Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 771 44.8 2153074 94.1% 2026042 109.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,468,106 T→C A84A (GCT→GCC)  gtrA → CPS‑53 (KpLE1) prophage, bactoprenol‑linked glucose translocase/flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,468,1060TC100.0% 215.7 / NA 61A84A (GCT→GCC) gtrACPS‑53 (KpLE1) prophage, bactoprenol‑linked glucose translocase/flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/33);  total (28/33)

TGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCTGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCT  >  NC_000913/2467983‑2468241
                                                                                                                           |                                                                                                                                       
tgtgGCTGTGAGCTTTAGCTTCTCCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCa                                                                                                                          <  1:210512/139‑1 (MQ=255)
  tgGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACt                                                                                                                        <  2:85822/139‑1 (MQ=255)
        tgAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccccc                                                                                                                  <  1:911450/139‑1 (MQ=255)
                aGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATaa                                                                                                          >  1:971520/1‑139 (MQ=255)
                aGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATaa                                                                                                          <  1:327624/139‑1 (MQ=255)
                aGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATaa                                                                                                          <  1:208657/139‑1 (MQ=255)
                    tcttcGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAActct                                                                                                      >  1:1047017/1‑139 (MQ=255)
                      ttcGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTg                                                                                                    >  2:736777/1‑139 (MQ=255)
                           gAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCAcc                                                                                               >  1:865034/1‑139 (MQ=255)
                                      tCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCAt                                                                                    <  2:915130/139‑1 (MQ=255)
                                               aGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCACACTTccc                                                                                                                    >  2:813921/1‑98 (MQ=255)
                                               aGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCACACTTccc                                                                                                                    <  1:813921/98‑1 (MQ=255)
                                                        cTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCAc                                                                                                                         >  2:570604/1‑84 (MQ=255)
                                                        cTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCAc                                                                                                                         <  1:570604/84‑1 (MQ=255)
                                                                 tGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGgtgt                                                                         <  1:130152/123‑1 (MQ=255)
                                                                 tGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGgtgt                                                                         >  2:130152/1‑123 (MQ=255)
                                                                 tGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTc                                                         >  1:749122/1‑139 (MQ=255)
                                                                      tACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGt                                                    <  2:865034/139‑1 (MQ=255)
                                                                        cATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTc                                                  >  1:430207/1‑139 (MQ=255)
                                                                         aTGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                                 <  2:933428/139‑1 (MQ=255)
                                                                         aTGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                                 <  2:1047017/139‑1 (MQ=255)
                                                                          tGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAt                                                <  2:971520/139‑1 (MQ=255)
                                                                           gCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                               <  2:749122/139‑1 (MQ=255)
                                                                           gCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                               <  1:629789/139‑1 (MQ=255)
                                                                             tataTGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAActc                                                                                                       <  1:54983/81‑1 (MQ=255)
                                                                             tataTGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAActc                                                                                                       >  2:54983/1‑81 (MQ=255)
                                                                                    tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCa                                                                                                                          >  1:146780/1‑55 (MQ=255)
                                                                                    tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCa                                                                                                                          <  2:146780/55‑1 (MQ=255)
                                                                                    tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                               <  1:654755/130‑1 (MQ=255)
                                                                                    tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                               >  2:654755/1‑130 (MQ=255)
                                                                                    tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAgg                                      >  1:786946/1‑139 (MQ=255)
                                                                                     gggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTc                                                         >  1:874076/1‑119 (MQ=255)
                                                                                     gggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTc                                                         <  2:874076/119‑1 (MQ=255)
                                                                                      ggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGt                                                               <  2:242246/112‑1 (MQ=255)
                                                                                      ggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGt                                                               >  1:242246/1‑112 (MQ=255)
                                                                                       gTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                                 <  1:219769/125‑1 (MQ=255)
                                                                                       gTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                                 >  2:219769/1‑125 (MQ=255)
                                                                                         tCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                                 >  2:972828/1‑123 (MQ=255)
                                                                                         tCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                                 <  1:972828/123‑1 (MQ=255)
                                                                                           aTGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                                     <  1:67271/85‑1 (MQ=255)
                                                                                           aTGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                                     >  2:67271/1‑85 (MQ=255)
                                                                                           aTGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                        >  1:345695/1‑114 (MQ=255)
                                                                                           aTGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                        <  2:345695/114‑1 (MQ=255)
                                                                                                     tGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAg                        <  2:808896/136‑1 (MQ=255)
                                                                                                     tGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAg                        >  1:808896/1‑136 (MQ=255)
                                                                                                       tgTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGatat                    >  2:883399/2‑138 (MQ=255)
                                                                                                       tgTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGatat                    <  1:883399/137‑1 (MQ=255)
                                                                                                       aGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATAtc                   <  2:777918/139‑1 (MQ=255)
                                                                                                       aGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATAtc                   >  2:221344/1‑139 (MQ=255)
                                                                                                        gTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                               <  2:585619/110‑1 (MQ=255)
                                                                                                        gTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                               >  1:585619/1‑110 (MQ=255)
                                                                                                         tGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAt                                                <  1:922135/108‑1 (MQ=255)
                                                                                                         tGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAt                                                >  2:922135/1‑108 (MQ=255)
                                                                                                             aCTGTTGGATGGGCCGCTGATAGATGCGCACTTccccc                                                                                                                  <  2:190681/38‑1 (MQ=255)
                                                                                                             aCTGTTGGATGGGCCGCTGATAGATGCGCACTTccccc                                                                                                                  >  1:190681/1‑38 (MQ=255)
                                                                                                             aCTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGt             <  2:430207/139‑1 (MQ=255)
                                                                                                                 ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                                     >  1:1013872/1‑63 (MQ=255)
                                                                                                                 ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                                     <  2:1013872/63‑1 (MQ=255)
                                                                                                                 ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGtt         >  1:482429/1‑139 (MQ=255)
                                                                                                                        ggCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                               <  1:164494/94‑1 (MQ=255)
                                                                                                                        ggCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCt  >  1:374775/1‑139 (MQ=255)
                                                                                                                           |                                                                                                                                       
TGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCTGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGTCT  >  NC_000913/2467983‑2468241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A