Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,920,205 |
(T)7→8 |
intergenic (+62/‑18) |
yebT → / → rsmF |
MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,920,198 | 1 | . | T | 100.0%
| 74.4
/ NA
| 24 | intergenic (+55/‑25) | yebT/rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (15/9); total (15/9) |
GGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920068‑1920330
|
ggAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑tttttttt < 2:1035753/139‑2 (MQ=255)
cTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCt > 2:80952/1‑139 (MQ=255)
gTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCTCg < 1:80952/139‑1 (MQ=255)
aGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGccgtccggg > 2:750950/1‑131 (MQ=255)
gAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTTGCCAGTGGTAc < 2:588557/115‑1 (MQ=255)
gAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTTGCCAGTGGTAc > 1:588557/1‑115 (MQ=255)
gcgcTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGc > 1:512485/1‑127 (MQ=255)
gcgcTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGc < 2:512485/127‑1 (MQ=255)
aaaTAATGCCCACTGCTCCGGCGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgcgc > 1:634257/1‑139 (MQ=255)
atGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgcgc < 1:126140/132‑1 (MQ=255)
atGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgcgc > 2:126140/1‑132 (MQ=255)
gCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑ttttttttg > 2:233129/1‑61 (MQ=255)
gCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑ttttttttg < 1:233129/63‑3 (MQ=255)
tGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACAt > 2:1039031/1‑69 (MQ=255)
tGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACAt < 1:1039031/69‑1 (MQ=255)
tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg > 2:382566/1‑90 (MQ=255)
tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg < 1:382566/90‑1 (MQ=255)
cgcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGt < 1:864576/35‑1 (MQ=37)
cgcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGt > 2:864576/1‑35 (MQ=37)
cgcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCtt < 1:827752/97‑1 (MQ=255)
cgcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCtt > 2:827752/1‑97 (MQ=255)
cgcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 1:910522/1‑139 (MQ=255)
cgcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 2:920026/1‑139 (MQ=255)
gcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGa < 1:612357/99‑1 (MQ=255)
gcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGa > 2:612357/1‑99 (MQ=255)
gcgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGccc > 2:513012/1‑139 (MQ=255)
cgcCTGT‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCg > 1:829145/1‑139 (MQ=255)
|
GGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCG‑‑GCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920068‑1920330
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
