Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I1 R1 776 60.0 3074904 90.1% 2770488 104.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,600,609 (C)5→6 coding (132/471 nt) bcp → peroxiredoxin, thiol peroxidase, thioredoxin‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,600,6041.C100.0% 181.7 / NA 52T43P (ACC→CCC) bcpperoxiredoxin, thiol peroxidase, thioredoxin‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/26);  total (26/26)

AGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAG  >  NC_000913/2600469‑2600732
                                                                                                                                        |                                                                                                                                
aGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                               <  2:599701/139‑1 (MQ=255)
aGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                               >  1:656141/1‑139 (MQ=255)
          tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      >  1:589142/1‑139 (MQ=255)
          tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      >  1:578898/1‑139 (MQ=255)
          tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      >  2:445884/1‑139 (MQ=255)
          tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      <  2:100703/139‑1 (MQ=255)
            aaTCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTAcc                                                                                                                    >  2:395384/1‑139 (MQ=255)
            aaTCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTAcc                                                                                                                    >  2:771116/1‑139 (MQ=255)
                cACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      <  2:1340436/133‑1 (MQ=255)
                cACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      >  1:1340436/1‑133 (MQ=255)
                 aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACa                                                                                                               >  2:252417/1‑139 (MQ=255)
                 aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATAACCCCCCGGCTGTa                                                                                                                      <  1:655037/132‑1 (MQ=255)
                 aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATAACCCCCCGGCTGTa                                                                                                                      >  2:655037/1‑132 (MQ=255)
                             tGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      <  1:1498042/120‑1 (MQ=255)
                             tGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      >  2:1498042/1‑120 (MQ=255)
                                  tCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTAcgcg                                                                                              <  2:1464821/139‑1 (MQ=255)
                                   cGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                             <  2:641287/139‑1 (MQ=255)
                                   cGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                             <  1:395384/139‑1 (MQ=255)
                                     cACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccc                                                                                                                             <  1:804291/104‑1 (MQ=255)
                                     cACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccc                                                                                                                             >  2:804291/1‑104 (MQ=255)
                                       ccGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                                 <  1:410476/115‑1 (MQ=255)
                                       ccGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                                 >  2:410476/1‑115 (MQ=255)
                                          aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      <  2:1403771/107‑1 (MQ=255)
                                          aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      >  1:1403771/1‑107 (MQ=255)
                                           aaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATgg                                                                                     <  2:985425/139‑1 (MQ=255)
                                           aaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATgg                                                                                     <  2:589142/139‑1 (MQ=255)
                                             tttAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                   <  2:1510737/139‑1 (MQ=255)
                                                          aTCAAGACGGAGAACAAGTTAATTTGACTGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                               >  1:949727/1‑81 (MQ=255)
                                                          aTCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                               <  2:949727/81‑1 (MQ=255)
                                                                    agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTg                                                                                                                        >  2:834298/1‑79 (MQ=255)
                                                                    agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTg                                                                                                                        <  1:834298/79‑1 (MQ=255)
                                                                      aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg                                                          <  2:656141/139‑1 (MQ=255)
                                                                      aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg                                                          <  1:445884/139‑1 (MQ=255)
                                                                        cAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAATAGCGGGCGTTGAtgtg                                                        <  1:89550/139‑1 (MQ=255)
                                                                        cAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg                                                        <  1:771116/139‑1 (MQ=255)
                                                                           gTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      >  1:287259/1‑74 (MQ=255)
                                                                           gTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                      <  2:287259/74‑1 (MQ=255)
                                                                                tttGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTAt                                                >  2:581753/1‑139 (MQ=255)
                                                                                   gACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACa                                                                                                               >  1:239928/1‑73 (MQ=255)
                                                                                   gACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACa                                                                                                               <  2:239928/73‑1 (MQ=255)
                                                                                    aCCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGc                                            >  1:1212391/1‑139 (MQ=255)
                                                                                            ccAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATaa                                    <  2:1212391/139‑1 (MQ=255)
                                                                                              aGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAAc                                  <  2:1019937/139‑1 (MQ=255)
                                                                                                   cAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTg                                                             <  1:1494688/107‑1 (MQ=255)
                                                                                                   cAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTg                                                             >  2:1494688/1‑107 (MQ=255)
                                                                                                    aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg                                                     >  2:1307339/1‑114 (MQ=255)
                                                                                                    aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg                                                     <  1:1307339/114‑1 (MQ=255)
                                                                                                    aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaa                            >  2:610701/1‑139 (MQ=255)
                                                                                                    aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaa                            >  1:390735/1‑139 (MQ=255)
                                                                                                      cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg                                                        <  2:333773/109‑1 (MQ=255)
                                                                                                      cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg                                                        >  1:333773/1‑109 (MQ=255)
                                                                                                      cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa                          >  2:1188355/1‑139 (MQ=255)
                                                                                                      cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                          >  2:1499657/1‑39 (MQ=255)
                                                                                                      cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                          <  1:1499657/42‑4 (MQ=255)
                                                                                                        tgtTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGc                                            <  2:1409057/119‑1 (MQ=255)
                                                                                                        tgtTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGc                                            >  1:1409057/1‑119 (MQ=255)
                                                                                                                    tttCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGc            >  2:358014/1‑139 (MQ=255)
                                                                                                                         aCCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGaaa       >  2:895274/1‑139 (MQ=255)
                                                                                                                              aaaGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAagag  <  1:610701/139‑1 (MQ=255)
                                                                                                                              aaaGCCATGACCCCCCGGCTGTACCCTCCAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAACCCCGAAAAAATCTCCCGTTTTGCGGAAAAagat  >  1:1499257/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                
AGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAG  >  NC_000913/2600469‑2600732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A