Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,973	T→C	G146G (GGA→GGG)	ccmH ←	heme lyase, CcmH subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,973	0	T	C	100.0%	177.6 / NA	49	G146G (GGA→GGG)	ccmH	heme lyase, CcmH subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/25); total (24/25)

GCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCA  >  NC_000913/2291839‑2292091

gcCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                    <  1:813227/139‑1 (MQ=255)

   cTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCAc                                                                                                                 <  1:705453/139‑1 (MQ=255)

         aGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                    <  1:419207/130‑1 (MQ=255)

         aGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                    >  2:419207/1‑130 (MQ=255)

           cAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCt                                                                                                         >  2:1067448/1‑139 (MQ=255)

                ccGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCgg                                                                                                    >  2:1193382/1‑139 (MQ=255)

                    aGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACg                                                                                                <  1:529305/139‑1 (MQ=255)

                          tgCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                              >  1:780354/1‑135 (MQ=255)

                          tgCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGt                                                                                          <  2:1036178/139‑1 (MQ=255)

                          tgCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGt                                                                                          <  2:557926/139‑1 (MQ=255)

                          tgCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTACTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                              <  2:780354/135‑1 (MQ=255)

                                                        ctgATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTc                                                                            >  1:1005277/1‑123 (MQ=255)

                                                        ctgATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTc                                                                            <  2:1005277/123‑1 (MQ=255)

                                                        ctgATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTAcc                                                            >  1:141764/1‑139 (MQ=255)

                                                                  ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                 >  2:475618/1‑108 (MQ=255)

                                                                  ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                 <  1:475618/108‑1 (MQ=255)

                                                                    aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                <  1:1067448/139‑1 (MQ=255)

                                                                     gTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGc                                               >  1:438205/1‑139 (MQ=255)

                                                                       cTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCgg                                             <  2:1293627/139‑1 (MQ=255)

                                                                                gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTc                                                                            <  1:496959/99‑1 (MQ=255)

                                                                                gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                >  1:466487/1‑127 (MQ=255)

                                                                                gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                <  2:466487/127‑1 (MQ=255)

                                                                                gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                            >  1:1161704/1‑131 (MQ=255)

                                                                                gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                            <  2:1161704/131‑1 (MQ=255)

                                                                                gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACACGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTc                                                                            >  2:496959/1‑99 (MQ=255)

                                                                                    aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                    >  2:273916/1‑55 (MQ=255)

                                                                                    aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                    <  1:273916/55‑1 (MQ=255)

                                                                                    aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                            <  1:763138/127‑1 (MQ=255)

                                                                                    aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                            >  2:763138/1‑127 (MQ=255)

                                                                                    aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGc                                           >  2:915721/1‑128 (MQ=255)

                                                                                    aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGc                                           <  1:915721/128‑1 (MQ=255)

                                                                                    aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAccc                                >  2:1288504/1‑139 (MQ=255)

                                                                                     cGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTAcc                                                                                                          <  2:1473825/64‑1 (MQ=255)

                                                                                     cGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTAcc                                                                                                          >  1:1473825/1‑64 (MQ=255)

                                                                                                     cGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCtt                                                                                                             >  2:285869/1‑45 (MQ=255)

                                                                                                     cGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCtt                                                                                                             <  1:285869/45‑1 (MQ=255)

                                                                                                         accacAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCAcgc                                                                 <  2:1376689/85‑1 (MQ=255)

                                                                                                         accacAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCAcgc                                                                 >  1:1376689/1‑85 (MQ=255)

                                                                                                           cacaATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCact         >  1:1167173/1‑139 (MQ=255)

                                                                                                                tACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTgg    >  1:1159093/1‑139 (MQ=255)

                                                                                                                  cccGGCAGATAAACAACATACCCGGCACGCGTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGgca  <  2:1159093/139‑1 (MQ=255)

                                                                                                                     ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcaccac                                                                   >  1:451836/1‑71 (MQ=255)

                                                                                                                     ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcaccac                                                                   <  2:451836/71‑1 (MQ=255)

                                                                                                                        aGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCa                                                                                       <  2:591545/48‑1 (MQ=255)

                                                                                                                        aGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCa                                                                                       >  1:591545/1‑48 (MQ=255)

                                                                                                                        aGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAGACGCTTCCGGCACCACGCGTACCCGAcgcc                                                     >  1:299284/1‑82 (MQ=255)

                                                                                                                        aGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAGACGCTTCCGGCACCACGCGTACCCGAcgcc                                                     <  2:299284/82‑1 (MQ=255)

                                                                                                                            aaaCAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                 <  1:310762/50‑1 (MQ=255)

                                                                                                                            aaaCAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                 >  2:310762/1‑50 (MQ=255)

                                                                                                                                   atacCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                            >  2:717707/1‑80 (MQ=255)

                                                                                                                                   atacCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                            <  1:717707/80‑1 (MQ=255)

GCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCA  >  NC_000913/2291839‑2292091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A