Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,920,205 (T)7→8 intergenic (+62/‑18) yebT → / → rsmF MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,920,1981.T95.5% 134.5 / ‑0.7 44intergenic (+55/‑25)yebT/rsmFMCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base T (23/19);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

AGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC  >  NC_000913/1920067‑1920328
                                                                                                                                    |                                                                                                                                  
aGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttt                                                                                                                             <  1:716379/139‑1 (MQ=255)
   aaGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc                                                                                                                          <  1:508358/139‑4 (MQ=255)
    aGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGGATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgcc                                                                                                                         <  1:868704/139‑5 (MQ=255)
    aGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGTCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgcc                                                                                                                         <  1:896761/139‑5 (MQ=255)
      gCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                        <  1:1614670/139‑1 (MQ=255)
                     tGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg                                                                                                         <  1:1547380/139‑1 (MQ=255)
                           aaaGTGAACCGCAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTTCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCaa                                                                                                   <  2:1172926/139‑1 (MQ=255)
                            aaGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAac                                                                                                  >  2:1034013/1‑139 (MQ=255)
                                  aCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca                                                                                               <  1:814220/136‑1 (MQ=255)
                                  aCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca                                                                                               >  2:814220/1‑136 (MQ=255)
                                        agagTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTAtt                                                                                      <  1:1034013/139‑1 (MQ=255)
                                            tGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTccc                                                                                  <  2:1045534/139‑1 (MQ=255)
                                                  gAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGc                                                                            >  1:1374649/1‑139 (MQ=255)
                                                   aaTGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacac                                                                                                <  2:449789/118‑1 (MQ=255)
                                                   aaTGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacac                                                                                                >  1:449789/1‑118 (MQ=255)
                                                      ggggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca                                                                                               <  2:1093648/116‑1 (MQ=255)
                                                      ggggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca                                                                                               >  1:1093648/1‑116 (MQ=255)
                                                        ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                        >  2:135136/1‑89 (MQ=255)
                                                        ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                                        <  1:135136/89‑1 (MQ=255)
                                                              gcgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGAcaca                                                                >  2:845806/1‑139 (MQ=255)
                                                               cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg                                                                                                           >  1:358428/1‑95 (MQ=255)
                                                               cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg                                                                                                           <  2:358428/95‑1 (MQ=255)
                                                               cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACAcc                                                                                             <  2:1171836/109‑1 (MQ=255)
                                                               cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACAcc                                                                                             >  1:1171836/1‑109 (MQ=255)
                                                               cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg                                                                             <  1:1413081/125‑1 (MQ=255)
                                                               cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg                                                                             >  2:1413081/1‑125 (MQ=255)
                                                               cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttttttt                                                                                                                            >  2:473855/1‑76 (MQ=255)
                                                               cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttttttt                                                                                                                            <  1:473855/77‑2 (MQ=255)
                                                                    cccAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgcg                                                          >  2:437666/1‑139 (MQ=255)
                                                                             tGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc                                                                       >  2:1249638/1‑117 (MQ=255)
                                                                             tGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc                                                                       <  1:1249638/117‑1 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACa                                                                                                                 >  1:403294/1‑74 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACa                                                                                                                 <  2:403294/74‑1 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac                                                                                              >  2:1314883/1‑93 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac                                                                                              <  1:1314883/93‑1 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca                                                                                               <  2:923293/92‑1 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca                                                                                               >  1:923293/1‑92 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg                                                >  1:390462/1‑139 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg                                                >  1:1277427/1‑139 (MQ=255)
                                                                              gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGc                                               >  1:1749069/1‑139 (MQ=255)
                                                                                  aCTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCtt                                            >  1:666542/1‑139 (MQ=255)
                                                                                   cTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg                                          <  2:1749069/139‑1 (MQ=255)
                                                                                    tGCTACGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg                                          >  1:1044403/1‑139 (MQ=255)
                                                                                     gCTCCGGGGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGa                                         <  1:1292026/139‑1 (MQ=255)
                                                                                                ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCgg                                                                                >  1:1381042/1‑89 (MQ=255)
                                                                                                ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTAAATGCTCGTGGCCCAACACACCGTTTATTTCCCgg                                                                                <  2:1381042/89‑1 (MQ=255)
                                                                                                          aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTc                    >  2:1120207/1‑139 (MQ=255)
                                                                                                                            cgcgcCTGTTTTTTTTGTCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGctct                                   <  2:1701018/106‑1 (MQ=255)
                                                                                                                            cgcgcCTGTTTTTTTTGTCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGctct                                   >  1:1701018/1‑106 (MQ=255)
                                                                                                                            cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc  >  1:491304/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                  
AGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC  >  NC_000913/1920067‑1920328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A