Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F110 I1 R1	18	47.7	1781824	90.5%	1612550	142.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	97.8%	131.1 / NA	46	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (24/21); minor base G (1/0); total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132648‑2132925

gATCTGGTAGATGGTAAACAGCTGATATGTTTTGCTGGCGCGGTCGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct                                                                                                                                         <  2:219137/143‑1 (MQ=255)

gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGctct                                                                                                                                         <  1:559630/143‑1 (MQ=255)

 aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg                                                                                                                                        >  2:416178/1‑143 (MQ=255)

     ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTTATATGCAGGCTCTGCGTc                                                                                                                                    >  2:490558/1‑143 (MQ=255)

       tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTCAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTccc                                                                                                                                  >  2:789277/1‑141 (MQ=255)

                 aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGGATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt                                                                                                                        >  1:554635/1‑143 (MQ=255)

                      tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttttcg                                                                                                                   >  1:443953/1‑140 (MQ=255)

                        atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                                 >  1:451822/1‑143 (MQ=255)

                                tGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGCGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                         <  1:285408/143‑1 (MQ=255)

                                  cTGGCGCGGTCGAATTTGTCTTTGTACTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGGATTGATATGCAGCGTCTGCGTCGCCGCTTATACTCTCCACGGTCAGCCgga                                                                                                       >  2:647638/1‑141 (MQ=255)

                                  cTGGCGCGGTCGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                       <  1:493755/143‑1 (MQ=255)

                                        cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                 <  1:801744/143‑1 (MQ=255)

                                                          tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt                                                                               <  1:500691/143‑1 (MQ=255)

                                                           gCTTTCGGTATAAGTGGAAGTATCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTCCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                              <  2:505078/143‑1 (MQ=255)

                                                           gCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCCTCTCCTTTCCACGGTTAGCCGTACTCCACCACCCTCCGGAGGGAGCTg                                                                              >  2:325620/1‑143 (MQ=255)

                                                            cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTAGCCTATACTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt                                                                             >  1:284832/1‑143 (MQ=255)

                                                              cgCGGTAAAAGTGGAAGTACCCGGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                           <  1:98645/142‑1 (MQ=255)

                                                                 ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                        <  1:733812/143‑1 (MQ=255)

                                                                  gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt                                                                       <  1:862005/143‑1 (MQ=255)

                                                                   tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATACAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCCCAGGAAGATGTCGTAATc                                                                      >  2:444176/1‑143 (MQ=255)

                                                                       aGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGCTGTCGTAATCCtgt                                                                  >  1:594145/1‑143 (MQ=255)

                                                                        gTGGAAGCACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                 <  1:159421/143‑1 (MQ=255)

                                                                                aCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGACGTCGTAATCCTGCGCCGCttcc                                                         >  2:603192/1‑141 (MQ=255)

                                                                                  ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCtctccg                                                       >  1:12543/1‑141 (MQ=255)

                                                                                  ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCtctacg                                                       >  2:136404/1‑138 (MQ=255)

                                                                                    ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTCCGCAGGAGGAAGTCGTAGCCGCGTGCCGCTTTCAGtt                                                     >  2:764389/1‑143 (MQ=255)

                                                                                     gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCACGGTTCGCCGTACTCAACCACCCTCCGCTGGGAGAGGTCGTAATCCTGTCCCGCTGTCAGTTc                                                    >  1:838164/1‑143 (MQ=255)

                                                                                        aaTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCCTTCAGTTCGGt                                                 >  2:227511/1‑143 (MQ=255)

                                                                                        aaTTTTTTCGGTGACGAGGTGATCTGCAATTCACCGTGTATGTTATTTAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTTGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGGTTTCAGTTCGGt                                                 >  1:845351/1‑143 (MQ=255)

                                                                                        aaTTTTTTCGGAGGCGAGTTGATCTGCATTTCTCCGTTATTGATATGCAGGCTCTGCGACGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt                                                 <  2:785262/143‑1 (MQ=255)

                                                                                          ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCTCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCCGTGCCGCTTTCCGTTCGGTAt                                               >  2:442352/1‑143 (MQ=255)

                                                                                           tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                              <  1:416178/143‑1 (MQ=255)

                                                                                              ttCGGTGACGAGGTAATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                           <  2:456606/143‑1 (MQ=255)

                                                                                                 ggTGACGAGGTGATCTGCATTTCATCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGCCGCTGTTGTCATCCTTTGCTGCTTTCAGTTAGGTAttaaaaaa                                        >  1:13991/1‑136 (MQ=255)

                                                                                                       gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCATGTGCCGCTTTCAGTTAGGTAGCGAACAAACActa                                  >  2:128237/1‑142 (MQ=255)

                                                                                                         ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                                <  1:636412/143‑1 (MQ=255)

                                                                                                         ggTGATCTGCACTTCACCGTGATTGACATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                                <  2:149432/143‑1 (MQ=255)

                                                                                                         ggTGAGCTCCTTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                                <  2:339310/143‑1 (MQ=255)

                                                                                                              tCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                           <  1:226877/143‑1 (MQ=255)

                                                                                                                 gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCATACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                        <  1:695662/143‑1 (MQ=255)

                                                                                                                     gtacccGTGAGTGACATGCAGGCTCTCCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCATGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATg                    <  2:605470/139‑1 (MQ=255)

                                                                                                                      tCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTCTCAGCTCGGTATCGCACAAACACACTTGCAcacgccacgc                   >  2:447028/1‑143 (MQ=255)

                                                                                                                        aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCAGACAAACGATAACGGAGTTGTGTGCCGCTTTCAGTTCGGTATCGCACAACCACTCTTTGCACCGCCATGccc                 >  1:409783/1‑143 (MQ=255)

                                                                                                                         ccgcgcttgctATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACTGTTCGCCGTCCTCCACCCCCATCCGCACGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCTGTATCGACCAAACACTCTTTGAAACGCCATGCCCa                <  2:743782/137‑1 (MQ=255)

                                                                                                                          cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGGTGTCGGAATCCTGTGCCGCTTGCAGTTCGGTATCGAACAAACGCTCTTTGAAACGCCATGCCCAg               >  1:211782/1‑143 (MQ=255)

                                                                                                                                    atGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt     <  1:497953/143‑1 (MQ=255)

                                                                                                                                      gCAGGCTCTGCGTCGCCGCGTCTACTTTCCACGGTCCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACTCTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg   <  1:9705/143‑1 (MQ=255)

                                                                                                                                       cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt  >  2:675710/1‑143 (MQ=255)

GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132648‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A