Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,897	(C)_6→7	coding (524/3594 nt)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,891	1	.	C	97.3%	252.5 / ‑1.0	73	coding (518/3594 nt)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (35/36); total (35/38)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑C‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC  >  NC_000913/2484754‑2485021

ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c                                                                                                                                     <  1:1090917/139‑1 (MQ=255)

ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c                                                                                                                                     >  2:285184/1‑139 (MQ=255)

ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c                                                                                                                                     <  2:1301058/139‑1 (MQ=255)

ttCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c                                                                                                                                     <  1:197725/139‑1 (MQ=255)

  cgccgcCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑cc                                                                                                                                  >  2:1291803/1‑139 (MQ=255)

    ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑c                                                                                                                               >  2:584217/1‑139 (MQ=255)

         tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccaga                                                                                                                          <  2:787797/139‑5 (MQ=255)

         tctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccaga                                                                                                                          <  1:584217/139‑5 (MQ=255)

          ctTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccagac                                                                                                                         >  2:695817/1‑134 (MQ=255)

           ttAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACg                                                                                                                         <  1:159238/139‑1 (MQ=255)

                 atGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTaa                                                                                                                   <  1:1291803/139‑1 (MQ=255)

                   gACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAAtt                                                                                                                 >  2:679687/1‑139 (MQ=255)

                    aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATtc                                                                                                                >  2:696517/1‑139 (MQ=255)

                    aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑c                                                                                                                               >  1:275866/1‑123 (MQ=255)

                    aCATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑c                                                                                                                               <  2:275866/123‑1 (MQ=255)

                     cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTC‑GGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATtcat                                                                                                              >  2:528998/1‑139 (MQ=255)

                       ttGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATtcatc                                                                                                             >  1:1257723/1‑139 (MQ=255)

                        tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑c                                                                                                                               <  1:297684/119‑1 (MQ=255)

                        tgctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑c                                                                                                                               >  2:297684/1‑119 (MQ=255)

                          ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCatcaat                                                                                                          >  2:299683/1‑139 (MQ=255)

                               accaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCAttt                                                                                                     >  2:192373/1‑139 (MQ=255)

                                        ttGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccaga                                                                                                                          <  2:705012/108‑5 (MQ=255)

                                        ttGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccaga                                                                                                                          >  1:705012/1‑104 (MQ=255)

                                         tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa                                                                                           >  2:385958/1‑139 (MQ=255)

                                         tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa                                                                                           <  2:1257723/139‑1 (MQ=255)

                                         tGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTCACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGcaa                                                                                           <  1:696517/139‑1 (MQ=255)

                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                    >  2:1130706/1‑139 (MQ=255)

                                                tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑C‑CCCTCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAt                                                                                    <  2:929675/139‑1 (MQ=255)

                                                     ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt                                                                               >  2:313665/1‑139 (MQ=255)

                                                      tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt                                                                              >  2:372166/1‑139 (MQ=255)

                                                        cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  1:372166/139‑1 (MQ=255)

                                                        cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  1:299683/139‑1 (MQ=255)

                                                        cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  1:385958/139‑1 (MQ=255)

                                                        cGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCCCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  1:285184/139‑1 (MQ=255)

                                                          gcgcTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑ccca                                                                                                                            >  1:1137236/1‑86 (MQ=255)

                                                          gcgcTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑ccca                                                                                                                            <  2:1137236/88‑3 (MQ=255)

                                                                    ccaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                                <  1:695817/139‑1 (MQ=255)

                                                                    ccaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATc                                                                <  2:321009/139‑1 (MQ=255)

                                                                      accCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCatcaa                                                                                                           >  2:708943/1‑94 (MQ=255)

                                                                      accCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGCGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCatcaa                                                                                                           <  1:708943/94‑1 (MQ=255)

                                                                        ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                 <  1:75894/134‑1 (MQ=255)

                                                                        ccTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                 >  2:75894/1‑134 (MQ=255)

                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                          >  2:1110813/1‑139 (MQ=255)

                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                          >  2:721114/1‑139 (MQ=255)

                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                          >  2:154578/1‑139 (MQ=255)

                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                          >  1:314919/1‑139 (MQ=255)

                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccaga                                                                                                                          >  2:164740/1‑70 (MQ=255)

                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccaga                                                                                                                          <  1:164740/74‑5 (MQ=255)

