Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,920,205 |
(T)7→8 |
intergenic (+62/‑18) |
yebT → / → rsmF |
MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,920,198 | 1 | . | T | 100.0%
| 74.7
/ NA
| 24 | intergenic (+55/‑25) | yebT/rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (13/11); total (13/11) |
AGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC > NC_000913/1920091‑1920328
|
aGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGcc > 1:888554/1‑139 (MQ=255)
gAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac < 2:888554/139‑1 (MQ=255)
tGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg < 2:1083662/101‑1 (MQ=255)
tGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg > 1:1083662/1‑101 (MQ=255)
aaTGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGcc > 2:531576/1‑139 (MQ=255)
atGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac < 2:698708/95‑1 (MQ=255)
atGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac > 1:698708/1‑95 (MQ=255)
atGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGa < 1:531576/139‑1 (MQ=255)
tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg < 1:508640/104‑1 (MQ=255)
tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg > 2:508640/1‑104 (MQ=255)
tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc > 2:1329626/1‑55 (MQ=255)
tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc < 1:1329626/58‑4 (MQ=255)
tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttttttt > 1:500561/1‑55 (MQ=255)
tGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttttttt < 2:500561/56‑2 (MQ=255)
tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac < 1:146054/77‑1 (MQ=255)
tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac > 2:146054/1‑77 (MQ=255)
ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc < 1:839826/91‑1 (MQ=255)
ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc > 2:839826/1‑91 (MQ=255)
ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg > 2:119252/1‑121 (MQ=255)
ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg < 1:119252/121‑1 (MQ=255)
gAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg < 1:694470/83‑1 (MQ=255)
gAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg > 2:694470/1‑83 (MQ=255)
aaaCTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg < 1:73382/40‑1 (MQ=255)
aaaCTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg > 2:73382/1‑40 (MQ=38)
aaaCTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTc > 1:610/1‑139 (MQ=255)
cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTccc > 1:720643/1‑59 (MQ=255)
cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTccc < 2:720643/59‑1 (MQ=255)
cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 2:82654/1‑139 (MQ=255)
|
AGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCC > NC_000913/1920091‑1920328
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
