Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F3 I0 R1 33 159.1 2832088 95.5% 2704644 271.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 469.4 / ‑6.7 142I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (66/74);  total (67/75)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 142 total aligned reads displayed.

TGCAGCACGGGCGTAAACTCCAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTG  >  NC_000913/2132507‑2133064
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                      
tGCAGCACGGGCGTAAACTCCAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCCGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATATGCAGGCTCTGCGt                                                                                                                                                                                                                                                                                 >  2:899935/1‑288 (MQ=255)
     caCGGGCGTAAACTCCAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCTGACGTTTGCCGTTACGCACCGACAGCAGCTTCAGCAAAGTGCGCCAGGTCATACGGTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGTGGTCGAATTTGTCTTTGTGCTTGCGGTACGAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                                                                                                                                                                             >  1:1330682/1‑287 (MQ=255)
     caCGGGCGTAAACTCCAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCTTGCGTCGcc                                                                                                                                                                                                                                                                             >  1:715287/1‑287 (MQ=255)
     caCGGGCGTAAACTCCAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACAGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                                                                                                                                                                             >  1:1413065/1‑287 (MQ=255)
     caCGGGCGTAAACTCCAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                                                                                                                                                                             >  1:1144823/1‑287 (MQ=255)
       cGGGCGTAAACTCCAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtc‑tt                                                                                                                                                                                                                                                                        <  2:1015234/289‑1 (MQ=255)
               aaCTCCAGCTGTTGGCGCGCAGATCTTCCAGCCTCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCCAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATCTTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGACGTACCCGGAGAATTTTTTCGGTGGCGAGTTGATCTGCATTTCACCGTGCTCGATATTCAAGCTCTGCGTCGCCTC‑TTCTACttt                                                                                                                                                                                                                                                                 >  2:1279372/1‑288 (MQ=255)
                    cAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCTGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCCGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGAGCTGCATTTCACCGTGATGGATATGCCGGCTCTGCGTC‑CCTCGTTCTACTTTCCAggg                                                                                                                                                                                                                                                           >  2:1181691/1‑289 (MQ=255)
                    cAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGTATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACgg                                                                                                                                                                                                                                                           >  1:186302/1‑289 (MQ=255)
                    cAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACgg                                                                                                                                                                                                                                                           >  2:330537/1‑289 (MQ=255)
                        tGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGtt                                                                                                                                                                                                                                                         <  1:875467/287‑1 (MQ=255)
                        tGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGtt                                                                                                                                                                                                                                                         <  1:1091146/287‑1 (MQ=255)
                           tGGCGCGCGAATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCTACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCGGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCCGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGAGTGATATGCAGGCTCGGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                                                                                    <  2:982248/289‑1 (MQ=255)
                                        ttCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTccaccacca                                                                                                                                                                                                                                       <  1:733746/289‑1 (MQ=255)
                                               aTCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCg                                                                                                                                                                                                                                   <  2:541814/286‑1 (MQ=255)
                                                 cTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                                                                                                                                                              >  1:1101425/1‑289 (MQ=255)
                                                  tttAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCg                                                                                                                                                                                                                                   <  2:312831/283‑1 (MQ=255)
                                                  tttAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCg                                                                                                                                                                                                                                   >  1:312831/1‑283 (MQ=255)
                                                    tAGAGCCCCCGAGTTCACTAAGCCATACGTTTTCAGTTACGGACCGCCAGAAGCGTGAGGAAAGTGCGCCAGGTTATACGGTTATGGGGGATCTGGTTGAGGGTAAACAGCTTATATTTTGTGCTGTCGCGGTCTAATTTGTCTTTGTGATTGCGGTAAAAGCGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGAACTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                                                                                           <  2:1367645/289‑1 (MQ=255)
                                                        gcCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                                                                                                                                                                         <  1:330537/287‑1 (MQ=255)
                                                           cccGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACt                                                                                                                                                                                                                                                                   >  2:958611/1‑242 (MQ=255)
                                                           cccGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACt                                                                                                                                                                                                                                                                   <  1:958611/242‑1 (MQ=255)
                                                                   ccAACAGCCAGTCTTGTGCCGTGACGCCCCGGCGGCAGCTGCAGTCAAATGGTCCTGGTCTTACGTTGATTGCGGATCTGGTATAGTGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTTCGGTAAAAGTGGAAGTACCCGGCGAATTTTTTCGGTGCCGAGGGGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                                                                                                                                                            <  2:567650/289‑1 (MQ=255)