                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑c                                                                                                                               <  2:45814/69‑1 (MQ=255)

                                                                          ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑c                                                                                                                               >  1:45814/1‑69 (MQ=255)

                                                                               gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                     <  1:172668/139‑1 (MQ=255)

                                                                               gACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                     <  2:486664/139‑1 (MQ=255)

                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTGAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc                                                  >  2:780543/1‑139 (MQ=255)

                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                             >  1:327498/1‑96 (MQ=255)

                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                             <  2:327498/96‑1 (MQ=255)

                                                                                  tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATcc                                                  >  2:1081357/1‑139 (MQ=255)

                                                                                    aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            <  2:1129408/111‑1 (MQ=255)

                                                                                    aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                            >  1:1129408/1‑111 (MQ=255)

                                                                                     aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccaga                                                                                                                          <  1:221315/63‑5 (MQ=255)

                                                                                     aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑c‑ccc‑cccaga                                                                                                                          >  2:221315/1‑59 (MQ=255)

                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                              >  1:946016/1‑139 (MQ=255)

                                                                                      tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                              >  2:351479/1‑139 (MQ=255)

                                                                                        cGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc                                                                                   <  2:1154939/100‑1 (MQ=255)

                                                                                        cGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc                                                                                   >  1:1154939/1‑100 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                       <  2:888858/47‑1 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                       >  1:888858/1‑47 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAgg                                                                                                                      >  2:413216/1‑62 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAgg                                                                                                                      <  1:413216/62‑1 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATTAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc                                                   <  2:772111/129‑1 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                 >  2:803352/1‑115 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                 <  1:803352/115‑1 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATc                                                   >  1:772111/1‑129 (MQ=255)

                                                                                           ccGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                         <  1:1130706/139‑1 (MQ=255)

                                                                                           ccGCTTACCTCACATAAACCAGTAAATATTGCTCTGGTTACAAATTA‑CACCC‑CCAAACTATCTAATTCATCAAGCTTTTCCAAGTGCAACAATTATCGCTTGTACAAATTTACATCAGGCATTAGCATCCGTTTCATCt                                         <  2:1244140/139‑1 (MQ=255)

                                                                                               ttACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                     <  2:1025255/139‑1 (MQ=255)

                                                                                                tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGca                                    >  1:1208738/1‑139 (MQ=255)

                                                                                                 aCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac                                   <  2:1208738/139‑1 (MQ=255)

                                                                                                  ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                  <  1:351479/139‑1 (MQ=255)

                                                                                                  ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                  <  1:192373/139‑1 (MQ=255)

                                                                                                    tCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAAt                                <  1:313665/139‑1 (MQ=255)

                                                                                                     cACCAAAACCAGTAAATATTGCTC‑GGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                              <  1:528998/139‑1 (MQ=255)

                                                                                                      aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                     >  1:1078401/1‑132 (MQ=255)

                                                                                                      aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                     <  2:1078401/132‑1 (MQ=255)

                                                                                                       ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                              >  2:60318/1‑74 (MQ=255)

                                                                                                       ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                              <  1:60318/74‑1 (MQ=255)

                                                                                                             ccAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                              >  2:1297642/1‑132 (MQ=255)

                                                                                                             ccAGTAAATATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                              <  1:1297642/132‑1 (MQ=255)

                                                                                                                   aaTATTGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgt                 <  1:780543/139‑1 (MQ=255)

                                                                                                                        tGCTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTaa            >  2:176866/1‑139 (MQ=255)

                                                                                                                          cTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                  >  1:945623/1‑115 (MQ=255)

                                                                                                                          cTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                  <  2:945623/115‑1 (MQ=255)

                                                                                                                          cTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca          <  2:314919/139‑1 (MQ=255)

                                                                                                                          cTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca          <  2:382946/139‑1 (MQ=255)

                                                                                                                          cTCGGGTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAACATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca          >  2:1027139/1‑139 (MQ=255)

                                                                                                                               gTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt                    >  1:19184/1‑124 (MQ=255)

                                                                                                                               gTAGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAtt                    <  2:19184/124‑1 (MQ=255)

                                                                                                                                 aGCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc   <  1:154578/139‑1 (MQ=255)

                                                                                                                                  gCAAATTACC‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc  >  1:705574/1‑139 (MQ=255)

TTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTA‑C‑CCC‑CCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACC  >  NC_000913/2484754‑2485021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A