Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I1 R1 776 60.0 3074904 90.1% 2770488 104.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,468,106 T→C A84A (GCT→GCC)  gtrA → CPS‑53 (KpLE1) prophage, bactoprenol‑linked glucose translocase/flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,468,1060TC100.0% 181.7 / NA 52A84A (GCT→GCC) gtrACPS‑53 (KpLE1) prophage, bactoprenol‑linked glucose translocase/flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/30);  total (22/30)

GGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCTGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTC  >  NC_000913/2467975‑2468235
                                                                                                                                   |                                                                                                                                 
ggTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATa                                                                                                                            >  1:505456/1‑139 (MQ=255)
      gTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATgcg                                                                                                                      <  2:1220779/139‑1 (MQ=255)
              tgtgAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccc                                                                                                              <  2:505456/139‑1 (MQ=255)
               gtgAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTcccc                                                                                                             <  2:1049034/139‑1 (MQ=255)
                tgAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTccccc                                                                                                            >  1:1176512/1‑139 (MQ=255)
                       tAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACTATGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATa                                                                                                     >  2:379016/1‑139 (MQ=255)
                                cgcgAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTc                                                                                            <  1:379016/139‑1 (MQ=255)
                                        cAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTtctc                                                                                    <  1:216333/139‑1 (MQ=255)
                                             ttCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                               <  1:808141/139‑1 (MQ=255)
                                             ttCACACTCAAGGCATTGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAAATCTTGTCACCTTCTCCCCCa                                                                               <  1:1043960/139‑1 (MQ=255)
                                                  aTTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGc                                                                          <  2:1176512/139‑1 (MQ=255)
                                                          cATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGtgtg                                                                  <  2:412073/139‑1 (MQ=255)
                                                              gACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGt                                                              <  1:1323273/139‑1 (MQ=255)
                                                                          gcgcTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCa                                                  <  2:949159/139‑1 (MQ=255)
                                                                           cgcTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTTCGGTTTCGTCTATTCaa                                                 >  1:864775/1‑139 (MQ=255)
                                                                           cgcTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCaa                                                 >  1:162507/1‑139 (MQ=255)
                                                                              tACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGcc                                                                         >  2:1406334/1‑112 (MQ=255)
                                                                              tACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGcc                                                                         <  1:1406334/112‑1 (MQ=255)
                                                                                 aTGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                           <  1:594547/139‑1 (MQ=255)
                                                                                     tataTGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                               >  2:564776/1‑99 (MQ=255)
                                                                                     tataTGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                               <  1:564776/99‑1 (MQ=255)
                                                                                     tataTGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTc                                                   <  1:1092365/127‑1 (MQ=255)
                                                                                     tataTGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTc                                                   >  2:1092365/1‑127 (MQ=255)
                                                                                          gTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTCTTCAAAGTTCATTGTCTTTa                                  >  2:34961/1‑139 (MQ=255)
                                                                                            tGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAgg                                >  2:431000/1‑139 (MQ=255)
                                                                                              ggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATg                                                                                                                        <  1:620250/49‑1 (MQ=255)
                                                                                              ggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATg                                                                                                                        >  2:620250/1‑49 (MQ=255)
                                                                                              ggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                           <  1:19332/126‑1 (MQ=255)
                                                                                              ggTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCa                                           >  2:19332/1‑126 (MQ=255)
                                                                                                 tCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                               <  1:673566/87‑1 (MQ=255)
                                                                                                 tCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCa                                                                               >  2:673566/1‑87 (MQ=255)
                                                                                                 tCATGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGc                           >  2:174664/1‑139 (MQ=255)
                                                                                                   aTGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGCTAGATGCGCACTTccccc                                                                                                            <  1:1010811/56‑1 (MQ=255)
                                                                                                   aTGGGGACACTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTcccgc                                                                                                            >  2:1010811/1‑54 (MQ=255)
                                                                                                          cacTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGc                                                                                 >  2:266817/1‑76 (MQ=255)
                                                                                                          cacTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGc                                                                                 <  1:266817/76‑1 (MQ=255)
                                                                                                          cacTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGtgtg                                                                  <  2:1378032/91‑1 (MQ=255)
                                                                                                          cacTGAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGtgtg                                                                  >  1:1378032/1‑91 (MQ=255)
                                                                                                              gAGTGCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGatat              <  1:174664/139‑1 (MQ=255)
                                                                                                                  gCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGtgtg                                                                  >  2:1261029/1‑83 (MQ=255)
                                                                                                                  gCTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGtgtg                                                                  <  1:1261029/83‑1 (MQ=255)
                                                                                                                   cTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGt                                                              <  2:211348/86‑1 (MQ=255)
                                                                                                                   cTACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGt                                                              >  1:211348/1‑86 (MQ=255)
                                                                                                                    tACTGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTg        <  2:162507/139‑1 (MQ=255)
                                                                                                                       tGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGt                                                              >  2:356416/1‑82 (MQ=255)
                                                                                                                       tGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGt                                                              <  1:356416/82‑1 (MQ=255)
                                                                                                                       tGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAAt                      <  1:1096254/122‑1 (MQ=255)
                                                                                                                       tGTTGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGAGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAAt                      >  2:1096254/1‑122 (MQ=255)
                                                                                                                         ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATAtc             <  2:1454427/129‑1 (MQ=255)
                                                                                                                         ttGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATAtc             >  1:1454427/1‑129 (MQ=255)
                                                                                                                          tGGATGGGCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTc  <  1:687160/139‑1 (MQ=255)
                                                                                                                                ggCCGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTc                                                                                            <  1:486302/43‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
GGTTTCGTTGTGGCTGTGAGCTTTAGCTTCTTCGCGAATGCAAAATTCACATTCAAGGCATCGACTACAACGATGCGCTACATGCTATATGTTGGGTTCATGGGGACACTGAGTGCTACTGTTGGATGGGCTGCTGATAGATGCGCACTTCCCCCGATGATAACTCTTGTCACCTTCTCCGCCATCAGCCTGGTGTGCGGTTTCGTCTATTCAAAGTTCATTGTCTTTAGGGATGCGAAATGAAGATATCTCTTGTAGTTC  >  NC_000913/2467975‑2468235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A