                                                                      tCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTAATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTAACCGGAAAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGACGTACTCCACCACCATCCGCAGTAAGATGTCGTAATCCTGTAcc                                                                                                                                                                                                          >  1:1184020/1‑288 (MQ=255)
                                                                      tCAGACAGAAGCTTGCCGTTACGCCCAAACAGCAACTTAAGCCAAGTGCGCCAGGTCATGCGTTTATTGCGGATCTGGTAGAGGCTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGAGGTAAATGAGGAAGTACCCGGAGAATTTCTTCGGTGACGAGGTGCTCTGCATTTCACCGAGATCGATATGCAGGCTCTGCGTCGCCTC‑TTATCCTCTCCACGGTTCGCCCTACTCCACCACCATCCCCAGGATTATGTCGCATTCCTGTccc                                                                                                                                                                                                          >  2:508556/1‑288 (MQ=255)
                                                                              aggTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGAAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCCCCCGCCGCAGGAAGCTGTCGTAATCATGTGCCGCTTTccat                                                                                                                                                                                                 >  2:1011570/3‑286 (MQ=255)
                                                                              aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGCTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTTGCCGTACTCCACCCTCCTCCGCCGGCAGATGTCGTAATCATGTGCCGCTCTCTGt                                                                                                                                                                                                 >  2:1159338/1‑289 (MQ=255)
                                                                              aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt                                                                                                                                                                                                 >  1:91834/1‑289 (MQ=255)
                                                                               cGTTTGCGGTTAGGCACCGACATCAGAGTCAGCAAAGTGCGCCAGGTCATACGGTTATTGCGGGTCTGGTAGAGGGTAAACAGCTGATACTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTTGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                                                                                                                                                                   <  1:1372727/286‑1 (MQ=255)
                                                                               cGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                                                                                <  1:1279372/289‑1 (MQ=255)
                                                                                  ttGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                                                                                                                                                             >  2:133649/1‑289 (MQ=255)
                                                                                  ttGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                                                                                                                                                             >  1:985738/1‑289 (MQ=255)
                                                                                   tGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCGGGAGAATTTTTTGGGTGACGGGGTGATCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCCCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTATCCCTGTGCCGCTTCCAGTTCGGt                                                                                                                                                                                            >  1:1070002/1‑289 (MQ=255)
                                                                                        ttACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGTTGTCGTACTCCTGTGCCGCTTTCCGTTCGGTATCGa                                                                                                                                                                                       >  2:1360928/1‑289 (MQ=255)
                                                                                            ggaCCGACAGCAGCGTCAGCAAAGAGCGCCAGGGTATACGTTTATTGCGGGTTTGGTAGAGTGTAAGCGGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGGTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                                                                                                                                                                    <  2:1086994/286‑1 (MQ=255)
                                                                                              acggACGGCAGCGTCAGCAAATGGCGCCAGGTAATACGTTTATTGCGGATCTTGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                                                                                                                                                                  <  2:1144823/285‑1 (MQ=255)
                                                                                               ccGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                                                                                <  1:899935/289‑1 (MQ=255)
                                                                                                  aCAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGTTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCTCGGTTCGCCGTACTCCCCCACCATCCGCAGGAACTTGTCGTACTCCTGTTCCTCTTTCAGTTCGGTATCGCACAAACActt                                                                                                                                                                             >  2:678332/1‑288 (MQ=255)
                                                                                                    agcagcGTCAAGAAAGTGGGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGGAAAAAGCTGATATTTTTTGCTGGCGGGGTCGAATTTGTTTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTTCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                                                                                <  1:760607/268‑1 (MQ=255)
                                                                                                          gTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc                                                                                                                                                                     >  1:826861/1‑289 (MQ=255)
                                                                                                             aGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc                                                                                                                                                                  <  2:1101425/289‑1 (MQ=255)
                                                                                                              gCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCg                                                                                                                                                                                                                                                       <  1:444108/203‑1 (MQ=255)
                                                                                                              gCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                                                                                                                                                                 >  2:1282705/1‑289 (MQ=255)
                                                                                                                aaaTTGCGCCAGGTCATACGTTTATTGCGGAGCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                                                                                                                                                                 <  1:313660/287‑1 (MQ=255)
                                                                                                                   ctGCGCCAGGTCATACGTTTATTGCCGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTTGCGCGGTCGATTTTGTCTCTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTAATCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                                                            <  1:1253742/288‑1 (MQ=255)
                                                                                                                    tGCGCCGGGGCATACGTTGATTGCGGATGTGGTAGAGGGTAAACAGCTGTTATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATGTGCAGGCTCTGCGTCGCCTC‑TTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                                                            <  2:433645/288‑1 (MQ=255)
                                                                                                                         cAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTGTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaa                                                                                                                                                      <  2:826861/289‑1 (MQ=255)
