Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,078,438	G→A	330 (TAG→TAA)	yiiD →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,078,438	0	G	A	100.0%	147.0 / NA	46	330 (TAG→TAA)	yiiD	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/30); total (16/30)

GACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCA  >  NC_000913/4078302‑4078570

gACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACt                                                                                                                                     >  1:601537/1‑139 (MQ=255)

  cGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGa                                                                                                                                   <  1:324000/139‑1 (MQ=255)

           cGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAggg                                                                                                                          <  2:160695/139‑1 (MQ=255)

               tGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCgg                                                                                                                      >  2:71019/1‑139 (MQ=255)

               tGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCgg                                                                                                                      >  2:634189/1‑139 (MQ=255)

                 cAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtg                                                                                                                    <  2:818106/139‑1 (MQ=255)

                 cAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtg                                                                                                                    <  2:54776/139‑1 (MQ=255)

                    aTGCAGGTCGAATTCTTTGGCGACGGGACGCCGGGTGCAGTGTTTGAAGGCCCTTTTATCTTTCTGCCCCCGAGGCCATTTGGCCCGTATGAAGGGGGCGGGAACGAAGAA‑AGGAACTGATGGAGAGGGGCGGtgctgc                                                                                                                <  2:446291/139‑1 (MQ=255)

                     tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                                <  1:829278/139‑1 (MQ=255)

                         ggTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCct‑ct                                                                                                           <  2:896749/139‑1 (MQ=255)

                         ggTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCct‑ct                                                                                                           >  2:380969/1‑139 (MQ=255)

                               aaTCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCAtt                                                                                                     <  1:380969/139‑1 (MQ=255)

                                   tttGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAgg                                                                                                 <  2:828201/139‑1 (MQ=255)

                                   tttGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAgg                                                                                                 <  1:278653/139‑1 (MQ=255)

                                   tttGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAgg                                                                                                 <  2:1043294/139‑1 (MQ=255)

                                     tGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTCCTGCCCGCGAAGCCATTGGGCCCGTATGAAGAGGGCGGGAACGAAGAAGGGTGACTGATGTAGAGGGGCGGTGCTGACT‑CTCTCATTCAGGTc                                                                                               <  2:271054/139‑1 (MQ=255)

                                       gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             <  2:341879/139‑1 (MQ=255)

                                       gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             <  1:805480/139‑1 (MQ=255)

                                       gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             <  1:834107/139‑1 (MQ=255)

                                       gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             <  2:187998/139‑1 (MQ=255)

                                       gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             <  1:414027/139‑1 (MQ=255)

                                        cgacgaGACGCCGGGTGCAGTGTTTGAATGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAtt                                                                                            <  1:634189/139‑1 (MQ=255)

                                            gagaCGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTtata                                                                                        <  1:581883/139‑1 (MQ=255)

                                                            tgtTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAtt                                                                        <  2:601537/139‑1 (MQ=255)

                                                                 gAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTcc                                                                                 >  1:544920/1‑125 (MQ=255)

                                                                 gAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTcc                                                                                 <  2:544920/125‑1 (MQ=255)

                                                                        cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             >  1:417236/1‑106 (MQ=255)

                                                                        cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             <  2:417236/106‑1 (MQ=255)

                                                                        cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTa                                                            <  2:794426/139‑1 (MQ=255)

                                                                                   cTGCCCGCGAAGCCATTTGGCCCGTATGGAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAActc                                                 >  2:322639/1‑139 (MQ=255)

                                                                                   cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             >  1:1012496/1‑95 (MQ=255)

                                                                                   cTGCCCGCGAAGCCATTTGGCCCGGATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCAt                                                                                             <  2:1012496/95‑1 (MQ=255)

                                                                                    tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAActct                                                >  2:1016705/1‑139 (MQ=255)

                                                                                      cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCtat                                              >  2:391581/1‑139 (MQ=255)

                                                                                          cgAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAAt                                          >  1:877669/1‑139 (MQ=255)

                                                                                                       cccGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                                >  2:709411/1‑57 (MQ=255)

                                                                                                       cccGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                                <  1:709411/57‑1 (MQ=255)

                                                                                                            aTGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCt                        <  2:877669/139‑1 (MQ=255)

                                                                                                             tGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTc                       >  1:95051/1‑139 (MQ=255)

                                                                                                             tGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTc                       <  1:71019/139‑1 (MQ=255)

                                                                                                                ggaggcggggAACAAAGAAGAGTAATTATTGGGAAGGGGC‑GTTCTGCCTCCTTTATTTAAGGTCATTT‑TATTAGTCCTTTGGTTATTCGGTTAATTTTAAAAAGAAATCTTAAAATTTTTATTCTTCGTTGGCTCCATa                   <  1:974730/132‑1 (MQ=255)

                                                                                                                    ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCgg                                                                                                                      <  2:439504/38‑1 (MQ=255)

                                                                                                                    ggCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCgg                                                                                                                      >  1:439504/1‑38 (MQ=255)

                                                                                                                     gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtg               >  2:340512/1‑139 (MQ=255)

                                                                                                                     gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtg               >  2:815777/1‑139 (MQ=255)

                                                                                                                                  agagTAACTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCa  <  1:1016705/139‑1 (MQ=255)

GACGAAAAGCACGGGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCT‑CTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTACATCA  >  NC_000913/4078302‑4078570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A