Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,454,292	C→A	G122G (GGC→GGA)	paaA →	ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,454,292	0	C	A	100.0%	153.8 / NA	48	G122G (GGC→GGA)	paaA	ring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (28/20); total (28/20)

TATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCA  >  NC_000913/1454158‑1454425

tATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggatggc                                                                                                                                   >  1:708123/1‑139 (MQ=255)

tATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCATTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggatggc                                                                                                                                   >  1:364627/1‑139 (MQ=255)

  tctcTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCATTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGgatggctg                                                                                                                                 <  2:364627/139‑1 (MQ=255)

       ataGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCTGATGGCTGGTGGa                                                                                                                            >  1:1007601/1‑139 (MQ=255)

       ataGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                                                            <  2:708123/139‑1 (MQ=255)

          gcgcCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATgg                                                                                                                         >  1:129432/1‑139 (MQ=255)

          gcgcCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATgg                                                                                                                         >  2:294481/1‑139 (MQ=255)

             ccgccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGgcgc                                                                                                                      >  2:148299/1‑139 (MQ=255)

              cgccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                                                            <  2:40507/132‑1 (MQ=255)

              cgccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGa                                                                                                                            >  1:40507/1‑132 (MQ=255)

                 cgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCg                                                                                                                  <  2:163588/139‑1 (MQ=255)

                           ggCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATc                                                                                                        >  1:193974/1‑139 (MQ=255)

                                cgcCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGTCATTGAGATGGGCTGATATCGTTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTg                                                                                                   >  2:123298/1‑139 (MQ=255)

                                cgcCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTg                                                                                                   >  2:177332/1‑139 (MQ=255)

                                 gcCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGCGTTGGGCTGATATGGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTgg                                                                                                  >  1:359483/1‑139 (MQ=255)

                                                      aaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:330732/139‑1 (MQ=255)

                                                      aaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:294481/139‑1 (MQ=255)

                                                            cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        <  1:418026/138‑1 (MQ=255)

                                                            cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        >  2:418026/1‑138 (MQ=255)

                                                            cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGt                                                                       >  2:18634/1‑139 (MQ=255)

                                                                                     ccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTg                                              >  1:892256/1‑139 (MQ=255)

                                                                                      cATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGc                                             >  1:411621/1‑139 (MQ=255)

                                                                                        tCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCa                                            >  1:721586/1‑138 (MQ=255)

                                                                                        tCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCa                                            <  2:721586/138‑1 (MQ=255)

                                                                                            tAATTATCCGACATTGAGTTGGGCTGATATCG‑‑GTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     <  1:585597/139‑1 (MQ=255)

                                                                                              aTTATCCGTCATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCACACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     <  2:193974/139‑1 (MQ=255)

                                                                                              aTTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     <  2:411621/139‑1 (MQ=255)

                                                                                                  tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        <  1:302714/100‑1 (MQ=255)

                                                                                                  tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        >  2:302714/1‑100 (MQ=255)

                                                                                                  tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGt                                 >  2:584786/1‑139 (MQ=255)

                                                                                                  tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGt                                 >  2:732893/1‑139 (MQ=255)

                                                                                                  tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGt                                 >  1:910647/1‑139 (MQ=255)

                                                                                                  tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGt                                 >  1:735935/1‑139 (MQ=255)

                                                                                                  tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGt                                 >  1:214645/1‑139 (MQ=255)

                                                                                                   ccGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGtt                                >  1:80370/1‑139 (MQ=255)

                                                                                                        aTTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCAc                           <  1:177332/139‑1 (MQ=255)

                                                                                                         ttGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCAcc                          <  2:214645/139‑1 (MQ=255)

                                                                                                         ttGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCAcc                          >  1:467826/1‑139 (MQ=255)

                                                                                                         ttGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCAcc                          <  2:892256/139‑1 (MQ=255)

                                                                                                                gggCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCTATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCa                   <  1:148299/139‑1 (MQ=255)

                                                                                                                  gCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAgg                 >  1:246838/1‑139 (MQ=255)

                                                                                                                      atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                                                                >  1:569042/1‑40 (MQ=255)

                                                                                                                      atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                                                                <  2:569042/40‑1 (MQ=255)

                                                                                                                      atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGtt             >  2:134315/1‑139 (MQ=255)

                                                                                                                      atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGtt             >  1:555158/1‑139 (MQ=255)

                                                                                                                          cGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGaa         <  1:107875/139‑1 (MQ=255)

                                                                                                                          cGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGaa         <  1:732893/139‑1 (MQ=255)

                                                                                                                             tgtTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGcc      <  1:134315/139‑1 (MQ=255)

                                                                                                                                tATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGaaa                                                   >  1:85197/1‑91 (MQ=255)

                                                                                                                                tATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGaaa                                                   <  2:85197/91‑1 (MQ=255)

                                                                                                                                 aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCa  >  2:431904/1‑139 (MQ=255)

                                                                                                                                 aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCa  >  2:104847/1‑139 (MQ=255)

TATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCA  >  NC_000913/1454158‑1454425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A