Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 770 68.5 3470224 91.7% 3182195 103.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,454,292 C→A G122G (GGC→GGA)  paaA → ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,454,2920CA100.0% 199.3 / NA 62G122G (GGC→GGA) paaAring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/31);  total (31/31)

TATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCA  >  NC_000913/1454164‑1454425
                                                                                                                                |                                                                                                                                     
tataGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggatgg                                                                                                                                    <  2:826424/132‑1 (MQ=255)
tataGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggatgg                                                                                                                                    >  1:826424/1‑132 (MQ=255)
  taGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGAt                                                                                                                           >  2:345294/1‑139 (MQ=255)
  taGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGAt                                                                                                                           >  2:873064/1‑139 (MQ=255)
  taGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGAt                                                                                                                           >  2:1627644/1‑139 (MQ=255)
    gcgcCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATgg                                                                                                                         >  2:1280910/1‑139 (MQ=255)
    gcgcCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATgg                                                                                                                         >  2:1633142/1‑139 (MQ=255)
      gccgccGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGgcg                                                                                                                       <  2:1605619/139‑1 (MQ=255)
          ccgAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   >  2:890450/1‑139 (MQ=255)
             aaaCACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGAt                                                                                                                <  2:1210734/139‑1 (MQ=255)
                        tGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATc                                                                                                        >  2:670277/1‑136 (MQ=255)
                        tGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATc                                                                                                        <  1:670277/136‑1 (MQ=255)
                                   agaCATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGc                                                                                          <  1:1633142/139‑1 (MQ=255)
                                    gaCATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAgg                                                                                                     <  2:402901/127‑1 (MQ=255)
                                    gaCATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAgg                                                                                                     >  1:402901/1‑127 (MQ=255)
                                        tCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                                                     <  1:890450/139‑1 (MQ=255)
                                         cTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGa                                                                                                                 >  2:1454548/1‑110 (MQ=255)
                                         cTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGa                                                                                                                 <  1:1454548/110‑1 (MQ=255)
                                         cTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAct                                                                                    >  1:1622654/1‑139 (MQ=255)
                                         cTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAct                                                                                    >  1:1605243/1‑139 (MQ=255)
                                               aaaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTAt                                                                              >  1:1358128/1‑139 (MQ=255)
                                                aaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:345294/139‑1 (MQ=255)
                                                aaaaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATg                                                                             <  1:873064/139‑1 (MQ=255)
                                                  aaTGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGGTATCGGATGGCTGGTTGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGc                                                                           <  2:1622654/139‑1 (MQ=255)
                                                     gCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        <  1:1627644/139‑1 (MQ=255)
                                                     gCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        <  1:1280910/139‑1 (MQ=255)
                                                      cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGgcg                                                                                                                       <  1:34266/91‑1 (MQ=255)
                                                      cTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGgcg                                                                                                                       >  2:34266/1‑91 (MQ=255)
                                                               cggaTGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCagag                                                              <  1:1206495/139‑1 (MQ=255)
                                                               cggaTGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCagag                                                              <  1:1421524/139‑1 (MQ=255)
                                                                ggaTGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGc                                                             <  2:1358128/139‑1 (MQ=255)
                                                                  aTGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCaa                                                           <  2:1605243/139‑1 (MQ=255)
                                                                           tcctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   >  1:659891/1‑74 (MQ=255)
                                                                           tcctccATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGc                                                                                                                   <  2:659891/74‑1 (MQ=255)
                                                                                cATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGc                                             >  1:637584/1‑139 (MQ=255)
                                                                                       aaTTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        >  1:1641094/1‑105 (MQ=255)
                                                                                       aaTTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCg                                                                        <  2:1641094/105‑1 (MQ=255)
                                                                                        aTTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     <  1:1275435/139‑1 (MQ=255)
                                                                                            tCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGt                                 >  2:743307/1‑139 (MQ=255)
                                                                                                aCATTGAGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTc                             >  2:1422893/1‑139 (MQ=255)
                                                                                                      aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGgcg                                                                                                                       >  1:221771/1‑43 (MQ=255)
                                                                                                      aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGgcg                                                                                                                       <  2:221771/43‑1 (MQ=255)
                                                                                                      aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGcc                                                                          <  2:1253791/88‑1 (MQ=255)
                                                                                                      aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGcc                                                                          >  1:1253791/1‑88 (MQ=255)
                                                                                                      aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCa                            >  1:597239/1‑134 (MQ=255)
                                                                                                      aGTTGGGCTGATATCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCa                            <  2:597239/134‑1 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATc                                                                                                               <  1:1606763/41‑1 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATc                                                                                                               >  2:1606763/1‑41 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTa                                                                                             <  1:382447/59‑1 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTa                                                                                             >  2:382447/1‑59 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCActtctt                                                                                >  1:478983/1‑72 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCActtctt                                                                                <  2:478983/72‑1 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                                                     >  1:585109/1‑67 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCAc                                                                                     <  2:585109/67‑1 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGc                                                                   >  1:632658/1‑85 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGc                                                                   <  2:632658/85‑1 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGtt             >  2:958749/1‑139 (MQ=255)
                                                                                                                atatCGGTGTTATCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGtt             >  1:1209073/1‑139 (MQ=255)
                                                                                                                           aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGc                                                                                       >  2:382433/1‑54 (MQ=255)
                                                                                                                           aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGc                                                                                       <  1:382433/54‑1 (MQ=255)
                                                                                                                           aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     <  2:24327/104‑1 (MQ=255)
                                                                                                                           aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAagaaga                                     >  1:24327/1‑104 (MQ=255)
                                                                                                                           aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCa  >  2:541222/1‑139 (MQ=255)
                                                                                                                           aTCGGATGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCa  <  1:743307/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                     
TATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAATCAGGTGGCGTTATGCCGCACTTCTTATGGCCCGTATGCCAGAGCAATGGTGAAAATCTGCAAAGAAGAGAGTTTTCACCAGCGTCAGGGGTTTGAAGCCTGCA  >  NC_000913/1454164‑1454425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A