Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,484,897 (C)6→7 coding (524/3594 nt) evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,484,8911.C97.5% 286.0 / ‑1.5 81coding (518/3594 nt)evgShybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (38/41);  total (39/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG  >  NC_000913/2484758‑2485023
                                                                                                                                      |                                                                                                                                     
ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGAGTAGCAACTTA‑cc‑ccc                                                                                                                                 >  1:1418826/1‑139 (MQ=255)
     tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga                                                                                                                            <  2:1243522/139‑5 (MQ=255)
     tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga                                                                                                                            <  1:1348116/139‑5 (MQ=255)
       ttAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACg                                                                                                                           <  1:1239812/139‑1 (MQ=255)
           taatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGt                                                                                                                       <  2:217581/139‑1 (MQ=255)
            aatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTa                                                                                                                      <  1:298028/139‑1 (MQ=255)
                aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATtc                                                                                                                  >  2:1675150/1‑139 (MQ=255)
                aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATtc                                                                                                                  >  2:1739954/1‑139 (MQ=255)
                 cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATtca                                                                                                                 >  1:1698939/1‑139 (MQ=255)
                 cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCACCCCAGCCGAGGTAATtca                                                                                                                 >  2:230778/1‑139 (MQ=255)
                   ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATtgatc                                                                                                               >  2:1157130/1‑139 (MQ=255)
                    tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga                                                                                                                            <  1:452278/124‑5 (MQ=255)
                    tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga                                                                                                                            >  2:452278/1‑120 (MQ=255)
                           accaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTaa                                                                                                                     <  1:780292/125‑1 (MQ=255)
                           accaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTaa                                                                                                                     >  2:780292/1‑125 (MQ=255)
                                accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga                                                                                                                            <  1:1649451/112‑5 (MQ=255)
                                accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga                                                                                                                            >  2:1649451/1‑108 (MQ=255)
                                   aTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               <  1:1675150/139‑1 (MQ=255)
                                      gATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaac                                                                                            >  1:837107/1‑139 (MQ=255)
                                          aTTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt                                                                                        <  2:864485/139‑1 (MQ=255)
                                          aTTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAtt                                                                                        <  1:1739954/139‑1 (MQ=255)
                                            tACCTTTCCTGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCACACCAAAACCACTAAATATTGCTCGGGTAGCAAATTA‑CCTCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                      <  1:176501/139‑1 (MQ=255)
                                            tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                      <  2:118975/139‑1 (MQ=255)
                                                 ttCCGGCGCTTGTAAACACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt                                                                                 >  2:176501/1‑139 (MQ=255)
                                                 ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt                                                                                 >  2:247312/1‑139 (MQ=255)
                                                   ccGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGAAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtttt                                                                               >  2:717708/1‑139 (MQ=255)
                                                            gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCatcaa                                                                                                             >  2:956243/1‑100 (MQ=255)
                                                            gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCatcaa                                                                                                             <  1:956243/100‑1 (MQ=255)
                                                              aaCCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata                                                                    <  1:247312/139‑1 (MQ=255)
                                                                    ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTACTTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGc                                                              >  1:531766/1‑139 (MQ=255)
                                                                    ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGc                                                              >  2:970869/1‑139 (MQ=255)
                                                                      ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            <  2:837107/139‑1 (MQ=255)
                                                                       tCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                                  >  1:528212/1‑132 (MQ=255)
                                                                       tCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                                  <  2:528212/132‑1 (MQ=255)
                                                                           taccaactGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTAAATCAGGCATTAGCa                                                       <  1:717708/132‑1 (MQ=255)
                                                                           gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  1:1137668/139‑1 (MQ=255)
                                                                              tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                    <  1:812898/107‑1 (MQ=255)
                                                                              tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtct                                                                                    >  2:812898/1‑107 (MQ=255)
                                                                                  tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                    >  2:447525/1‑87 (MQ=255)
                                                                                  tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                    <  1:447525/87‑1 (MQ=255)
                                                                                     gACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                      <  2:1349662/98‑1 (MQ=255)
                                                                                     gACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                      >  1:1349662/1‑98 (MQ=255)
                                                                                       ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTTATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCTGCt                                           <  1:1157130/139‑1 (MQ=255)
                                                                                       ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           >  1:886818/1‑139 (MQ=255)
                                                                                       ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           >  2:1207328/1‑139 (MQ=255)
                                                                                          cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga                                                                                                                            >  2:1231844/1‑50 (MQ=255)
                                                                                          cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cccccaga                                                                                                                            <  1:1231844/54‑5 (MQ=255)
