Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,454,292	C→A	G122G (GGC→GGA)	paaA →	ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,454,292	0	C	A	100.0%	146.2 / NA	46	G122G (GGC→GGA)	paaA	ring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/24); total (22/24)

ATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGA  >  NC_000913/1454165‑1454390

ataGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                         <  2:511124/139‑1 (MQ=255)

    cgccgcCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGCTATCGGTGTTATCGTATGGCTGGTGGa                                                                                         >  1:1241586/1‑135 (MQ=255)

    cgccgcCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                         <  2:1241586/135‑1 (MQ=255)

       cgccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggatggc                                                                                                >  2:855036/1‑125 (MQ=255)

       cgccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggatggc                                                                                                <  1:855036/125‑1 (MQ=255)

                         cgcCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATc                                                                     <  1:499453/134‑1 (MQ=255)

                         cgcCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATc                                                                     >  2:499453/1‑134 (MQ=255)

                                                    gCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                     <  1:1225756/139‑1 (MQ=255)

                                                     cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCg                                                             <  1:266173/114‑1 (MQ=255)

                                                     cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCg                                                             >  2:266173/1‑114 (MQ=255)

                                                     cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGt                                    >  1:1415749/1‑139 (MQ=255)

                                                          cggacggaTGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAGTCAGGTGGCGTTATGCCGCACTTCTTATg                                          >  1:319634/1‑128 (MQ=255)

                                                          cggacggaTGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAGTCAGGTGGCGTTATGCCGCACTTCTTATg                                          <  2:319634/128‑1 (MQ=255)

                                                              cggaTGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCagag                           <  2:1415749/139‑1 (MQ=255)

                                                                           cctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTa                                                          <  2:781718/95‑1 (MQ=255)

                                                                           cctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTa                                                          >  1:781718/1‑95 (MQ=255)

                                                                                  cTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                >  1:665503/1‑66 (MQ=255)

                                                                                  cTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                <  2:665503/66‑1 (MQ=255)

                                                                                      aaTTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAAt                                                                      <  2:1048211/72‑1 (MQ=255)

                                                                                      aaTTATCCGACATTGAGTTGGGCTGATAGCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAAt                                                                      >  1:1048211/1‑72 (MQ=255)

                                                                                           tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                             <  1:1357271/60‑1 (MQ=255)

                                                                                           tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                             >  2:1357271/1‑60 (MQ=255)

                                                                                           tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAGCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                     >  1:1269266/1‑100 (MQ=255)

                                                                                           tCCGACATGGGGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                     <  2:1269266/100‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGa                                                                              >  1:1222506/1‑39 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGa                                                                              <  2:1222506/39‑1 (MQ=38)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATc                                                                            >  1:724946/1‑41 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATc                                                                            <  2:724946/41‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCaa                                                                       <  1:1083018/46‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCaa                                                                       >  2:1083018/1‑46 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                  >  2:373315/1‑67 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                  <  1:373315/67‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTa                                   <  2:411511/82‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTa                                   >  1:411511/1‑82 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCagag                           <  2:846549/90‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCagag                           >  1:846549/1‑90 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAAt                       <  2:1637750/94‑1 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAAt                       >  1:1637750/1‑94 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga  >  2:865706/1‑115 (MQ=255)

                                                                                                               atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga  <  1:865706/115‑1 (MQ=255)

                                                                                                                    ggTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                     >  1:1100975/1‑75 (MQ=255)

                                                                                                                    ggTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                     <  2:1100975/75‑1 (MQ=255)

                                                                                                                     gtgtTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTa                                   >  1:443713/1‑76 (MQ=255)

                                                                                                                     gtgtTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTa                                   <  2:443713/76‑1 (MQ=255)

                                                                                                                      tgtTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGc                                                       >  1:705857/1‑55 (MQ=255)

                                                                                                                      tgtTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGc                                                       <  2:705857/55‑1 (MQ=255)

                                                                                                                        ttATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCa         >  1:813700/1‑99 (MQ=255)

                                                                                                                        ttATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCa         <  2:813700/99‑1 (MQ=255)

ATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGA  >  NC_000913/1454165‑1454390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A