Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,078,438	G→A	330 (TAG→TAA)	yiiD →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,078,438	0	G	A	100.0%	150.8 / NA	47	330 (TAG→TAA)	yiiD	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/25); total (22/25)

ACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTAC  >  NC_000913/4078303‑4078566

aCGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTg                                                                                                                               >  2:1159348/1‑139 (MQ=255)

     aaGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGga                                                                                                                          <  1:1323693/139‑1 (MQ=255)

                  gATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGAAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgct                                                                                                             >  1:676175/1‑139 (MQ=255)

                    tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                           <  1:1159348/139‑1 (MQ=255)

                    tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                           <  1:518256/139‑1 (MQ=255)

                    tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                           <  2:676175/139‑1 (MQ=255)

                        ggTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCctct                                                                                                       >  1:1278146/1‑139 (MQ=255)

                        ggTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCctct                                                                                                       >  1:192956/1‑139 (MQ=255)

                         gTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCctctc                                                                                                      >  2:1191124/1‑139 (MQ=255)

                             aaaTCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCAt                                                                                                  >  1:485164/1‑139 (MQ=255)

                             aaaTCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCAt                                                                                                  >  1:459302/1‑139 (MQ=255)

                              aaTCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCAtt                                                                                                 <  2:459302/139‑1 (MQ=255)

                              aaTCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCAtt                                                                                                 <  2:485164/139‑1 (MQ=255)

                              aaTCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTGCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCAtt                                                                                                 <  1:207905/139‑1 (MQ=255)

                                  tttGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAgg                                                                                             <  1:175402/139‑1 (MQ=255)

                                  tttGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAgg                                                                                             <  1:722871/139‑1 (MQ=255)

                                                 ccGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTc                                                                              <  1:1318442/139‑1 (MQ=255)

                                                       gCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGc                                                                        >  2:144044/1‑139 (MQ=255)

                                                            gtTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAttt                                                                   <  2:573668/139‑1 (MQ=255)

                                                               tGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt                                                                                         >  2:758778/1‑114 (MQ=255)

                                                               tGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt                                                                                         <  1:758778/114‑1 (MQ=255)

                                                               tGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCgg                                                                <  1:1191124/139‑1 (MQ=255)

                                                                    gCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTtata                                                           <  2:1278146/139‑1 (MQ=255)

                                                                     cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTTTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat                                                          <  1:219287/139‑1 (MQ=255)

                                                                     cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat                                                          >  1:546652/1‑139 (MQ=255)

                                                                     cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCCCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat                                                          <  2:762543/139‑1 (MQ=255)

                                                                       cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCg                                                                                                                  <  2:642781/81‑1 (MQ=255)

                                                                       cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCg                                                                                                                  >  1:642781/1‑81 (MQ=255)

                                                                       cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTa                                                        >  1:1148448/1‑139 (MQ=255)

                                                                                ttCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt                                                                                         >  1:1029479/1‑97 (MQ=255)

                                                                                ttCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt                                                                                         <  2:1029479/97‑1 (MQ=255)

                                                                                   tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAgggg                                                                                                                    <  2:865089/67‑1 (MQ=255)

                                                                                   tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAgggg                                                                                                                    >  1:865089/1‑67 (MQ=255)

                                                                                     cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                           <  2:449875/74‑1 (MQ=255)

                                                                                     cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                           >  1:449875/1‑74 (MQ=255)

                                                                                     cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCtat                                          >  2:1348570/1‑139 (MQ=255)

                                                                                        gcgAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTa                                                                                 >  1:649107/1‑97 (MQ=255)

                                                                                        gcgAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTa                                                                                 <  2:649107/97‑1 (MQ=255)

                                                                                                                    gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTgg                                                                         <  1:582247/77‑1 (MQ=255)

                                                                                                                    gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTgg                                                                         >  2:582247/1‑77 (MQ=255)

                                                                                                                    gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtg           >  2:505917/1‑139 (MQ=255)

                                                                                                                     cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCAtt                                                                                                 <  1:1366079/52‑1 (MQ=255)

                                                                                                                     cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCAtt                                                                                                 >  2:1366079/1‑52 (MQ=255)

                                                                                                                     cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtgt          <  1:505917/139‑1 (MQ=255)

                                                                                                                             aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCctct                                                                                                       >  1:992354/1‑38 (MQ=255)

                                                                                                                             aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCctct                                                                                                       <  2:992354/38‑1 (MQ=255)

                                                                                                                             aagaagAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTAc  >  2:507384/1‑139 (MQ=255)

ACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTAC  >  NC_000913/4078303‑4078566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A