Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 774 49.2 2112398 96.9% 2046913 118.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,200,454 (A)8→7 coding (176/3633 nt) yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,200,4470A.100.0% 126.9 / NA 31coding (169/3633 nt)yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/11);  total (20/11)

AACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTA  >  NC_000913/2200316‑2200576
                                                                                                                                   |                                                                                                                                 
aaCTGGAACTGAAATATAAAAAAGGCAAACCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaac                                                                                                                            <  1:1045122/139‑2 (MQ=255)
 aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaaca                                                                                                                           <  1:690947/139‑3 (MQ=255)
          gAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGc                                                                                                                 >  2:327517/1‑139 (MQ=255)
          gAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGc                                                                                                                 <  1:756604/139‑1 (MQ=255)
               ataAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTAt                                                                                                            >  1:251245/1‑139 (MQ=255)
                taAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATg                                                                                                           >  2:528348/1‑139 (MQ=255)
                    aaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGaa                                                                                                       <  2:526597/139‑1 (MQ=255)
                    aaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGaa                                                                                                       <  2:251245/139‑1 (MQ=255)
                       ggCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAAttt                                                                                                    >  1:299788/1‑139 (MQ=255)
                        gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAAtttt                                                                                                   >  2:539789/1‑139 (MQ=255)
                                    tCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTgg                                                                                       <  2:145803/139‑1 (MQ=255)
                                         cATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                  <  1:539789/139‑1 (MQ=255)
                                                  tGGCCGGGCGAACCAGTGTCCGTTATCCCTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATATTTCACTGGAGTGGCAGTCCGCCATCAc                                                                         >  2:355985/1‑139 (MQ=255)
                                                        ggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGc                                                                   >  2:481990/1‑139 (MQ=255)
                                                         gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGc                                                                                                                 >  2:144222/1‑92 (MQ=255)
                                                         gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGc                                                                                                                 <  1:144222/92‑1 (MQ=255)
                                                                   gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                                        <  1:528348/139‑1 (MQ=255)
                                                                            cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                               >  2:869395/1‑139 (MQ=255)
                                                                              cTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAacac                                             >  1:207554/1‑139 (MQ=255)
                                                                                      ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTc                                     >  2:571918/1‑139 (MQ=255)
                                                                                         aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                                  >  1:916360/1‑139 (MQ=255)
                                                                                           ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcc                                <  2:207554/139‑1 (MQ=255)
                                                                                              gACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccgg                             >  2:977762/1‑139 (MQ=255)
                                                                                               aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                            >  2:685402/1‑139 (MQ=255)
                                                                                                     ggTGATGAATTGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc                      >  2:682885/1‑139 (MQ=255)
                                                                                                         atgaATTGCGACAAAAAGCTGAGAAG‑AAAAAAACATTGCCTGGCGGATGGAGAATTTCTCACTGGAGTGGCAGTCCGCCATCACGACGGCCCACAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAAtt                  >  2:791459/1‑138 (MQ=255)
                                                                                                               tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg            <  1:869395/139‑1 (MQ=255)
                                                                                                               tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg            <  1:571918/139‑1 (MQ=255)
                                                                                                               tGCTACAAAAAGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg            <  2:730786/139‑1 (MQ=255)
                                                                                                                      aaaaGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCt     >  2:38460/1‑139 (MQ=255)
                                                                                                                      aaaaGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCt     >  2:310501/1‑139 (MQ=255)
                                                                                                                         aGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTa  >  2:172396/1‑139 (MQ=255)
                                                                                                                         aGCTGAGAAG‑AAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTa  >  1:192751/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                 
AACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAAACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTA  >  NC_000913/2200316‑2200576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A