Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,600,609 |
(C)5→6 |
coding (132/471 nt) |
bcp → |
peroxiredoxin, thiol peroxidase, thioredoxin‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,600,604 | 1 | . | C | 98.0%
| 178.4
/ ‑3.4
| 51 | coding (127/471 nt) | bcp | peroxiredoxin, thiol peroxidase, thioredoxin‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (24/26); total (25/26) |
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
AGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAG > NC_000913/2600469‑2600732
|
aGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc > 2:32605/1‑139 (MQ=255)
aGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc < 1:32605/139‑1 (MQ=255)
aGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc < 1:520381/139‑1 (MQ=255)
ttAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cc < 1:972374/139‑1 (MQ=255)
ttAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cc < 2:738523/139‑1 (MQ=255)
aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg > 1:80901/1‑136 (MQ=255)
aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg < 2:14020/139‑4 (MQ=255)
aGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg > 1:628545/1‑136 (MQ=255)
tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTg < 2:275546/137‑1 (MQ=255)
tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTg > 1:275546/1‑137 (MQ=255)
tGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa < 1:2607/139‑1 (MQ=255)
aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGGCTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa > 1:331485/1‑132 (MQ=255)
aCTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGGCTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa < 2:331485/132‑1 (MQ=255)
tGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAgg > 2:372413/1‑139 (MQ=255)
aaaGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGcc < 2:80901/139‑1 (MQ=255)
aGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTg < 2:842001/139‑1 (MQ=255)
ggTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGc > 2:15846/1‑139 (MQ=255)
gTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCt < 2:398111/139‑1 (MQ=255)
gTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCt < 2:628545/139‑1 (MQ=255)
cGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTg > 1:526271/1‑127 (MQ=255)
cGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTg < 2:526271/127‑1 (MQ=255)
cGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGa < 1:397712/139‑1 (MQ=255)
gCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGAt < 1:162751/139‑1 (MQ=255)
gAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACAt > 1:670755/1‑139 (MQ=255)
ttGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTg < 1:1247092/96‑1 (MQ=255)
ttGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTg > 2:1247092/1‑96 (MQ=255)
tGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa > 1:1099848/1‑97 (MQ=255)
tGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa < 2:1099848/97‑1 (MQ=255)
agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa < 2:657056/81‑1 (MQ=255)
agaACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTa > 1:657056/1‑81 (MQ=255)
aaCAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg < 2:670755/139‑1 (MQ=255)
gTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg > 2:850429/1‑139 (MQ=255)
gTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTg < 2:966998/139‑1 (MQ=255)
ccGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCa < 1:427511/139‑1 (MQ=255)
cAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTa > 2:78209/1‑55 (MQ=255)
cAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTa < 1:78209/55‑1 (MQ=255)
cAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGc > 1:230014/1‑67 (MQ=255)
cAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGc < 2:230014/67‑1 (MQ=255)
aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg < 2:514239/111‑1 (MQ=255)
aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg > 1:514239/1‑111 (MQ=255)
aGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaa > 2:485920/1‑139 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATaa > 2:982338/1‑75 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATaa < 1:982338/75‑1 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt < 2:1167955/82‑1 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGAt > 1:1167955/1‑82 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg > 1:692756/1‑107 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtg < 2:692756/107‑1 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAt < 2:877319/106‑1 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAt > 1:877319/1‑106 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTgg > 2:279522/1‑113 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTgg < 1:279522/113‑1 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGaaaaa > 1:796991/1‑139 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg > 1:532558/1‑39 (MQ=255)
cGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑ccc‑cccgg < 2:532558/42‑4 (MQ=255)
agtgtTCTGGTTTATTTCTACCCCAAAGCAATGA‑CCCGCCGACAGTAACCTACAGGCCTGCGGCTTTCGCGATATCATGGATGAGTTGAAAAAAGGAGGCGTTGAAGTGCTTGGTATCCGCACCGATCAACCCGaaaaa > 2:1130485/2‑139 (MQ=255)
ttCTGGTTTATTTCTACCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAActct > 1:405844/1‑139 (MQ=255)
aCCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCg < 1:1183950/43‑1 (MQ=255)
aCCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCg > 2:1183950/1‑43 (MQ=255)
aCCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg > 1:1034179/1‑90 (MQ=255)
aCCCGAAAGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGAtgtg < 2:1034179/90‑1 (MQ=255)
aaaGCCATGACCCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAGAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAagag > 2:1139656/1‑139 (MQ=255)
|
AGTTAAGTAATGAATCCACTGAAAGCCGGTGATATCGCACCGAAATTTAGCTTGCCGGATCAAGACGGAGAACAAGTTAATTTGACCGACTTCCAGGGACAGCGTGTTCTGGTTTATTTCTACCCGAAAGCCATGA‑CCC‑CCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAACCCGAAAAACTCTCCCGTTTTGCGGAAAAAGAG > NC_000913/2600469‑2600732
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
