Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I1 R1 759 65.2 3386260 90.7% 3071337 101.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,468,106 T→C A84A (GCT→GCC)  gtrA → CPS‑53 (KpLE1) prophage, bactoprenol‑linked glucose translocase/flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,468,1060TC100.0% 207.1 / NA 58A84A (GCT→GCC) gtrACPS‑53 (KpLE1) prophage, bactoprenol‑linked glucose translocase/flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/32);  total (26/32)

CGCAGGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCTGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGT  >  NC_000913/2467971‑2468239
                                                                                                                                       |                                                                                                                                     
cGCAGGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGgccgct                                                                                                                                    >  2:1017625/1‑139 (MQ=255)
     gTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAg                                                                                                                               <  2:1161936/139‑1 (MQ=255)
        tCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTTCAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATg                                                                                                                            <  2:1213100/139‑1 (MQ=255)
            tgtgGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCa                                                                                                                        <  1:1270035/139‑1 (MQ=255)
             gtgGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCAc                                                                                                                       <  1:1453802/139‑1 (MQ=255)
                    tgAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccccc                                                                                                                >  1:429252/1‑139 (MQ=255)
                    tgAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccccc                                                                                                                <  1:175845/139‑1 (MQ=255)
                        cTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGTCATGGACTACAACGATGCGATACATGCGATATGTTGGGTTCATGGGGAGACGGAGTGGAACTGTTGGATGGGCCGCTGATAGATGCGCACTTccccaaatg                                                                                                            >  2:172938/1‑134 (MQ=255)
                         tttAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCgatga                                                                                                           <  2:783540/139‑1 (MQ=255)
                                tcttcGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGgccgct                                                                                                                                    >  1:1670810/1‑107 (MQ=255)
                                tcttcGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGgccgct                                                                                                                                    <  2:1670810/107‑1 (MQ=255)
                                          tGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATgc                                                                                                                           <  2:199900/106‑1 (MQ=255)
                                          tGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATgc                                                                                                                           >  1:199900/1‑106 (MQ=255)
                                                 ttCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                                   <  2:231780/139‑1 (MQ=255)
                                                 ttCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                                   <  1:662050/139‑1 (MQ=255)
                                                           aGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCa                                                                                               <  2:1051523/117‑1 (MQ=255)
                                                           aGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGgt                                                                         >  1:867453/1‑139 (MQ=255)
                                                           aGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTAGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCa                                                                                               >  1:1051523/1‑117 (MQ=255)
                                                             gCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGgtgt                                                                       <  1:1017625/139‑1 (MQ=255)
                                                                          cGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccc                                                                                                                  >  1:1603721/1‑83 (MQ=255)
                                                                          cGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccc                                                                                                                  <  2:1603721/83‑1 (MQ=255)
                                                                              gcgcTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATg                                                                                                                            <  2:1236037/69‑1 (MQ=255)
                                                                              gcgcTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATg                                                                                                                            >  1:1236037/1‑69 (MQ=255)
                                                                              gcgcTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                      >  2:995995/1‑139 (MQ=255)
                                                                              gcgcTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGAGAAATCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                      <  2:53231/139‑1 (MQ=255)
                                                                                     aTGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                               <  1:172938/139‑1 (MQ=255)
                                                                                       gCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                             <  1:995995/139‑1 (MQ=255)
                                                                                         tataTGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCgatgat                                                                                                          >  1:134665/1‑76 (MQ=255)
                                                                                         tataTGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCgatgat                                                                                                          <  2:134665/76‑1 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAt                                              <  2:541880/129‑1 (MQ=255)
                                                                                                tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAt                                              >  1:541880/1‑129 (MQ=255)
                                                                                                 gggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCgatgat                                                                                                          >  2:531528/1‑68 (MQ=255)
                                                                                                 gggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCgatgat                                                                                                          <  1:531528/68‑1 (MQ=255)
                                                                                                  ggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGa                                  >  1:1043589/1‑139 (MQ=255)
                                                                                                  ggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGa                                  >  2:876575/1‑139 (MQ=255)
                                                                                                      cATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                      <  1:162347/115‑1 (MQ=255)
                                                                                                      cATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                      >  2:162347/1‑115 (MQ=255)
                                                                                                        tGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATATCTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGaa                            >  1:265746/1‑139 (MQ=255)
                                                                                                        tGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGaa                            <  2:1236780/139‑1 (MQ=255)
                                                                                                           ggACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATg                         <  2:429252/139‑1 (MQ=255)
                                                                                                              cacTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                             <  2:1557860/116‑1 (MQ=255)
                                                                                                              cacTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                             >  1:1557860/1‑116 (MQ=255)
                                                                                                                cTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGat                    <  2:867453/139‑1 (MQ=255)
                                                                                                                cTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGACTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGat                    <  2:1043589/139‑1 (MQ=255)
                                                                                                                 tGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCa                                                                                                                        >  2:787868/1‑38 (MQ=38)
                                                                                                                 tGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCa                                                                                                                        <  1:787868/38‑1 (MQ=38)
                                                                                                                   aGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCt                                                           <  1:596752/97‑1 (MQ=255)
                                                                                                                   aGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCt                                                           >  2:596752/1‑97 (MQ=255)
                                                                                                                   aGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGat                    <  1:1349472/136‑1 (MQ=255)
                                                                                                                   aGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGat                    >  2:1349472/1‑136 (MQ=255)
                                                                                                                     tGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGt                                                                  <  2:1231348/88‑1 (MQ=255)
                                                                                                                     tGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGt                                                                  >  1:1231348/1‑88 (MQ=255)
                                                                                                                           tGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                      <  2:300159/94‑1 (MQ=255)
                                                                                                                           tGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                      >  1:300159/1‑94 (MQ=255)
                                                                                                                             ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGtt       >  1:303907/1‑139 (MQ=255)
                                                                                                                             ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGtt       >  1:652632/1‑139 (MQ=255)
                                                                                                                               ggATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTg            <  1:186974/132‑1 (MQ=255)
                                                                                                                               ggATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTg            >  2:186974/1‑132 (MQ=255)
                                                                                                                                 aTGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                             >  2:839886/1‑97 (MQ=255)
                                                                                                                                 aTGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCAtt                                             <  1:839886/97‑1 (MQ=255)
                                                                                                                                  tGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGt  >  1:73773/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CGCAGGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCTGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTCCTGT  >  NC_000913/2467971‑2468239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A