Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F111 I1 R1	16	82.1	2903858	91.9%	2668645	142.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	98.4%	184.3 / NA	62	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (32/29); minor base G (0/1); total (32/30)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132650‑2132925

tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGACATGCCCGGATAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                       <  1:205373/143‑1 (MQ=255)

    gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                   >  1:1193075/1‑143 (MQ=255)

     tAGAGGGTAAACAGCTGATATTTTTTGCTGACGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAGGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCGc                                                                                                                                  >  2:1176564/1‑143 (MQ=255)

      agagGGTAAACAGCTGATATTTTTGGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                                 <  1:220503/143‑1 (MQ=255)

          gggaaaCAGCTGATATTGTGTGCTCGCGCGGTCGCAGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                             <  1:1122429/140‑1 (MQ=255)

            tagatatcTGATATTTTTGGCTGGGGCGGTCGGATTTGTTTTTGTGCTGTGGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct                                                                                                                           <  2:993304/136‑1 (MQ=255)

                 tgCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAGACGTGGTCGTTCCCGGTGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGCTCTATATGCAGGCTCTGCGTCGCCTCTTCTACttt                                                                                                                      <  2:26492/142‑1 (MQ=255)

                  gCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                     >  1:631721/1‑143 (MQ=255)

                    tGATATTTTTTGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAGGTGGAAGTACCAGGAGCCTTTTATCGCGAACCGAGTGTTGTGGAGGTCATCGTGATTGATATGCCGGCCCCTCGTCGCCTCTTCTACTCTCCa                                                                                                                   >  2:1409943/1‑143 (MQ=255)

                    tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTCTCCa                                                                                                                   >  2:808829/1‑143 (MQ=255)

                     gATATTTTTTGCTGGCGCGGTCGGATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc                                                                                                                  >  1:1182241/1‑143 (MQ=255)

                     gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc                                                                                                                  <  1:693676/143‑1 (MQ=255)

                         ttttttGCTGGCGCGGTTGAATTTGTCTTTGTTATTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                              <  2:1099027/143‑1 (MQ=255)

                              tGCGGGCGCGGTCGACGTGGTCGTTGTGCTTGCGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCTCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                         <  2:171534/143‑1 (MQ=255)

                                cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                       >  2:257404/1‑143 (MQ=255)

                                 tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                      <  2:1049458/143‑1 (MQ=255)

                                   gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                                    <  2:406894/143‑1 (MQ=255)

                                       ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                                <  1:598505/143‑1 (MQ=255)

                                         tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac                                                                                              >  1:1390356/1‑143 (MQ=255)

                                           gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAGTGTGATGGAAAAGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacca                                                                                            >  1:1057105/1‑143 (MQ=255)

                                           gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTCCTccaccccca                                                                                            >  2:907368/1‑143 (MQ=255)

                                           gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccccca                                                                                            >  1:157384/1‑143 (MQ=255)

                                                   cTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAgg                                                                                    >  1:1066617/1‑143 (MQ=255)

                                                        tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGCAGAt                                                                               >  2:452186/1‑143 (MQ=255)

                                                         gCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                              <  1:1068036/143‑1 (MQ=255)

                                                           ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                            <  1:121700/143‑1 (MQ=255)

                                                              cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTa                                                                         >  1:1447431/1‑143 (MQ=255)

                                                               ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACCCCCCCACCATCCGCCGGAAGCTGTCGTaa                                                                        >  2:108507/1‑143 (MQ=255)

                                                                gtagaggTGGAAGTACCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt                                                                       <  2:626921/137‑1 (MQ=255)

                                                                gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCCGGACGCTGTCGGAAt                                                                       >  1:1325805/1‑143 (MQ=255)

                                                                 tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAACGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                      >  1:757065/1‑143 (MQ=255)

                                                                  aaaaGTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc                                                                     <  1:1440927/143‑1 (MQ=255)

                                                                    aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtg                                                                   <  1:334140/143‑1 (MQ=255)

                                                                     tgttgatgTACCCGGTCAATTTTTTCGGTGACGAGGTGATCTGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                  <  1:751655/136‑1 (MQ=255)

                                                                     aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAACACGTCGTAATCCtgt                                                                  >  2:1076188/1‑143 (MQ=255)

                                                                     aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGACGTCGTAATCCtgt                                                                  >  2:1280360/1‑143 (MQ=255)

                                                                      gTGGAAGTCCCCGGAGAAGTGTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                 <  2:1131056/143‑1 (MQ=255)

