Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,973	T→C	G146G (GGA→GGG)	ccmH ←	heme lyase, CcmH subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,973	0	T	C	100.0%	199.4 / NA	55	G146G (GGA→GGG)	ccmH	heme lyase, CcmH subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/26); total (29/26)

TCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCAC  >  NC_000913/2291845‑2292099

tCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCt                                                                                                                      >  2:833853/1‑139 (MQ=255)

        cGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                            <  1:1178659/125‑1 (MQ=255)

        cGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                            >  2:1178659/1‑125 (MQ=255)

           cGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGc                                                                                                           <  2:210769/139‑1 (MQ=255)

              aGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACg                                                                                                        <  1:833853/139‑1 (MQ=255)

                  tgtgCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCtt                                                                                                                >  2:931461/1‑127 (MQ=255)

                  tgtgCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCtt                                                                                                                <  1:931461/127‑1 (MQ=255)

                          ggCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTTACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACACACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGa                                                                                            <  1:1587249/139‑1 (MQ=255)

                            ccTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGaaa                                                                                          >  2:534679/1‑139 (MQ=255)

                             ctgctgCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                         <  1:534679/139‑1 (MQ=255)

                                      gATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGc                                                                                                                           <  1:638079/96‑1 (MQ=255)

                                      gATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGc                                                                                                                           >  2:638079/1‑96 (MQ=255)

                                                 gctgATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTAc                                                                     >  2:906704/1‑139 (MQ=255)

                                                  ctgATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTAcc                                                                    >  2:1392769/1‑139 (MQ=255)

                                                        aTTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGAcgc                                                              >  1:1401337/1‑139 (MQ=255)

                                                            ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTAcc                                                                                                                  >  2:516568/1‑83 (MQ=255)

                                                            ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTAcc                                                                                                                  <  1:516568/83‑1 (MQ=255)

                                                              aGTCTGGTAGTAGCTGATGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTACAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        <  1:1065856/139‑1 (MQ=255)

                                                                tCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGaa                                                                                           >  1:1140216/1‑102 (MQ=255)

                                                                tCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGaa                                                                                           <  2:1140216/102‑1 (MQ=255)

                                                                          gCTGACGCCAGCCACAATTACCGCCACCACAATACCCGCCAGATAAATAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCTCCACGCGTACCCGCCGCCGCGAGCGGTCGTATATg                                            >  1:1276232/1‑139 (MQ=255)

                                                                              aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAccc                                        >  2:274348/1‑139 (MQ=255)

                                                                              aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAccc                                        >  2:1642319/1‑139 (MQ=255)

                                                                               cGCCAGCCACAGTTAACGCCACCACAATACCCGGCAGATAAACAACATAcccag                                                                                                                            >  2:1004476/1‑52 (MQ=255)

                                                                               cGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                            <  1:1004476/54‑1 (MQ=255)

                                                                               cGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                                    <  1:728090/126‑1 (MQ=255)

                                                                               cGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCggg                                                    >  2:728090/1‑126 (MQ=255)

                                                                               cGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCa                                       <  1:1642319/139‑1 (MQ=255)

                                                                                gccagccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCaa                                      <  1:274348/139‑1 (MQ=255)

                                                                                    gccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCAGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgc                                  >  2:997106/1‑139 (MQ=255)

                                                                                     ccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgcc                                 >  2:1332073/1‑139 (MQ=255)

                                                                                            tAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgccaatgcca                          <  1:1392769/139‑1 (MQ=255)

                                                                                                   accacAATACCCGGCAGATAAACAACATACCCGGCa                                                                                                                          >  1:681428/1‑36 (MQ=255)

                                                                                                   accacAATACCCGGCAGATAAACAACATACCCGGCa                                                                                                                          <  2:681428/36‑1 (MQ=255)

                                                                                                   accacAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcac                                                                              <  1:1541100/80‑1 (MQ=255)

                                                                                                   accacAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcac                                                                              >  2:1541100/1‑80 (MQ=255)

                                                                                                   accacAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        <  2:627383/102‑1 (MQ=255)

                                                                                                   accacAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        >  1:627383/1‑102 (MQ=255)

                                                                                                     cacaATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCact                 >  1:99265/1‑139 (MQ=255)

                                                                                                       caATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCactac               <  1:1332073/139‑1 (MQ=255)

                                                                                                               ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        <  1:1362607/90‑1 (MQ=255)

                                                                                                               ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        >  2:1362607/1‑90 (MQ=255)

                                                                                                               ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCgg                                                     >  2:1731496/1‑93 (MQ=255)

                                                                                                               ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCgg                                                     <  1:1731496/93‑1 (MQ=255)

                                                                                                                 cAGATAAACAACATTCCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcaccac                                                                           <  2:353857/69‑1 (MQ=255)

                                                                                                                 cAGATAAACAACATTCCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcaccac                                                                           >  1:353857/1‑69 (MQ=255)

                                                                                                                   gATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCca   >  2:1527051/1‑139 (MQ=255)

                                                                                                                    aTAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCcac  >  1:1104005/1‑139 (MQ=255)

                                                                                                                      aaaCAACATACCCGGTACGCTTACCTTCCGGCACGCTTTGt                                                                                                  >  2:1474759/1‑41 (MQ=255)

                                                                                                                      aaaCAACATACCCGGTACGCTTACCTTCCGGCACGCTTTGt                                                                                                  <  1:1474759/41‑1 (MQ=255)

                                                                                                                      aaaCAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        >  1:830114/1‑83 (MQ=255)

                                                                                                                      aaaCAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        <  2:830114/83‑1 (MQ=255)

                                                                                                                       aacaacATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        >  2:5456/1‑82 (MQ=255)

                                                                                                                       aacaacATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                        <  1:5456/82‑1 (MQ=255)

                                                                                                                           acaTACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTAccc                                                                   >  2:398698/1‑67 (MQ=255)

                                                                                                                           acaTACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTAccc                                                                   <  1:398698/67‑1 (MQ=255)

TCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCAC  >  NC_000913/2291845‑2292099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A