Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
760 |
58.2 |
3022526 |
90.8% |
2744453 |
102.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,200,454 |
(A)8→7 |
coding (176/3633 nt) |
yehI → |
DUF4132 domain‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,200,447 | 0 | A | . | 100.0%
| 128.1
/ NA
| 32 | coding (169/3633 nt) | yehI | DUF4132 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (19/13); total (19/13) |
ACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGC > NC_000913/2200317‑2200571
|
aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca < 1:1296987/139‑3 (MQ=255)
gCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATc > 2:1399970/1‑139 (MQ=255)
tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCt < 1:901380/96‑1 (MQ=255)
tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCt > 2:901380/1‑96 (MQ=255)
tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCAc > 2:833911/1‑139 (MQ=255)
tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg < 1:833911/139‑1 (MQ=255)
cTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTATCTGGCGTTATGAGAA‑TTTTTCAc < 2:745456/89‑1 (MQ=255)
cTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTATCTGGCGTTATGAGAA‑TTTTTCAc > 1:745456/1‑89 (MQ=255)
ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaaga > 2:882056/1‑52 (MQ=255)
ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaaga < 1:882056/52‑1 (MQ=255)
ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTc > 1:189249/1‑94 (MQ=255)
ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTc < 2:189249/94‑1 (MQ=255)
aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt > 1:1225248/1‑139 (MQ=255)
aTTGGGTGCTGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCCGGCGTTATGAGAA‑TTTTCCACTGGAGTGGCAGTCCGCCATCACGCATGCCATCAACTTGATCGTCGAACACAAACACTCACTcccggccc > 2:1028315/1‑139 (MQ=255)
tGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccgg > 2:1185208/1‑139 (MQ=255)
tGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc < 1:59542/135‑1 (MQ=255)
tGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc > 2:59542/1‑135 (MQ=255)
gggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGa > 1:577706/1‑139 (MQ=255)
gggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGa > 2:64376/1‑139 (MQ=255)
ggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAgg > 1:1036368/1‑93 (MQ=255)
ggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAgg < 2:1036368/93‑1 (MQ=255)
ggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc > 1:692814/1‑139 (MQ=255)
atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCAc > 2:779405/1‑62 (MQ=255)
atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCAc < 1:779405/62‑1 (MQ=255)
atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCa > 2:304073/1‑87 (MQ=255)
atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCa < 1:304073/87‑1 (MQ=255)
tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg < 1:1185208/139‑1 (MQ=255)
gCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTgg < 2:418753/63‑1 (MQ=255)
gCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTgg > 1:418753/1‑63 (MQ=255)
cTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGGTATGAGAATTTTTTAATTGAAGTGCCA‑TCCCGCATCACGCAGGCCATCAACTTGGTTCGCGGACACAAACCCGCAATTCCCGCCCGGGAAATGGCGGCGCt > 1:903382/1‑139 (MQ=255)
aCAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCa > 2:1485606/1‑82 (MQ=255)
aCAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCa < 1:1485606/82‑1 (MQ=255)
cAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGc < 1:64376/139‑1 (MQ=255)
aGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGc < 1:132374/62‑1 (MQ=255)
aGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGc > 2:132374/1‑62 (MQ=255)
agaag‑aAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg < 2:1151841/124‑1 (MQ=255)
agaag‑aAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg > 1:1151841/1‑124 (MQ=255)
|
ACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAA‑TTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGC > NC_000913/2200317‑2200571
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
