Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,562	(G)_6→7	coding (126/1815 nt)	yjgL →	SopA‑central‑domain‑like hexapeptide repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,556	1	.	G	98.5%	230.5 / ‑3.5	66	coding (120/1815 nt)	yjgL	SopA‑central‑domain‑like hexapeptide repeat protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (35/30); total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

CTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCAT  >  NC_000913/4475422‑4475688

cTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑gggg                                                                                                                                  >  2:1021138/1‑139 (MQ=255)

 tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                                 <  1:394120/139‑1 (MQ=255)

 tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                                 >  1:267459/1‑139 (MQ=255)

   aaGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               <  1:402572/139‑2 (MQ=255)

   aaGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               <  1:297061/139‑2 (MQ=255)

         ttCGTCATGAGCAAAATGTCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACTAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               >  2:918003/1‑132 (MQ=255)

         ttCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               <  1:918003/133‑2 (MQ=255)

           cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATaa                                                                                                                        >  2:1093420/1‑139 (MQ=255)

           cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATaa                                                                                                                        >  1:806836/1‑139 (MQ=255)

              cATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAg                                                                                                                     <  2:1173008/139‑1 (MQ=255)

              cATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAg                                                                                                                     <  2:674859/139‑1 (MQ=255)

              cATGAGCAAAACATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAg                                                                                                                     <  2:962772/139‑1 (MQ=255)

               aTGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGa                                                                                                                    >  2:993032/1‑139 (MQ=255)

                 gAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAg                                                                                                                  >  2:81089/1‑139 (MQ=255)

                 gAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAg                                                                                                                  <  2:806836/139‑1 (MQ=255)

                          atCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGATGGAATAttct                                                                                                         <  1:1021138/139‑1 (MQ=255)

                             aGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACATGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                                 >  1:563313/1‑111 (MQ=255)

                             aGATTTAAAGTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                                 <  2:563313/111‑1 (MQ=255)

                                 ttAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCt                                                                                                  >  1:501737/1‑139 (MQ=255)

                                         ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGTCG‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtt                                                                                          >  1:544849/1‑139 (MQ=255)

                                         ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtt                                                                                          >  1:552916/1‑139 (MQ=255)

                                           ctcAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               >  2:1433659/1‑98 (MQ=255)

                                           ctcAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               <  1:1433659/99‑2 (MQ=255)

                                              aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGAt                                                                                                                          >  2:328447/1‑102 (MQ=255)

                                              aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGAt                                                                                                                          <  1:328447/102‑1 (MQ=255)

                                              aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTag                                                                                     >  1:1475540/1‑139 (MQ=255)

                                              aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTag                                                                                     >  2:405062/1‑139 (MQ=255)

                                                     tacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttttt                                                                              >  2:185176/1‑139 (MQ=255)

                                                        attaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGc                                                                           >  2:1054196/1‑139 (MQ=255)

                                                          taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAat                                                                                                              <  1:557219/102‑1 (MQ=255)

                                                          taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAat                                                                                                              >  2:557219/1‑102 (MQ=255)

                                                          taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaa                                                                         >  2:466539/1‑139 (MQ=255)

                                                          taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaa                                                                         >  2:798242/1‑139 (MQ=255)

                                                          taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaa                                                                         >  1:232025/1‑139 (MQ=255)

                                                           aGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        <  2:239923/139‑1 (MQ=255)

                                                            gCCAACAATTTTAAACAAAAGAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTGAGAGTTTTTGGCAAAg                                                                       >  2:999886/1‑139 (MQ=255)

                                                             ctgACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGGTAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGa                                                                      >  2:319004/3‑139 (MQ=255)

                                                             ccGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAat                                                                                                              <  1:182139/99‑1 (MQ=255)

                                                             ccGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAat                                                                                                              >  2:182139/1‑99 (MQ=255)

                                                               gACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaa                                                                         >  2:488477/1‑134 (MQ=255)

                                                               gACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaa                                                                         <  1:488477/134‑1 (MQ=255)

                                                               gACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGata                                                                    >  1:436732/1‑139 (MQ=255)

                                                                aCAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                   <  1:185176/139‑1 (MQ=255)

                                                                aCAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                   <  1:466539/139‑1 (MQ=255)

                                                                 cAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        <  2:478916/133‑1 (MQ=255)

                                                                 cAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        >  1:478916/1‑133 (MQ=255)

                                                                 cAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATAtt                                                                  >  2:341872/1‑139 (MQ=255)

                                                                                    gAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGAt                                               <  1:1093420/139‑1 (MQ=255)

                                                                                      aGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGAttt                                             >  1:428222/1‑139 (MQ=255)

                                                                                             aaTACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                      <  2:428222/139‑1 (MQ=255)

                                                                                               tACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACg                                    <  2:267459/139‑1 (MQ=255)

                                                                                                  cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCt                                                            >  2:1370709/1‑112 (MQ=255)

                                                                                                  cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCt                                                            <  1:1370709/112‑1 (MQ=255)

                                                                                                  cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               <  2:1158801/44‑2 (MQ=255)

                                                                                                  cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               >  1:1158801/1‑43 (MQ=255)

                                                                                                    ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        <  2:1433666/98‑1 (MQ=255)

                                                                                                    ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        >  1:883554/1‑98 (MQ=255)

                                                                                                    ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        <  2:883554/98‑1 (MQ=255)

                                                                                                    ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        >  1:1433666/1‑98 (MQ=255)

                                                                                                    ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCa                               >  1:820910/1‑139 (MQ=255)

                                                                                                    ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               >  2:994112/1‑41 (MQ=255)

                                                                                                    ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                               <  1:994112/42‑2 (MQ=255)

                                                                                                         gtTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                          <  1:1224011/91‑1 (MQ=255)

                                                                                                         gtTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                          >  2:1224011/1‑91 (MQ=255)

                                                                                                         gtTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGaa                          <  1:993032/139‑1 (MQ=255)

                                                                                                            ggAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGa                                                                                                                <  1:59011/50‑1 (MQ=255)

                                                                                                            ggAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGa                                                                                                                >  2:59011/1‑50 (MQ=255)

                                                                                                            ggAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAg                                                                                              <  1:1136784/68‑1 (MQ=255)

                                                                                                            ggAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAg                                                                                              >  2:1136784/1‑68 (MQ=255)

                                                                                                              aatgaatgATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCggg                     <  1:745640/139‑1 (MQ=255)

                                                                                                                  aatgaTTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGa                 >  2:12480/1‑139 (MQ=255)

                                                                                                                        tttGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        >  2:578380/1‑78 (MQ=255)

                                                                                                                        tttGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                        <  1:578380/78‑1 (MQ=255)

                                                                                                                        tttGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatg           >  1:1376099/1‑139 (MQ=255)

                                                                                                                        tttGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatg           <  1:999886/139‑1 (MQ=255)

                                                                                                                         ttGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatga          <  2:1475540/139‑1 (MQ=255)

                                                                                                                            cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                   >  2:773597/1‑79 (MQ=255)

                                                                                                                            cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGatat                                                                   <  1:773597/79‑1 (MQ=255)

                                                                                                                            cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg       <  1:798242/139‑1 (MQ=255)

                                                                                                                            cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATAATTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg       <  1:1440575/139‑1 (MQ=255)

                                                                                                                                 aTTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCat  >  2:70805/1‑139 (MQ=255)

CTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCAT  >  NC_000913/4475422‑4475688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A