Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A5 F1 I1 R1 | 770 | 51.4 | 2439994 | 93.8% | 2288714 | 110.3 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,078,438 | G→A | *330* (TAG→TAA) | yiiD → | GNAT family putative N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,078,438 | 0 | G | A | 100.0% | 138.4 / NA | 43 | *330* (TAG→TAA) | yiiD | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/22); total (21/22) | |||||||||||
AAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCA > NC_000913/4078306‑4078570 | aaaaGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATg < 1:375501/139‑1 (MQ=255) gATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgct < 1:106948/139‑1 (MQ=255) tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 2:535786/139‑1 (MQ=255) cTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAg < 1:306022/139‑1 (MQ=255) aGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTc < 1:59539/118‑1 (MQ=255) aGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTc > 2:59539/1‑118 (MQ=255) gAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGt < 1:278721/139‑1 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGgag > 2:46933/1‑74 (MQ=255) cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGgag < 1:46933/74‑1 (MQ=255) gTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAgg > 2:1117944/1‑101 (MQ=255) gTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAgg < 1:1117944/101‑1 (MQ=255) cGTTCTGCCCGCGAAGCCGTTTGGCCCGTCTGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGCGCGGTGCTGCCTCTCTCGTTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGa > 1:714896/1‑139 (MQ=255) ttCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCctct < 1:1120186/83‑1 (MQ=255) ttCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCctct > 2:1120186/1‑83 (MQ=255) ttCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt > 1:1218571/1‑97 (MQ=255) ttCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt < 2:1218571/97‑1 (MQ=255) cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc > 1:438732/1‑77 (MQ=255) cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 2:438732/77‑1 (MQ=255) cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAttt < 2:919919/117‑1 (MQ=255) cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAttt > 1:919919/1‑117 (MQ=255) gCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCctc > 2:607679/1‑69 (MQ=255) gCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCctc < 1:607679/69‑1 (MQ=255) ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCt > 1:1084877/1‑131 (MQ=255) ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCt < 2:1084877/131‑1 (MQ=255) ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgt > 2:1031262/1‑139 (MQ=255) ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgt > 1:651248/1‑139 (MQ=255) gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtg > 2:1102971/1‑139 (MQ=255) gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtg > 1:1111147/1‑139 (MQ=255) cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCtata > 1:585559/1‑108 (MQ=255) cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCtata < 2:585559/108‑1 (MQ=255) cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtgt > 1:816476/1‑139 (MQ=255) ggAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCt < 2:651248/139‑1 (MQ=255) aCGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGc > 2:517035/1‑139 (MQ=255) aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt < 1:1185156/52‑1 (MQ=255) aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt > 2:1185156/1‑52 (MQ=255) aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTatat < 1:928129/58‑1 (MQ=255) aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTatat > 2:928129/1‑58 (MQ=255) agaagaGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACa > 2:975109/1‑139 (MQ=255) gaagaGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACAt < 2:994219/139‑1 (MQ=255) agagTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCa < 2:1111147/139‑1 (MQ=255) agagTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCa < 2:816476/139‑1 (MQ=255) agTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAtt > 1:806508/1‑47 (MQ=255) agTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAtt < 2:806508/47‑1 (MQ=255) | AAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCA > NC_000913/4078306‑4078570 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |