Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,454,292	C→A	G122G (GGC→GGA)	paaA →	ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,454,292	0	C	A	100.0%	173.6 / NA	54	G122G (GGC→GGA)	paaA	ring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (30/24); total (30/24)

ATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCA  >  NC_000913/1454165‑1454425

ataGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                                                            <  1:893567/139‑1 (MQ=255)

     gccgccGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGgcg                                                                                                                       <  2:1310319/139‑1 (MQ=255)

      ccgccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGgcgc                                                                                                                      >  1:731259/1‑139 (MQ=255)

       cgccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCa                                                                                                                     >  2:1037687/1‑139 (MQ=255)

                   gggCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAAt                                                                                                         >  1:1175619/1‑139 (MQ=255)

                   gggCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAAt                                                                                                         <  2:1175619/139‑1 (MQ=255)

                     gCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCa                                                                                                       >  1:16637/1‑139 (MQ=255)

                         cgcCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTg                                                                                                   >  1:1229685/1‑139 (MQ=255)

                                  agaCATCTACCAAAAAATGCTCGACGGACGGATGAACTATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGc                                                                                          <  1:1037687/139‑1 (MQ=255)

                                       tCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                                                     <  2:89846/139‑1 (MQ=255)

                                           ccAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   >  2:816411/1‑105 (MQ=255)

                                           ccAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   <  1:816411/105‑1 (MQ=255)

                                              aaaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTAt                                                                              <  1:250477/139‑1 (MQ=255)

                                                aaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATgg                                                                            >  2:1491758/1‑139 (MQ=255)

                                                 aaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGc                                                                           <  2:16637/139‑1 (MQ=255)

                                                     cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGt                                                                       >  2:950198/1‑139 (MQ=255)

                                                                           cctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAAt                                                 >  1:458452/1‑139 (MQ=255)

                                                                              ccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTg                                              >  2:134437/1‑139 (MQ=255)

                                                                               cATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGc                                             >  1:1278715/1‑139 (MQ=255)

                                                                                 tCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        >  1:246926/1‑110 (MQ=255)

                                                                                 tCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        <  2:246926/110‑1 (MQ=255)

                                                                                 tCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCaa                                           >  2:803373/1‑139 (MQ=255)

                                                                                       aTTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     <  1:211442/139‑1 (MQ=255)

                                                                                           tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCtggt                                                                                                                               <  1:715521/45‑1 (MQ=255)

                                                                                           tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCtggt                                                                                                                               >  2:715521/1‑45 (MQ=255)

                                                                                           tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGt                                 >  1:870202/1‑139 (MQ=255)

                                                                                                  ttGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCAcc                          <  1:134437/139‑1 (MQ=255)

                                                                                                     aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAg                                                                                                                    <  2:1452217/46‑1 (MQ=255)

                                                                                                     aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAg                                                                                                                    >  1:1452217/1‑46 (MQ=255)

                                                                                                         gggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCa                   <  2:731259/139‑1 (MQ=255)

                                                                                                          ggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   <  1:1039585/42‑1 (MQ=255)

                                                                                                          ggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   >  2:1039585/1‑42 (MQ=255)

                                                                                                          ggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCAcc                          <  1:947343/131‑1 (MQ=255)

                                                                                                          ggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCAcc                          >  2:947343/1‑131 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAgg                                                                                                     >  1:1003156/1‑51 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAgg                                                                                                     <  2:1003156/51‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:1408142/75‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             >  2:1408142/1‑75 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGtt             >  2:829656/1‑139 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGtt             >  1:709215/1‑139 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGtt             >  1:1534095/1‑139 (MQ=255)

                                                                                                                     gtgtTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGc                                                                                       <  2:62472/59‑1 (MQ=255)

                                                                                                                     gtgtTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGc                                                                                       >  1:62472/1‑59 (MQ=255)

                                                                                                                         tATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             >  2:598607/1‑65 (MQ=255)

                                                                                                                         tATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:598607/65‑1 (MQ=255)

                                                                                                                         tATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCa                                            >  1:790969/1‑98 (MQ=255)

                                                                                                                         tATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCa                                            <  2:790969/98‑1 (MQ=255)

                                                                                                                         tATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAagaga                                   <  1:1220568/107‑1 (MQ=255)

                                                                                                                         tATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAagaga                                   >  2:1220568/1‑107 (MQ=255)

                                                                                                                          aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGaa         >  2:300786/1‑132 (MQ=255)

                                                                                                                          aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGaa         <  1:300786/132‑1 (MQ=255)

                                                                                                                          aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCa  >  2:1637664/1‑139 (MQ=255)

                                                                                                                           tCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGttt            <  1:1661044/128‑1 (MQ=255)

                                                                                                                           tCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGttt            >  2:1661044/1‑128 (MQ=255)

ATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCA  >  NC_000913/1454165‑1454425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A