Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 774 49.2 2112398 96.9% 2046913 118.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,468,106 T→C A84A (GCT→GCC)  gtrA → CPS‑53 (KpLE1) prophage, bactoprenol‑linked glucose translocase/flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,468,1060TC100.0% 186.8 / NA 51A84A (GCT→GCC) gtrACPS‑53 (KpLE1) prophage, bactoprenol‑linked glucose translocase/flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/29);  total (22/29)

CGCAGGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCTGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGT  >  NC_000913/2467971‑2468239
                                                                                                                                       |                                                                                                                                     
cGCAGGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGgccgct                                                                                                                                    >  2:552362/1‑139 (MQ=255)
cGCAGGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGgccgct                                                                                                                                    >  1:899515/1‑139 (MQ=255)
 gCAGGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGccgctg                                                                                                                                   >  1:969769/1‑139 (MQ=255)
     gTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAg                                                                                                                               <  2:548891/139‑1 (MQ=255)
        tCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGCCTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATg                                                                                                                            <  2:639151/139‑1 (MQ=255)
          gTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATgcg                                                                                                                          <  2:876487/139‑1 (MQ=255)
          gTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTAGATGGGCCGCTGATAGATgcg                                                                                                                          <  2:685161/139‑1 (MQ=255)
                    tgAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccccc                                                                                                                <  1:548845/139‑1 (MQ=255)
                       gCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCgat                                                                                                             <  2:800412/139‑1 (MQ=255)
                           tAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATa                                                                                                         <  1:507028/139‑1 (MQ=255)
                            aGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccc                                                                                                                  <  1:605129/129‑1 (MQ=255)
                            aGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGCTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccc                                                                                                                  >  2:605129/1‑129 (MQ=255)
                              cttcttCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAAct                                                                                                      <  2:810212/139‑1 (MQ=255)
                               ttcttcGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAActc                                                                                                     <  1:844630/139‑1 (MQ=255)
                                   tcGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGGACTTCCCCCGATGATAACTCTTGt                                                                                                 >  2:229232/1‑139 (MQ=255)
                                           gCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTtct                                                                                         <  1:217794/139‑1 (MQ=255)
                                                   cacaTTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATc                                                                                 >  1:788189/1‑139 (MQ=255)
                                                      aTTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGc                                                                              <  2:658337/139‑1 (MQ=255)
                                                       ttCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGcc                                                                             <  1:835245/139‑1 (MQ=255)
                                                       ttCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGcc                                                                             <  1:229232/139‑1 (MQ=255)
                                                         cAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTTAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTg                                                                           <  1:707699/139‑1 (MQ=255)
                                                                tCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCg                                                                    >  2:297032/1‑139 (MQ=255)
                                                                             tGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTc                                                       >  1:566782/1‑139 (MQ=255)
                                                                               cgcTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCaa                                                     >  2:137839/1‑139 (MQ=255)
                                                                                     aTGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                               <  1:366562/139‑1 (MQ=255)
                                                                                      tGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCACCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAt                                              <  1:552362/139‑1 (MQ=255)
                                                                                       gCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                             <  2:969769/139‑1 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCgat                                                                                                             >  2:68709/1‑66 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCgat                                                                                                             <  1:68709/66‑1 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGCTAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAgg                                    >  1:901650/1‑139 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTtct                                                                                         <  1:836651/86‑1 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTtct                                                                                         >  2:836651/1‑86 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAgg                                    >  1:690300/1‑139 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAgg                                    >  2:582512/1‑139 (MQ=255)
                                                                                                 gggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAggg                                   >  2:117884/1‑139 (MQ=255)
                                                                                                 gggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAggg                                   <  1:117884/139‑1 (MQ=255)
                                                                                                   gTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                             <  2:985663/127‑1 (MQ=255)
                                                                                                   gTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                             >  1:985663/1‑127 (MQ=255)
                                                                                                    ttCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATg                                >  2:897940/1‑139 (MQ=255)
                                                                                                     tCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTtctc                                                                                        >  2:395817/1‑82 (MQ=255)
                                                                                                     tCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTtctc                                                                                        <  1:395817/82‑1 (MQ=255)
                                                                                                     tCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGc                               >  2:742687/1‑139 (MQ=255)
                                                                                                             acacTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCTGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGaa                       >  1:705352/1‑139 (MQ=255)
                                                                                                              cacTGAGTGCTACTGTTGGATGGCCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCGGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAg                      <  2:695649/139‑1 (MQ=255)
                                                                                                                  gAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGatat                  >  2:802764/1‑139 (MQ=255)
                                                                                                                     tGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATAtctc               >  1:601266/1‑139 (MQ=255)
                                                                                                                             ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTc                                                                                                <  1:128394/50‑1 (MQ=255)
                                                                                                                             ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTc                                                                                                >  2:128394/1‑50 (MQ=255)
                                                                                                                              tGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTc      <  2:496920/139‑1 (MQ=255)
                                                                                                                              tGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTc      <  2:566782/139‑1 (MQ=255)
                                                                                                                               ggATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAt                                              <  2:215958/98‑1 (MQ=255)
                                                                                                                               ggATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAt                                              >  1:215958/1‑98 (MQ=255)
                                                                                                                                  tGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGt  >  1:271540/1‑139 (MQ=255)
                                                                                                                                    ggCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGAt                                 <  1:732040/106‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CGCAGGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCTGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGT  >  NC_000913/2467971‑2468239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A