Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,200,454	(A)_8→7	coding (176/3633 nt)	yehI →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,200,447	0	A	.	100.0%	85.4 / NA	21	coding (169/3633 nt)	yehI	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (10/11); total (10/11)

AACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCG  >  NC_000913/2200309‑2200566

aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTTGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaaga                                                                                                                         >  2:374368/1‑139 (MQ=255)

                              ggCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCCTCCAGAAATTGGGGGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAAttt                                                                                          <  2:314899/139‑1 (MQ=255)

                               gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAAtttt                                                                                         >  1:760487/1‑139 (MQ=255)

                                     cgccgcTCAGTCATCGTCGCTGGCCGGGCGAAGCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTg                                                                                   <  2:687803/139‑1 (MQ=255)

                                           tCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                             <  2:791309/139‑1 (MQ=255)

                                                            ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                            <  2:760487/139‑1 (MQ=255)

                                                            ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                            <  2:359718/139‑1 (MQ=255)

                                                              gggCGAACCAGTGTCCGTTATCACTTGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAgg                                                          <  1:374368/139‑1 (MQ=255)

                                                                                                aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                        >  1:1581/1‑139 (MQ=255)

                                                                                                aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                        >  2:138043/1‑139 (MQ=255)

                                                                                                aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                        >  2:175854/1‑139 (MQ=255)

                                                                                                     gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                        >  1:30096/1‑86 (MQ=255)

                                                                                                     gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                        <  2:30096/86‑1 (MQ=255)

                                                                                                     gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccgg                   >  2:1883/1‑139 (MQ=255)

                                                                                                             gTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACa           <  1:737727/139‑1 (MQ=255)

                                                                                                                atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCAt                                                      <  2:81613/93‑1 (MQ=255)

                                                                                                                atgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCAt                                                      >  1:81613/1‑93 (MQ=255)

                                                                                                                 tgaATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg       >  2:398824/1‑139 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg  >  2:136134/1‑139 (MQ=255)

                                                                                                                      tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg  <  1:717611/139‑1 (MQ=255)

                                                                                                                           cAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                       >  2:253716/1‑113 (MQ=255)

                                                                                                                           cAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                       <  1:253716/113‑1 (MQ=255)

AACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCG  >  NC_000913/2200309‑2200566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A