                                                                                                                             tCATACGTTTGTTGCGGGTCTGGTAGAGGGTAAACATCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc                                                                                                                                                  <  2:652899/289‑1 (MQ=255)
                                                                                                                                 acctttATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg                                                                                                                                              <  1:1159338/286‑1 (MQ=255)
                                                                                                                                     ttATTGCGGATCTGGTAGAGGGTAAACAGCTGATATGTTTGGCTGGGGCGGTCGATTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGc                                                                                                                                          <  2:210350/289‑1 (MQ=255)
                                                                                                                                      tATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                                                            <  1:1360277/270‑1 (MQ=255)
                                                                                                                                                 tGGTAGAGGGTAAACAGCTGATATTGTTTGCTGTCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTTATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAATACCTGGTTGCCAATGATATTGc                                                                                                                              <  1:1011570/289‑1 (MQ=255)
                                                                                                                                                     agagTGTAAACACCTGTTATGTTTTTCTGGGGCGGGCGAATTTGTCGTTTTGGTTGCGGTAAAAGTGGAAGTACCCGGAGAATTGTTTCGTTGACGATGTGATCTGCATTTCACCTGGATTGATAGCCAGGCTGTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGttt                                                                                                                          <  1:1308817/289‑1 (MQ=255)
                                                                                                                                                        gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTa                                                                                                                       >  1:801136/1‑289 (MQ=255)
                                                                                                                                                         ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAg                                                                                                                      >  1:725080/1‑289 (MQ=255)
                                                                                                                                                         ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAg                                                                                                                      >  1:544369/1‑289 (MQ=255)
                                                                                                                                                         ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAg                                                                                                                      >  2:1322564/1‑289 (MQ=255)
                                                                                                                                                             aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACa                                                                                                                  >  2:793540/1‑289 (MQ=255)
                                                                                                                                                             aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACa                                                                                                                  >  1:1033509/1‑289 (MQ=255)
                                                                                                                                                             aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACa                                                                                                                  >  1:979498/1‑289 (MQ=255)
                                                                                                                                                             aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGATTGCGGTAAAAGTGGAAGTACCGGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAGTGATTGATATTCAGGCTCTGCGTCGCCTC‑TTCTACTTTcta                                                                                                                                                                                                                                                              >  1:691517/1‑147 (MQ=255)
                                                                                                                                                             aaCAGCTGATATTTTTTGCTGGCGCGGACGAATTTGTCGCTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTCCTTTCCACGCTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAAAACTATTTGACACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGCATTTTCGTTTGTAGAACa                                                                                                                  >  2:1388312/1‑289 (MQ=255)
                                                                                                                                                                  cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                                                                            <  1:673262/242‑1 (MQ=255)
                                                                                                                                                                   tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAggcggc                                                                                                            >  1:364574/1‑289 (MQ=255)
                                                                                                                                                                    gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACaggaggag                                                                                                           >  1:1336037/1‑287 (MQ=255)
                                                                                                                                                                     atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGt                                                                                                          >  2:413800/1‑289 (MQ=255)
                                                                                                                                                                          ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATa                                                                                                     <  2:801136/289‑1 (MQ=255)
                                                                                                                                                                            ttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACg                                                                                                   >  2:350957/1‑289 (MQ=255)
                                                                                                                                                                            ttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACg                                                                                                   <  1:133649/289‑1 (MQ=255)
                                                                                                                                                                                 gcggtgtgtgAATTTGTTTTTGTGCTTTCGGGAAAAATTGACGTACCCGGGGAATTTTTTCGGTGACTTGCTTATCTGCATTTCACCGTGATTGATATGCAGACTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGAt                                                                                              <  2:1041247/280‑1 (MQ=255)
                                                                                                                                                                                  gggcgGTCGTATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCAGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCGTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGAt                                                                                              <  1:1388312/286‑1 (MQ=255)
                                                                                                                                                                                   cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGAttt                                                                                            >  2:1168283/1‑289 (MQ=255)
                                                                                                                                                                                   cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGAtt                                                                                             <  2:717567/288‑1 (MQ=255)
