Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A6 F1 I2 R1 | 758 | 59.3 | 2864096 | 93.9% | 2689386 | 105.7 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,200,454 | (A)8→7 | coding (176/3633 nt) | yehI → | DUF4132 domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,200,447 | 0 | A | . | 100.0% | 151.7 / NA | 37 | coding (169/3633 nt) | yehI | DUF4132 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (19/18); total (19/18) | |||||||||||
ACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG > NC_000913/2200317‑2200581 | aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca < 1:51966/139‑3 (MQ=255) atatAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGTCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGtt < 2:6274/139‑1 (MQ=255) aaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAAt > 2:521020/1‑139 (MQ=255) ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa < 1:521020/139‑1 (MQ=255) gggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCAc > 2:475551/1‑112 (MQ=255) gggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCAc < 1:475551/112‑1 (MQ=255) cGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa > 1:762705/1‑122 (MQ=255) cGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa < 2:762705/122‑1 (MQ=255) gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc < 2:516180/120‑1 (MQ=255) gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc > 1:516180/1‑120 (MQ=255) tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg > 1:969806/1‑139 (MQ=255) ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa > 2:768764/1‑139 (MQ=255) ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCAATGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATAGGCGAACACaa > 2:1021530/1‑139 (MQ=255) aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTc < 1:125302/76‑1 (MQ=255) aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTc > 2:125302/1‑76 (MQ=255) ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGaa < 1:1064115/68‑1 (MQ=255) ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGaa > 2:1064115/1‑68 (MQ=255) ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCa < 1:258064/106‑1 (MQ=255) ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCa > 2:258064/1‑106 (MQ=255) aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc > 1:294750/1‑139 (MQ=255) tGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc > 1:1367796/1‑130 (MQ=255) tGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc < 2:1367796/130‑1 (MQ=255) gggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt > 1:660068/1‑124 (MQ=255) gggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt < 2:660068/124‑1 (MQ=255) ggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATga > 2:586048/1‑55 (MQ=255) ggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATga < 1:586048/55‑1 (MQ=255) atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCAc > 2:852370/1‑62 (MQ=255) atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCAc < 1:852370/62‑1 (MQ=255) atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc < 2:1248010/86‑1 (MQ=255) atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc > 1:1248010/1‑86 (MQ=255) tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg < 2:294750/139‑1 (MQ=255) tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg < 2:299152/139‑1 (MQ=255) tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg < 1:397000/139‑1 (MQ=255) gCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGgcgc > 2:41173/1‑139 (MQ=255) aGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc > 1:671563/1‑108 (MQ=255) aGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc < 2:671563/108‑1 (MQ=255) cTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc > 2:449637/1‑57 (MQ=255) cTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc < 1:449637/57‑1 (MQ=255) agaag‑aAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCa < 2:1079550/59‑1 (MQ=255) agaag‑aAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCa > 1:1079550/1‑59 (MQ=255) agaag‑aAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATcgcg > 1:166199/1‑139 (MQ=255) | ACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG > NC_000913/2200317‑2200581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |