Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,562	(G)_6→7	coding (126/1815 nt)	yjgL →	SopA‑central‑domain‑like hexapeptide repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,556	1	.	G	98.5%	231.5 / ‑3.6	66	coding (120/1815 nt)	yjgL	SopA‑central‑domain‑like hexapeptide repeat protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (31/34); total (32/34)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTC  >  NC_000913/4475423‑4475686

tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                               >  2:995084/1‑139 (MQ=255)

tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                               >  1:1356565/1‑139 (MQ=255)

tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                               >  2:517314/1‑139 (MQ=255)

tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                               <  2:1356565/139‑1 (MQ=255)

tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggg                                                                                                                               >  2:301026/1‑139 (MQ=255)

tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑gg                                                                                                                                  <  2:404734/136‑1 (MQ=255)

tCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑gg                                                                                                                                  >  1:404734/1‑136 (MQ=255)

  aaGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                             <  2:1217778/139‑2 (MQ=255)

        ttCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGAt                                                                                                                        <  1:301026/139‑1 (MQ=255)

          cGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATaa                                                                                                                      >  1:1424052/1‑139 (MQ=255)

                gAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGACTGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAg                                                                                                                >  2:401881/1‑139 (MQ=255)

                gAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                             >  2:1605505/1‑124 (MQ=255)

                gAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑ggggggg                                                                                                                             <  1:1605505/125‑2 (MQ=255)

                       atatCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGa                                                                                                                         <  1:1612411/123‑1 (MQ=255)

                       atatCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGa                                                                                                                         >  2:1612411/1‑123 (MQ=255)

                           cAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATAttcctt                                                                                                     >  2:593449/1‑139 (MQ=255)

                                    attattCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAg                                                                                            <  2:466214/139‑1 (MQ=255)

                                        ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtt                                                                                        >  2:887913/1‑139 (MQ=255)

                                        ttCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtt                                                                                        >  1:829254/1‑139 (MQ=255)

                                                  attacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGttt                                                                              >  1:996989/1‑139 (MQ=255)

                                                    tacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑gggggggcagg                                                                                                                         <  2:804316/93‑6 (MQ=255)

                                                    tacattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑gggggggcagg                                                                                                                         >  1:804316/1‑88 (MQ=255)

                                                     acattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTg                                                                           >  2:1069881/1‑139 (MQ=255)

                                                        ttaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                        <  2:829254/139‑1 (MQ=255)

                                                        ttaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                        <  2:929547/139‑1 (MQ=255)

                                                         taGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaa                                                                       >  1:202951/1‑139 (MQ=255)

                                                          aGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                      <  2:996989/139‑1 (MQ=255)

                                                          aGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                      <  2:699882/139‑1 (MQ=255)

                                                          aGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                      <  2:202951/139‑1 (MQ=255)

                                                          aGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCaaa                                                                      >  1:699882/1‑139 (MQ=255)

                                                          aGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑gg                                                                                                                                  >  2:911494/1‑78 (MQ=255)

                                                          aGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑gg                                                                                                                                  <  1:911494/78‑1 (MQ=255)

                                                           gCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAg                                                                     >  2:276044/1‑139 (MQ=255)

                                                            ccGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGa                                                                    >  1:677017/1‑139 (MQ=255)

                                                                cAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATAtt                                                                <  2:1600952/139‑1 (MQ=255)

                                                                   ttttAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTAc                                                             <  1:1069881/139‑1 (MQ=255)

                                                                        aaCAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTgg                                                        <  1:887913/139‑1 (MQ=255)

                                                                             aaaaGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTAcc                                                            <  1:263903/130‑1 (MQ=255)

                                                                             aaaaGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTAcc                                                            >  2:263903/1‑130 (MQ=255)

                                                                                            aaTACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGaa                                                                                                             <  2:52247/66‑1 (MQ=255)

                                                                                            aaTACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGaa                                                                                                             >  1:52247/1‑66 (MQ=255)

                                                                                                 cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGAt                                                                                                                        <  2:402444/50‑1 (MQ=255)

                                                                                                 cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGAt                                                                                                                        >  1:402444/1‑50 (MQ=255)

                                                                                                 cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATAttct                                                                                                       >  2:622656/1‑67 (MQ=255)

                                                                                                 cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATAttct                                                                                                       <  1:622656/67‑1 (MQ=255)

                                                                                                 cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                        >  2:855493/1‑98 (MQ=255)

                                                                                                 cTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCa                                                                        <  1:855493/98‑1 (MQ=255)

                                                                                                   ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGAt                                                                                                                        >  1:688119/1‑48 (MQ=255)

                                                                                                   ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGAt                                                                                                                        <  2:688119/48‑1 (MQ=39)

                                                                                                   ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAat                                                                                                            >  1:812733/1‑60 (MQ=255)

                                                                                                   ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAat                                                                                                            <  2:812733/60‑1 (MQ=255)

                                                                                                   ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTAtg                                                  >  1:126571/1‑118 (MQ=255)

                                                                                                   ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTAtg                                                  <  2:126571/118‑1 (MQ=255)

                                                                                                   ggCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCa                             >  2:719680/1‑139 (MQ=255)

                                                                                                     cGTGTTGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAAc                           >  2:47411/1‑139 (MQ=255)

                                                                                                         ttGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCCTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAg                       <  1:593449/139‑1 (MQ=255)

                                                                                                          tGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATAttcttt                                                                                                     <  1:245129/60‑1 (MQ=255)

                                                                                                          tGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATAttcttt                                                                                                     >  2:245129/1‑60 (MQ=255)

                                                                                                          tGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTAcc                                                            <  1:296116/101‑1 (MQ=255)

                                                                                                          tGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTAcc                                                            >  2:296116/1‑101 (MQ=255)

                                                                                                          tGGAATGAATGATTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGc                      >  2:517442/1‑139 (MQ=255)

                                                                                                              atgaatgaTTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGt                  <  1:276044/139‑1 (MQ=255)

                                                                                                                   tgaTTTTGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGaaa             >  1:1442616/1‑139 (MQ=255)

                                                                                                                       tttGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatg         <  2:1424052/139‑1 (MQ=255)

                                                                                                                        ttGCCCGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATatga        <  2:1442616/139‑1 (MQ=255)

                                                                                                                           cccGTATTGTCG‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                    >  1:300721/1‑108 (MQ=255)

                                                                                                                           cccGTATTG‑TCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                    <  2:300721/108‑1 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTa                                                                                    >  2:969109/1‑60 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTa                                                                                    <  1:969109/60‑1 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCa                                      >  1:804448/1‑106 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCa                                      <  2:804448/106‑1 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGc                      >  1:334816/1‑122 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGc                      <  2:334816/122‑1 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg     <  2:677017/139‑1 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg     >  1:23062/1‑139 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg     <  1:47411/139‑1 (MQ=255)

                                                                                                                           cccGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg     <  1:719680/139‑1 (MQ=255)

                                                                                                                             cGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                    >  1:1522830/1‑106 (MQ=255)

                                                                                                                             cGTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCaaa                                    <  2:1522830/106‑1 (MQ=255)

                                                                                                                              gTATTG‑CCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTc  <  1:995084/139‑1 (MQ=255)

TCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTG‑CC‑GGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTC  >  NC_000913/4475423‑4475686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A