Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,897	(C)_6→7	coding (524/3594 nt)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,891	1	.	C	97.9%	328.6 / ‑0.9	96	coding (518/3594 nt)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (48/46); total (48/48)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

Only 100 of 118 total aligned reads displayed.

CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG  >  NC_000913/2484753‑2485023

cTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        <  1:258689/139‑1 (MQ=255)

     ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑c                                                                                                                                 >  2:439476/1‑139 (MQ=255)

                taatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGt                                                                                                                       <  2:890576/139‑1 (MQ=255)

                taatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGt                                                                                                                       <  2:351612/139‑1 (MQ=255)

                   tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAAt                                                                                                                    >  1:1180032/1‑139 (MQ=255)

                      cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATtca                                                                                                                 >  2:1058299/1‑139 (MQ=255)

                      cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑c                                                                                                                                 <  2:764212/122‑1 (MQ=255)

                      cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑c                                                                                                                                 >  1:764212/1‑122 (MQ=255)

                           ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTaa                                                                                                                     <  1:1035659/130‑1 (MQ=255)

                           ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTaa                                                                                                                     >  2:1035659/1‑130 (MQ=255)

                            tgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCC‑CCAGACGAGGTAATTCatcaatca                                                                                                          <  2:1136380/139‑1 (MQ=255)

                              cAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCAt                                                                                                         >  1:385379/1‑139 (MQ=255)

                                   aaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                    <  2:1247316/139‑1 (MQ=255)

                                     accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTaa                                                                                                                     >  2:421011/1‑120 (MQ=255)

                                     accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑cccaga                                                                                                                            >  1:1005584/1‑108 (MQ=255)

                                     accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑ccc                                                                                                                               >  1:1331642/1‑108 (MQ=255)

                                       caTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                                <  2:862918/139‑1 (MQ=255)

                                                ttACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCGCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa                                                                                       >  1:1474457/1‑139 (MQ=255)

                                                 tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               <  1:1434353/130‑1 (MQ=255)

                                                 tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               >  2:1434353/1‑130 (MQ=255)

                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTaa                                                                                                                     <  2:1629580/103‑1 (MQ=255)

                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTaa                                                                                                                     >  1:1629580/1‑103 (MQ=255)

                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt                                                                                 >  2:90968/1‑139 (MQ=255)

                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c                                                                                                                                      <  2:1863529/86‑1 (MQ=255)

                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c                                                                                                                                      >  1:1863529/1‑86 (MQ=255)

                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        <  1:303873/84‑1 (MQ=255)

                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        >  2:303873/1‑84 (MQ=255)

                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt                                                                                >  2:130356/1‑139 (MQ=255)

                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑cccaga                                                                                                                            <  2:1468520/94‑5 (MQ=255)

                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑cccaga                                                                                                                            <  2:1523010/94‑5 (MQ=255)

                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑cccaga                                                                                                                            >  1:1523010/1‑90 (MQ=255)

                                                        ccGGCGCTGGTAACCACCCTTCACGACTGAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCCCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtttt                                                                               >  2:1275776/1‑139 (MQ=255)

                                                                 gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               >  1:1720136/1‑114 (MQ=255)

                                                                 gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               <  2:1720136/114‑1 (MQ=255)

                                                                    accaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                   <  1:2007943/139‑1 (MQ=255)

                                                                      caccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCa                                                                 <  2:1077462/139‑1 (MQ=255)

                                                                           ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            <  1:90968/139‑1 (MQ=255)

                                                                           ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            >  1:147820/1‑139 (MQ=255)

                                                                                     aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCaaa                                                                                                  <  1:240472/91‑1 (MQ=255)

                                                                                     aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCaaa                                                                                                  >  2:240472/1‑91 (MQ=255)

                                                                                     aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt                                                  <  1:1275776/139‑1 (MQ=255)

                                                                                     aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt                                                  >  1:154663/1‑139 (MQ=255)

                                                                                      aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtc                                                 >  1:1293646/1‑139 (MQ=255)

                                                                                       tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        <  2:1411121/52‑1 (MQ=255)

                                                                                       tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        >  1:1411121/1‑52 (MQ=255)

                                                                                       tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                                >  1:391052/1‑139 (MQ=255)

                                                                                          gACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAg                                             <  1:1481297/139‑1 (MQ=255)

