Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,600,609	(C)_5→6	coding (132/471 nt)	bcp →	peroxiredoxin, thiol peroxidase, thioredoxin‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,600,604	1	.	C	100.0%	155.1 / NA	43	T43P (ACC→CCC)	bcp	peroxiredoxin, thiol peroxidase, thioredoxin‑dependent
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (21/22); total (21/22)

GGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAGCTGCT  >  NC_000913/2600467‑2600737

ggAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑c                                                                                                                                      >  1:4477/1‑139 (MQ=255)

       aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                               >  2:891448/1‑136 (MQ=255)

       aGTAATGAATCCACTGAAAGCCGGAGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccccccgg                                                                                                                               >  1:96494/1‑136 (MQ=255)

            tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTa                                                                                                                           <  2:797309/139‑1 (MQ=255)

                 ccACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCAACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTa                                                                                                                      <  2:473128/139‑1 (MQ=255)

                       aaaGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGcc                                                                                                                >  1:910136/1‑139 (MQ=255)

                             ggTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGc                                                                                                          <  2:910136/139‑1 (MQ=255)

                              gTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCt                                                                                                         <  2:897453/139‑1 (MQ=255)

                                 atatCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTAc                                                                                                      >  2:309917/1‑139 (MQ=255)

                                     cGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                                  <  1:836550/139‑1 (MQ=255)

                                     cGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa                                                                                                  <  1:8498/139‑1 (MQ=255)

                                       cACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATa                                                                                                >  1:702310/1‑139 (MQ=255)

                                        aCCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATaa                                                                                               >  1:738916/1‑139 (MQ=255)

                                         ccGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTCTATTTCTACCCGAAAGCCATTACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAAc                                                                                              >  2:213925/1‑139 (MQ=255)

                                            aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACGGGCCTGCGGCTTACGCGATAACATg                                                                                           >  1:466560/1‑139 (MQ=255)

                                            aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCg                                                                                                            >  2:318442/1‑122 (MQ=255)

                                            aaaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCg                                                                                                            <  1:318442/122‑1 (MQ=255)

                                             aaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTAc                                                                                                                     <  2:982398/112‑1 (MQ=255)

                                             aaTTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTAc                                                                                                                     >  1:982398/1‑112 (MQ=255)

                                               tttAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                        <  2:4477/139‑1 (MQ=255)

                                               tttAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                        <  1:309917/139‑1 (MQ=255)

                                               tttAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                        <  1:942791/139‑1 (MQ=255)

                                               tttAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt                                                                                        <  1:1013320/139‑1 (MQ=255)

                                                       gCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGaa                                                                                >  2:523342/1‑139 (MQ=255)

                                                                     gagaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTg                                                                  <  2:702310/139‑1 (MQ=255)

                                                                        aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg                                                               >  2:444150/1‑139 (MQ=255)

                                                                           gtgTTAATGTGACCGACTTCCCGGGCCAGCGTGTTCTTGTTCATTTCTACCCGACGGCCATGACCCCCCGGGTGTACCGTACAGGCCTGCGGATTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGc                                                            <  2:130048/137‑1 (MQ=255)

                                                                                          aCTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCg                                             >  1:5057/1‑139 (MQ=255)

                                                                                                      aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTAcg                                                                                                     <  2:322373/71‑1 (MQ=255)

                                                                                                      aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTAcg                                                                                                     >  1:322373/1‑71 (MQ=255)

                                                                                                      aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaa                                 >  2:682416/1‑139 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGa                                                                                      >  2:319372/1‑84 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGa                                                                                      <  1:319372/84‑1 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg                                                          >  2:298769/1‑112 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg                                                          <  1:298769/112‑1 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa                               >  2:398189/1‑139 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa                               >  1:421589/1‑139 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa                               >  1:831154/1‑139 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                                    <  1:161305/37‑1 (MQ=255)

                                                                                                        cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc                                                                                                                                    >  2:161305/1‑37 (MQ=255)

                                                                                                                  tttATTTCTACCCGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGtt                     <  1:891448/139‑1 (MQ=255)

                                                                                                                              cGAAAGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAag         <  2:421589/139‑1 (MQ=255)

                                                                                                                                aaaGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGc                                                                      >  2:259446/1‑76 (MQ=255)

                                                                                                                                aaaGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGc                                                                      <  1:259446/76‑1 (MQ=255)

                                                                                                                                aaaGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAagag       >  1:1024721/1‑139 (MQ=255)

                                                                                                                                 aaGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCg                                                                                                            >  2:389357/1‑37 (MQ=255)

                                                                                                                                 aaGCCATGACCCCCCGGCTGTACCGTACAGGCCTGCg                                                                                                            <  1:389357/37‑1 (MQ=255)

                                                                                                                                     cATGACCCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAgctgct  <  1:682416/139‑1 (MQ=255)

GGAGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAGCTGCT  >  NC_000913/2600467‑2600737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A