Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	780	68.3	3343050	93.5%	3125751	102.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,973	T→C	G146G (GGA→GGG)	ccmH ←	heme lyase, CcmH subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,973	0	T	C	100.0%	206.0 / NA	59	G146G (GGA→GGG)	ccmH	heme lyase, CcmH subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/28); total (31/28)

GCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGC  >  NC_000913/2291849‑2292102

gCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTAcc                                                                                                                     <  1:612318/139‑1 (MQ=255)

      ccGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCtt                                                                                                                   >  2:370065/1‑135 (MQ=255)

      ccGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCtt                                                                                                                   <  1:370065/135‑1 (MQ=255)

                    gTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAg                                                                                                 >  1:951946/1‑139 (MQ=255)

                     tGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTcggg                                                                                                >  2:568001/1‑136 (MQ=255)

                     tGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAgg                                                                                                >  1:1589748/1‑139 (MQ=255)

                      ggCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGa                                                                                               <  2:951946/139‑1 (MQ=255)

                         ctgctgCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCGGGAAAc                                                                                            <  1:568001/139‑1 (MQ=255)

                                         aCCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCa                                                                                                                             <  1:117484/90‑1 (MQ=255)

                                         aCCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCa                                                                                                                             >  2:117484/1‑90 (MQ=255)

                                         aCCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                         <  2:1278693/110‑1 (MQ=255)

                                         aCCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                         >  1:1278693/1‑110 (MQ=255)

                                              ctgATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATAccc                                                                                                                                 <  1:1067146/81‑1 (MQ=255)

                                                     ttGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                            >  2:1293054/1‑111 (MQ=255)

                                                     ttGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                            <  1:1293054/111‑1 (MQ=255)

                                                        ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCcgcg                                                             >  2:929954/1‑139 (MQ=255)

                                                        ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCcgcg                                                             >  1:1000975/1‑139 (MQ=255)

                                                        ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCcgcg                                                             >  2:238785/1‑139 (MQ=255)

                                                         cAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGa                                                            <  1:359390/139‑1 (MQ=255)

                                                          aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                           <  1:238785/139‑1 (MQ=255)

                                                          aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                           <  2:1000975/139‑1 (MQ=255)

                                                           gTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACACTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGc                                                          >  1:1510098/1‑139 (MQ=255)

                                                                gtagtaGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCg                                                     >  1:1642021/1‑139 (MQ=255)

                                                                 tagtagCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                            >  1:1595604/1‑99 (MQ=255)

                                                                 tagtagCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                            <  2:1595604/99‑1 (MQ=255)

                                                                      gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                         <  1:491926/81‑1 (MQ=255)

                                                                      gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                         >  2:491926/1‑81 (MQ=255)

                                                                      gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATg                                               >  2:514668/1‑139 (MQ=255)

                                                                          aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACg                                                                                                           >  2:1629002/1‑75 (MQ=255)

                                                                          aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACg                                                                                                           <  1:1629002/75‑1 (MQ=255)

                                                                          aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                                       >  1:1215792/1‑127 (MQ=255)

                                                                          aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                                       <  2:1215792/127‑1 (MQ=255)

                                                                          aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGta                                                   <  1:552702/131‑1 (MQ=255)

                                                                          aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGta                                                   >  2:552702/1‑131 (MQ=255)

                                                                          aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAccc                                           >  1:893440/1‑139 (MQ=255)

                                                                          aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAccc                                           >  2:1274670/1‑139 (MQ=255)

                                                                                  cacaATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgcca                                   <  2:1642021/139‑1 (MQ=255)

                                                                                  cacaATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgcca                                   <  1:1653733/139‑1 (MQ=255)

                                                                                       ttAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgccaatgcc                              <  2:1589748/139‑1 (MQ=255)

                                                                                          aCGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCg                                                     <  1:1564057/113‑1 (MQ=255)

                                                                                          aCGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCg                                                     >  2:1564057/1‑113 (MQ=255)

                                                                                                        cccGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGgca             >  2:1553959/1‑139 (MQ=255)

                                                                                                           ggCAGATAAACAACATACGCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgccaatgccaa                            >  2:1099652/1‑121 (MQ=255)

                                                                                                           ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCtt                                                                                                        <  1:827995/45‑1 (MQ=255)

                                                                                                           ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCtt                                                                                                        >  2:827995/1‑45 (MQ=255)

                                                                                                           ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCTCCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgccaatgccaa                            <  1:1099652/121‑1 (MQ=255)

                                                                                                           ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                           >  1:625932/1‑90 (MQ=255)

                                                                                                           ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                           <  2:625932/90‑1 (MQ=255)

                                                                                                           ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGgtagca          >  1:690631/1‑139 (MQ=255)

                                                                                                             cAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGaa                                                                                              >  2:1006096/1‑53 (MQ=255)

                                                                                                             cAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGaa                                                                                              <  1:1006096/53‑1 (MQ=255)

                                                                                                             cAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCAcc        <  2:893440/139‑1 (MQ=255)

                                                                                                             cAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCAcc        >  1:927795/1‑139 (MQ=255)

                                                                                                             cAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACACTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCAcc        <  2:1510098/139‑1 (MQ=255)

                                                                                                              aGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATg                                               >  2:207894/1‑99 (MQ=255)

                                                                                                              aGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATg                                               <  1:207894/99‑1 (MQ=255)

                                                                                                                aTAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                         >  1:1311987/1‑39 (MQ=255)

                                                                                                                aTAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                         <  2:1311987/39‑1 (MQ=255)

                                                                                                                   aacaacATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCAcagc  <  1:1553959/139‑1 (MQ=255)

                                                                                                                   aacaacATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCAcagc  >  2:1507695/1‑139 (MQ=255)

GCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGC  >  NC_000913/2291849‑2292102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A