                                                                                            ccGCCTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           <  2:1400388/139‑1 (MQ=255)

                                                                                               cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           <  1:665212/136‑1 (MQ=255)

                                                                                               cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           >  2:665212/1‑136 (MQ=255)

                                                                                               cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTAATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAACTggg                                        <  1:130356/139‑1 (MQ=255)

                                                                                                 tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            >  2:234049/1‑117 (MQ=255)

                                                                                                 tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            <  1:234049/117‑1 (MQ=255)

                                                                                                  aCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac                                     <  2:391052/139‑1 (MQ=255)

                                                                                                   ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                    >  1:709583/1‑139 (MQ=255)

                                                                                                   ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGACGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                    <  1:1058299/139‑1 (MQ=255)

                                                                                                      cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATg                                 <  2:1474457/139‑1 (MQ=255)

                                                                                                      cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATg                                 >  1:1907893/1‑139 (MQ=255)

                                                                                                      cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑cccc‑cccaga                                                                                                                            >  2:1212974/1‑43 (MQ=255)

                                                                                                       aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                >  1:1386409/1‑139 (MQ=255)

                                                                                                       aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                >  2:1099024/1‑139 (MQ=255)

                                                                                                       aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                >  1:911807/1‑139 (MQ=255)

                                                                                                        ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              >  2:1569443/1‑92 (MQ=255)

                                                                                                        ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  2:1386409/139‑1 (MQ=255)

                                                                                                        ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  1:439476/139‑1 (MQ=255)

                                                                                                        ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  1:636995/139‑1 (MQ=255)

                                                                                                         cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCACCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              >  2:1934178/1‑91 (MQ=255)

                                                                                                         cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCACCAATCATTTCCAAAAGCAACAATGATCTCTTTTa                                                                              <  1:1934178/91‑1 (MQ=255)

                                                                                                         cAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c                                                                                                                                      <  2:815694/35‑1 (MQ=255)

                                                                                                         cAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c                                                                                                                                      >  1:815694/1‑35 (MQ=255)

                                                                                                              ccAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                                <  2:438408/116‑1 (MQ=255)

                                                                                                              ccAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                                >  1:438408/1‑116 (MQ=255)

                                                                                                               cAGTAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTa                        <  2:147820/139‑1 (MQ=255)

                                                                                                                  tAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       >  1:344726/1‑105 (MQ=255)

                                                                                                                  tAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  2:344726/105‑1 (MQ=255)

                                                                                                                  tAAATATTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTg                     >  2:1536526/1‑139 (MQ=255)

                                                                                                                      tatTGCTCGGGTAGCAAATTA‑CCCCGCCAGACGAGGTAATTCATCAATCAGTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtag                 <  1:1536526/139‑1 (MQ=255)

                                                                                                                       atTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTaa                                                                                                                     <  2:1399754/38‑1 (MQ=255)

                                                                                                                       atTGCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagt                <  2:1293646/139‑1 (MQ=255)

                                                                                                                          gCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       >  1:1789827/1‑113 (MQ=255)

                                                                                                                          gCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       <  2:1789827/113‑1 (MQ=255)

                                                                                                                          gCTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAc             >  1:1434418/1‑139 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              >  1:168584/1‑73 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg                                                               >  1:1142159/1‑88 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg                                                               <  2:554696/88‑1 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg                                                               >  1:554696/1‑88 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg                                                               <  2:1142159/88‑1 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            <  1:143083/91‑1 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGTTAGTAAct            >  2:1476601/1‑138 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            >  2:1980562/1‑139 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  2:188459/139‑1 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  2:154663/139‑1 (MQ=255)

                                                                                                                           cTCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            >  1:1115078/1‑139 (MQ=255)

                                                                                                                            tCGGGTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat           >  2:1265424/1‑139 (MQ=255)

                                                                                                                                gTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              <  1:1435834/68‑1 (MQ=255)

                                                                                                                                gTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              >  2:1435834/1‑68 (MQ=255)

                                                                                                                                gTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg                    <  2:1384686/126‑1 (MQ=255)

                                                                                                                                gTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg                    >  1:1384686/1‑126 (MQ=255)

                                                                                                                                gTAGCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta               >  1:954881/1‑131 (MQ=255)

                                                                                                                                   gCAAATTACCCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc    <  1:1980562/139‑1 (MQ=255)

CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑CCCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG  >  NC_000913/2484753‑2485023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A