Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,078,438 |
G→A |
*330* (TAG→TAA) |
yiiD → |
GNAT family putative N‑acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,078,438 | 0 | G | A | 100.0%
| 98.6
/ NA
| 32 | *330* (TAG→TAA) | yiiD | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/15); total (17/15) |
CAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCT > NC_000913/4078319‑4078560
|
cAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtg > 1:304155/1‑139 (MQ=255)
cTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAg < 2:663703/139‑1 (MQ=255)
tGAAGGCTCGTATATCGTTCTGCCCGAGAAGCCATTTGGCCCGTAAGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGTCTCTCTCATTCAGCTCATTTATATTAGTCCTTGGCTATTTCgg < 2:544324/139‑1 (MQ=255)
cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCgg < 1:30908/133‑1 (MQ=255)
cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCgg > 2:30908/1‑133 (MQ=255)
cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat > 1:723777/1‑139 (MQ=255)
cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgc < 2:319972/85‑1 (MQ=255)
cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgc > 1:319972/1‑85 (MQ=255)
ttCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc > 2:336727/1‑79 (MQ=255)
ttCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 1:336727/79‑1 (MQ=255)
cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc > 2:363676/1‑77 (MQ=255)
cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 1:363676/77‑1 (MQ=255)
cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATAtt < 1:817056/102‑1 (MQ=255)
cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATAtt > 2:817056/1‑102 (MQ=255)
cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAActc > 2:364856/1‑139 (MQ=255)
tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAActct > 2:452934/1‑139 (MQ=255)
cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc > 2:75274/1‑74 (MQ=255)
cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 1:75274/74‑1 (MQ=255)
ggCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTc < 2:266508/75‑1 (MQ=255)
ggCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTc > 1:266508/1‑75 (MQ=255)
cccGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGt < 2:53426/139‑1 (MQ=255)
agGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATAc > 2:770279/1‑139 (MQ=255)
gggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCgg < 2:250043/88‑1 (MQ=255)
gggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCgg > 1:250043/1‑88 (MQ=255)
ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc < 2:47427/44‑1 (MQ=255)
ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc > 1:47427/1‑44 (MQ=255)
cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCCGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCTATACgtgt > 2:201213/1‑139 (MQ=255)
cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAt > 1:243294/1‑80 (MQ=255)
cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAt < 2:243294/80‑1 (MQ=255)
ggAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCt < 1:551816/139‑1 (MQ=255)
aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAtt < 2:343501/53‑1 (MQ=255)
aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAtt > 1:343501/1‑53 (MQ=255)
|
CAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCT > NC_000913/4078319‑4078560
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