                                                                        ggAAGTACCCGGAGAATTTTTTCGGTGAGGAGGTCCTCTGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCCTCTACTTTACACGGTCCGCCGTACTCCACCACCCTGCGCAGGAACACGTCCTAATGCTGTGcc                                                               >  2:106626/1‑143 (MQ=255)

                                                                             tgcccGGAGAATTTGTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                          <  2:111246/141‑1 (MQ=255)

                                                                             tACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                          <  2:1193075/143‑1 (MQ=255)

                                                                             tACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                          <  1:842324/143‑1 (MQ=255)

                                                                                ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTACACGTTTCTCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTACCCCCATGCCGCTTTCAg                                                       >  2:506242/1‑143 (MQ=255)

                                                                                        ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACGACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                               >  1:247504/1‑143 (MQ=255)

                                                                                          ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTccgcaacc                                             >  2:481169/1‑136 (MQ=255)

                                                                                          tttaCGGTGACGGGCTGATCTGCATTTCACCATGATTGACATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAAGGGTTCGCCGTACTCCGCCACCGTCCTCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                             <  1:1268525/143‑1 (MQ=255)

                                                                                                ctgtcttggTGTTCTGCATTTCTCCGTGGTTGTTCTGCACGGTCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCTACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                       <  2:68230/136‑1 (MQ=255)

                                                                                                        gTGATCTGTGTTTCCCCGTGATTGATATGCAGGCTCTCCGTCGCCTCGTCTACTTTCCTCGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACTCTCttt                               <  2:1112947/143‑1 (MQ=255)

                                                                                                        gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCATTGCCGCTTTCAGTTCGGTATCGGacacacacccctt                               >  2:832505/1‑138 (MQ=255)

                                                                                                          gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                             <  1:49865/143‑1 (MQ=255)

                                                                                                           aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                            >  1:1014007/1‑143 (MQ=255)

                                                                                                           aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                            <  1:920613/143‑1 (MQ=255)

                                                                                                             cTGCATTTCACCGTGAGTGATATGCAGGCTCTGCGTCGACTCTTCTACTTTCCACGGTTAGCCGTACTCCACCAACAGCCGCAGGAAGGTGTCGTAATCGTGTGCAGCTTTCGGTTCGGTGTAGAACAAACACGCGTTGaaga                          >  1:364628/1‑141 (MQ=255)

                                                                                                                  tctacccGTGATTGATAGGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt                     <  1:1433781/138‑1 (MQ=255)

                                                                                                                   ttcgctgTGATTTATATGCAGGCTCTGCGTCGCGTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATg                    <  2:737576/137‑1 (MQ=255)

                                                                                                                    tCACCGTGATTGATATGCAGGCCCTGCGTCGCATCCCCTACTTGCCGCGTCCCCCCGCCCTCCACCACCATCCGCACGACGCTGTCGTACTCCTGTGCCCCCTTCCGTCCGGGACCGACCAACCACTCTTTGAGACGCCaccc                   >  2:1033402/1‑140 (MQ=255)

                                                                                                                       ccGTGATTGGTTGGCAGGCCCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa                <  2:631721/143‑1 (MQ=255)

                                                                                                                        cGTGATTGATATGCAGGCTCTGCGTCGTCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGGCCAg               >  2:384565/1‑143 (MQ=255)

                                                                                                                             tgtagatgcatgcTCTGCGCCGCCGCTCCTACTTTCCACGGTTCGCCGCATTCCTCCATCGTCCGCAGGAAGATGGCGTAATCCTGAGCCGCTCTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa          <  2:1252623/138‑1 (MQ=255)

                                                                                                                              tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAAGGGTAGTAATCCTGTGCCGCCTTCAGTACGATCTCGAACCAACAACATTTGAAAGGCCAATCACAGGGAAAg         >  2:1176723/1‑143 (MQ=255)

                                                                                                                                atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTTCTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc       <  1:1434947/143‑1 (MQ=255)

                                                                                                                                atatGCAGGCTCTGCGACACCTATTCGGCTATCCACGGTTAGCCGTACTCCACCACCATCCGCAGGAAGATGGCGTAATCCTGTGCCGCTTTCAGTTCGGCCTCGCACAAACCCTCTTTGAAACGCCATGCCCCGGTAAAGAc       >  2:883691/1‑143 (MQ=255)

                                                                                                                                  atGCAGGCTCTGCGTCGACTCTTATACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATACTGTGCCGCTTTCAGATCGGTATCGCACAGGCACGCGTTGAAACGCCAAGCTCAGGTAGACACCt     >  2:769465/1‑143 (MQ=255)

                                                                                                                                     cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGGCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAGGCCCGGGTAAAGACCGGGt  >  1:38519/1‑143 (MQ=255)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132650‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A