Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,920,205 |
(T)7→8 |
intergenic (+62/‑18) |
yebT → / → rsmF |
MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,920,198 | 1 | . | T | 93.9%
| 97.3
/ ‑0.1
| 33 | intergenic (+55/‑25) | yebT/rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (17/14); total (19/14) |
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 |
CAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑T‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920066‑1920330
|
cAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑ttttt > 2:293971/1‑139 (MQ=255)
aGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑tttttt > 2:934215/1‑139 (MQ=255)
ggCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑tttttttgcca < 1:919787/139‑6 (MQ=255)
gCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAg < 1:472659/139‑1 (MQ=255)
cacTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTa < 2:15234/139‑1 (MQ=255)
cTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACa < 1:29801/139‑1 (MQ=255)
ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGc > 2:326810/1‑139 (MQ=255)
ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGc > 2:874089/1‑139 (MQ=255)
ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑tttttt < 1:13419/123‑1 (MQ=255)
ccTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑tttttt > 2:13419/1‑123 (MQ=255)
tGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTg < 1:934215/139‑1 (MQ=255)
gAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCa > 2:146761/1‑139 (MQ=255)
aGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAaca < 1:146761/139‑1 (MQ=255)
aaCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca > 2:296255/1‑137 (MQ=255)
aaCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca < 1:296255/137‑1 (MQ=255)
tGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACGTGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg > 1:663385/1‑139 (MQ=255)
tGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg < 2:663385/139‑1 (MQ=255)
gAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGc < 1:25108/139‑1 (MQ=255)
ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑T‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTg > 2:733976/1‑139 (MQ=255)
cccAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgcg > 1:962781/1‑139 (MQ=255)
cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGc > 1:60999/1‑139 (MQ=255)
aCTGCTCCGGCGGGCCTTCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCtt < 2:141304/139‑1 (MQ=255)
tCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑ttttttt > 2:100460/1‑52 (MQ=255)
tCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑ttttttt < 1:100460/53‑2 (MQ=255)
cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACg < 2:1043608/137‑1 (MQ=255)
cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACg > 1:1043608/1‑137 (MQ=255)
cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGct > 2:759066/1‑139 (MQ=255)
cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGct > 2:972939/1‑139 (MQ=255)
ggCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGctc < 1:972939/139‑1 (MQ=255)
ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg < 1:769371/127‑1 (MQ=255)
ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg > 2:769371/1‑127 (MQ=255)
ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAttt > 2:1001859/1‑139 (MQ=255)
ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCATTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAttt > 2:753521/1‑139 (MQ=255)
tGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑ttttttttgc > 1:468815/1‑41 (MQ=255)
tGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑t‑ttttttttgc < 2:468815/45‑5 (MQ=255)
ccGGAGCGTTTATGCTAAACTGCGCGCCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTgatgat < 1:874089/139‑1 (MQ=255)
gAGCGTTTATGCTAAACTGCGCGCCTG‑t‑tttttttgc > 2:976306/1‑34 (MQ=255)
gAGCGTTTATGCTAAACTGCGCGCCTG‑t‑tttttttgc < 1:976306/37‑4 (MQ=255)
aGCGTTTATGCTAAACTGCGCGCCTG‑TCTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTc > 1:561900/1‑139 (MQ=255)
cgcgcCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTgccgcc < 2:132859/129‑1 (MQ=255)
cgcgcCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTgccgcc > 1:132859/1‑129 (MQ=255)
cgcgcCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 1:232413/1‑139 (MQ=255)
gcgcCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGccc > 1:535172/1‑139 (MQ=255)
cgcCTGTT‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCg > 1:594492/1‑139 (MQ=255)
|
CAGGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑T‑TTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920066‑1920330
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