                                                                                           ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       <  1:1647135/139‑1 (MQ=255)
                                                                                            tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCaaa                                                                                                  >  1:1541895/1‑79 (MQ=255)
                                                                                            tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCaaa                                                                                                  <  2:1541895/79‑1 (MQ=255)
                                                                                            tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata                                                                    <  1:1574940/109‑1 (MQ=255)
                                                                                            tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTtata                                                                    >  2:1574940/1‑109 (MQ=255)
                                                                                            tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cc                                                                                                                                  <  1:1528078/46‑1 (MQ=255)
                                                                                            tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cc‑cc                                                                                                                                  >  2:1528078/1‑46 (MQ=255)
                                                                                                  aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTaa                                                                                                                     <  1:573503/54‑1 (MQ=255)
                                                                                                  aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTaa                                                                                                                     >  2:573503/1‑54 (MQ=255)
                                                                                                  aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                >  1:1762478/1‑139 (MQ=255)
                                                                                                   ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAg                                             <  1:306652/125‑1 (MQ=255)
                                                                                                   ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAg                                             >  2:306652/1‑125 (MQ=255)
                                                                                                   ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  2:60033/139‑1 (MQ=255)
                                                                                                   ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  2:260191/139‑1 (MQ=255)
                                                                                                      aaaCCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTAc                            <  2:531766/139‑1 (MQ=255)
                                                                                                       aaCCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTgg                                         >  1:1227329/1‑125 (MQ=255)
                                                                                                       aaCCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTgg                                         <  2:1227329/125‑1 (MQ=255)
                                                                                                       aaCCAGTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACt                           >  2:958512/1‑139 (MQ=255)
                                                                                                            gTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       >  1:83711/1‑122 (MQ=255)
                                                                                                            gTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       <  2:83711/122‑1 (MQ=255)
                                                                                                            gTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  2:5267/130‑1 (MQ=255)
                                                                                                            gTAAATATTGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               >  1:5267/1‑130 (MQ=255)
                                                                                                                   ttGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta               <  2:686133/139‑1 (MQ=255)
                                                                                                                    tGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt                                                                                <  2:579842/73‑1 (MQ=255)
                                                                                                                    tGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt                                                                                >  1:579842/1‑73 (MQ=255)
                                                                                                                    tGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATTTCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTaa              >  1:592975/1‑139 (MQ=255)
                                                                                                                    tGCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTaa              >  1:1441146/1‑139 (MQ=255)
                                                                                                                     gCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                >  1:1083876/1‑120 (MQ=255)
                                                                                                                     gCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                <  2:1083876/120‑1 (MQ=255)
                                                                                                                     gCTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAc             >  2:581822/1‑139 (MQ=255)
                                                                                                                      cTCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  1:1207328/139‑1 (MQ=255)
                                                                                                                       tCGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat           >  1:1788868/1‑139 (MQ=255)
                                                                                                                        cGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcatc          <  1:958512/139‑1 (MQ=255)
                                                                                                                        cGGGTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcatc          <  2:1305201/139‑1 (MQ=255)
                                                                                                                           gTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt                      >  1:615884/1‑124 (MQ=255)
                                                                                                                           gTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt                      <  2:615884/124‑1 (MQ=255)
                                                                                                                           gTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            >  2:1786448/1‑134 (MQ=255)
                                                                                                                           gTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  1:1786448/134‑1 (MQ=255)
                                                                                                                           gTAGCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCAtt       >  1:1208822/1‑139 (MQ=255)
                                                                                                                              gCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATTTCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc    <  2:592975/139‑1 (MQ=255)
                                                                                                                              gCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca                                      >  1:1632145/1‑105 (MQ=255)
                                                                                                                              gCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca                                      <  2:1632145/105‑1 (MQ=255)
                                                                                                                              gCAAATTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc    >  2:1314503/1‑139 (MQ=255)
                                                                                                                                aaaTTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag  <  1:970869/139‑1 (MQ=255)
                                                                                                                                aaaTTACCC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCAACCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag  <  2:1698939/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CC‑CCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG  >  NC_000913/2484758‑2485023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A