                                                                                                                                                                                            aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACa                                                                                    <  2:364574/288‑1 (MQ=255)
                                                                                                                                                                                                     tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                                                                                <  1:547614/92‑1 (MQ=255)
                                                                                                                                                                                                     tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGcc                                                                                                                                         <  2:452947/226‑1 (MQ=255)
                                                                                                                                                                                                     tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGcc                                                                                                                                         >  1:452947/1‑226 (MQ=255)
                                                                                                                                                                                                           tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg                                                                                                                                              <  1:548600/215‑1 (MQ=255)
                                                                                                                                                                                                           tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg                                                                                                                                              >  2:548600/1‑215 (MQ=255)
                                                                                                                                                                                                             cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGt                                                                   <  2:544369/288‑1 (MQ=255)
                                                                                                                                                                                                             aggtggaaGTGGAAGTGCCAGGAGAATTGTTTCGTTGACGAAGTGATGTGCGTGTCCCCGGGTTTGATATGCAGGCTCTGGTTCGCCGT‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCGGGAATATGGCGTAATCCTGTGCCGCTTTCACTGCGGTATCGAACAAACCCTCTTTGACAGGCGATGCCCAGGTAAAGAGCTGGTTGCCAATGATATTGCGTTTGTAGACCGGGCGGCGTGAATAAGGCGATTTGGGATACACCGGCACGATTGCCGGt                                                                   <  2:317789/282‑1 (MQ=255)
                                                                                                                                                                                                                         aaGTACCCGGCGAATTTTTTCGGTGACGAGGTGATCTGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACt                                                       <  1:1156336/288‑1 (MQ=255)
                                                                                                                                                                                                                             aCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCg                                                    <  1:1322564/287‑1 (MQ=255)
                                                                                                                                                                                                                              cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATCCGGCGATTTGGGATACAGCGGCAAGCCTGCCGGTTGGGAATGCACTTCgg                                                   >  2:557207/1‑286 (MQ=255)
                                                                                                                                                                                                                              cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGc                                                   >  1:371191/1‑287 (MQ=255)
                                                                                                                                                                                                                                cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaac                                                                                                                                                                                 >  1:645846/1‑159 (MQ=255)
                                                                                                                                                                                                                                             cGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCg                                  <  2:725080/289‑1 (MQ=255)
                                                                                                                                                                                                                                                 gtccgtgggATCTGACTTGCACGCTGATTGTTATGCACGCTCTGCGTCCCCTC‑TTCCACTTTCCACTGTTCGCCGTGCTCCTCCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTGCAGTATCGAACAAACACTCGTTGAATCGCCATGCCCTGGTGTAGACCGGTTTGCAAATGAAATTGCGTTTGTAGAACAGGCGGCGTGAATACTGCGATTTTTGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTa                               <  2:266201/280‑1 (MQ=255)
                                                                                                                                                                                                                                                            cTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCTCCGTACTCCACCCACATCCGCAGGCAGATGTCGTCATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCCTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCCTGACTACGGAGATTTGGGATACAGCGGCCAGCTTGCCGGTTGGGAATAGACTTCGTCCTGGCAAACGTAGTCGGTACCGGACGAAAAg                   >  2:268090/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  ttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCgtgt             >  2:1102440/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       cGTGATTGGAATGCAAGCTCTCCGGCTCCTC‑TTCTACTTTCAACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCGGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTGGAAACGCCATGCCCAGGTAAAGACCTGGGTGCAAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGGTGGGAAGAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGAc        <  2:1264146/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                         tgattgatATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCa      >  1:201053/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         tgattgatATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCa      >  2:215818/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          gattgatATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGT‑AAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGt    <  2:91834/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            ttgatAGGCAGGCTCGTCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTGTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGAGTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGtt   <  1:1277352/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tgatATGCAGGCTCTGCGTCTCCTC‑TTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTGCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTg  <  2:1047498/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tgatATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTg  <  1:787590/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             tgatATGCAGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTg  <  1:1360928/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gtatCCAGGACCTGCGTGGCCTC‑GTCCAGTTTCCACGGTTCGCCCTACTCCAGCCCCTTCCACAGGAAGGAGTCGGAATCCTGTGCCGCTTTCAGGTCGGTTTCCAACAATAGCTCTTTGAGACGCCATGCCCAGGTAAAGACCAGGTTGCCAATGATAGTGCGTTTGTAGAACAGGCGTGGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTACGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCTTGTTTGACCAGTTg  <  2:496710/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     aGGCTCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAggc                                                                                                               <  1:689834/172‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                      
TGCAGCACGGGCGTAAACTCCAGCTGTTGGCGCGCAGATCTTCCAGCATCTTTAGCGCCCCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTC‑TTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTG  >  NC_000913/2132507‑2133064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A