑284 (MQ=255)
                                                                                                                                                                                                                                                               aGCGCGATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACg            <  1:1347740/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gcgATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAGGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGtt     <  2:1594552/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gcgATAATAATACGC‑ACCGATGACTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTa    <  1:2448275/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    ||||||                                                                                                                                                                                                                                                                            
TCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCATTTCCGGGAACACTTTGTT‑GGCGTTATCCATGGACTGCAATAATGTCGCAGCCACCACTGCCGCCAGCCCTACCTGACGACCAGCAGAAAGATCGGTCCCCTGGGTGACAATTAACCCTGCCACACCGAGCGCGATAATAATACGC‑ACGCTACTCTGGGTGAGAATATTACTTAAGTTCAACAGACTTAAAAATGTTGGGTCCTGGAAAATAATAATCGCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATC  >  CP042583/1812273‑1812820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A