Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F113 I2 R1	19	51.3	1830242	91.7%	1678331	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	102.3 / NA	38	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (25/13); total (25/13)

GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132648‑2132924

gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGGGCTTGCGGTAAAAGTGGAAGTACCCGGAGAGTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATATGCAGGctct                                                                                                                                        >  1:454431/1‑143 (MQ=255)

        agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACTAGGTCATCTGCATTTCACCTTGATTGATATGCAAGCTCTGCGTCTcc                                                                                                                                >  1:651526/1‑143 (MQ=255)

               aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                         >  1:470134/1‑143 (MQ=255)

                 aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCAGGCCCTGCGTCGCCCCTTCTCCt                                                                                                                       >  1:837419/1‑143 (MQ=255)

                   aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt                                                                                                                     >  1:101906/1‑143 (MQ=255)

                                tggttgcgtGGTCGGATTTGCTGTTGTGCTGTCGTTGAGAGTGGAAGTGGCCGGAGGATTTGTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                        <  2:244597/138‑1 (MQ=255)

                                 gCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATCTGCAGGCTCTGCGGCGCCTCTTCTACTTTCCACGCGTCGCCgc                                                                                                       >  2:503774/1‑142 (MQ=255)

                                   tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGCAc                                                                                                     >  2:481367/1‑143 (MQ=255)

                                   tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGGGATCTGCATTTCACCGTGATTCATATGCAGGCGCTGCGTCGCCTCTCCTACCTTCAACGGTCCCCCGTAc                                                                                                     >  2:294799/1‑143 (MQ=255)

                                   tGGCGCGGTCGAATTTGTATTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGGATTGATATGCAGGCCCTGCGGCGCCTCTTATCATTTCCACGCTTAGCCGtcc                                                                                                     >  2:584090/1‑141 (MQ=255)

                                     gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGCTCGCCGTACTc                                                                                                   >  2:801454/1‑143 (MQ=255)

                                     gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAACGGTTCGCCGTCCTc                                                                                                   >  1:833191/1‑143 (MQ=255)

                                         ggTTGAATTTGTCTTAGTGCTTGCGGTAAAAGTGGAAGGACCCGGAGAATGTTTTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                               <  1:691644/143‑1 (MQ=255)

                                           tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCAGTTCCGCATACTccaccac                                                                                             >  1:563999/1‑143 (MQ=255)

                                            cGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc                                                                                            <  1:11983/143‑1 (MQ=255)

                                               aTTTGGCTTTGTGCTTGCGGTAAACGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc                                                                                         <  1:115498/143‑1 (MQ=255)

                                                  tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc                                                                                      <  1:626307/143‑1 (MQ=255)

                                                    tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATCCGCAg                                                                                    >  2:242776/1‑143 (MQ=255)

                                                           gCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAACGGTTCGCCGTACCCCACCACCATCCGCAGGACGATg                                                                             >  2:481810/1‑143 (MQ=255)

                                                                cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCgct                                                                        >  1:457275/1‑141 (MQ=255)

                                                                     aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGCTTCGCCGTACTCCACCACCATCCGCCGGACGCTGTCCTCATCCt                                                                   >  2:270719/1‑143 (MQ=255)

                                                                       aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCCCCGGACGATGTCGTAATCCtgt                                                                 >  2:607753/1‑143 (MQ=255)

                                                                           gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTGGCCGTACTCAACCACCATCCGCAGGAAGAAGTAGTAGTGCTGTGCCg                                                             >  2:485071/1‑143 (MQ=255)

                                                                               tACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTGGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATGCGGAGGAAGATGTGGTAATCGTGTGCCGCgtt                                                         >  1:260417/1‑143 (MQ=255)

                                                                                 cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCCACGGTTCGGCGTACTCCACCACCATCCGCAGGGAGATGTCGTCATAATGAGCCGCCTTCa                                                       >  2:722651/1‑143 (MQ=255)

                                                                                  ccGGAGGATTTTTTCGGTGACGATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                      <  1:76062/143‑1 (MQ=255)

                                                                                  ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTACACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTcgg                                                      >  1:459185/1‑141 (MQ=255)

                                                                                       gcatgttttCGGTGACGGGGTGATCTGCACTTCACCGTGATTGACATGCAGCCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                 <  2:837419/138‑1 (MQ=255)

                                                                                        agctttttCGGTGAGTGGGGGTTCGGCGTTGCACCGTGTTTGATCTGCAGGCTCTGCGTCGCCTCGTCGACTTTCCACGGTTCGCCGTACGCCACCACCCTCCGCAGGAAGATGTCGTAATCCGGTGCCGCTTTCAGTTCGGt                                                <  2:685141/140‑1 (MQ=255)

                                                                                                    gACGAGGTGATCTGCATTTCACCGTGTTTGATATGCAGGCTCTGCGTCGCCTATACACGTATCCACAGTTCTCCGTACTCCCCCACCACCCGCAGGAAGATGTCGTAATCCTGTACCGCCTTCAGTTCGGTATCGaacacccc                                    >  2:648387/1‑139 (MQ=255)

                                                                                                         ggTGATCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCGTTCAGTTCGGTATCGAACAAACACTCtt                               <  2:606518/143‑1 (MQ=255)

                                                                                                               cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc                         <  2:235933/143‑1 (MQ=255)

                                                                                                                tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGACGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCTTTCCGTGCGGTATCGAACCAACACTCTTTGCAACg                        >  1:79697/1‑143 (MQ=255)

                                                                                                                 gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATACTGTCGTTCTTTCAGTTCGGTATCGAACAAACACTCTTTGACACGc                       >  1:759298/1‑143 (MQ=255)

                                                                                                                    tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt                    <  1:528667/143‑1 (MQ=255)

                                                                                                                    cttCACGGTGATTGGTATGCAGTCTCGGCGCCGCCTCGTCTACTTTCCACGGTCCGCGGTACTCCACCCCCTGCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCAGGTCGGTATCGAACAAACACTCTTTGAAACGCCAt                    <  2:440018/142‑1 (MQ=255)

                                                                                                                            tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGCAACGCCATGCCCCGGt            >  1:325158/1‑143 (MQ=255)

                                                                                                                                     cgCAGGCTGTGCGTCGCTTCTTCTACGTTCGGCGGTTCGCCGTACTCCACCACCTTCAGTAGGAAGATTTCGTAAGCCCGTGCCGGCTTCAATTCGGTATCGAACAAACACTCTTTGAAACGCCATGGCCAGGTAAAGACCTg   <  2:866646/142‑1 (MQ=255)

                                                                                                                                      gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg  >  1:555666/1‑143 (MQ=255)

GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132648‑2132924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A