Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I1 R1 502 34.8 1912488 98.2% 1878063 80.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,889,110 T→C K330K (AAA→AAG)  cysI ← sulfite reductase, hemoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,889,1100TC100.0% 168.0 / NA 46K330K (AAA→AAG) cysIsulfite reductase, hemoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

CCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGG  >  NC_000913/2889032‑2889186
                                                                              |                                                                            
ccAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAActt                                                                            <  1:521458/81‑1 (MQ=255)
 cAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAActt                                                                            >  2:521519/1‑80 (MQ=255)
  aGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGa                                                                          <  1:521459/81‑1 (MQ=255)
    ttATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATc                                                                        >  2:521512/1‑81 (MQ=255)
    ttATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATc                                                                        <  2:521460/81‑1 (MQ=255)
    ttATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGGCCGGGGAACTCATATGGACGGATCGGTTCAAACTTGATc                                                                        <  1:521461/81‑1 (MQ=255)
      atcatcAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATcc                                                                       <  1:521462/80‑1 (MQ=255)
       tcatcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATcccc                                                                     <  2:521463/81‑1 (MQ=255)
          tcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcg                                                                  >  1:521501/1‑81 (MQ=255)
            aaTGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcgcg                                                                <  2:521464/81‑1 (MQ=255)
            aaTGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcgc                                                                 >  1:521509/1‑80 (MQ=255)
            aaTGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcgc                                                                 >  1:521513/1‑80 (MQ=255)
                cccTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGc                                                            <  1:521465/81‑1 (MQ=255)
                 ccTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCt                                                           >  2:521508/1‑81 (MQ=255)
                    tAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCa                                                        >  1:521523/1‑81 (MQ=255)
                       cccAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACtt                                                     >  2:521537/1‑81 (MQ=255)
                         cAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTcc                                                   <  1:521466/81‑1 (MQ=255)
                         cAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTcc                                                   <  2:521467/81‑1 (MQ=255)
                          aGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCg                                                  >  1:521521/1‑81 (MQ=255)
                           gCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGc                                                 <  1:521468/81‑1 (MQ=255)
                             cAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCtt                                               <  2:521469/81‑1 (MQ=255)
                              aaTACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCtt                                               >  2:521522/1‑80 (MQ=255)
                               atgcGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTa                                             <  2:521471/78‑1 (MQ=255)
                               aTACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCttt                                              >  1:521526/1‑80 (MQ=255)
                               aTACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTa                                             <  1:521470/81‑1 (MQ=255)
                                   gATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACg                                         >  1:521511/1‑81 (MQ=255)
                                         cTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCaa                                   <  2:521472/81‑1 (MQ=255)
                                             tCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAcccc                               <  1:521473/81‑1 (MQ=255)
                                             tCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAcccc                               <  2:521474/81‑1 (MQ=255)
                                               cGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAccccc                              <  1:521475/80‑1 (MQ=255)
                                                ggTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAc                            <  1:521476/81‑1 (MQ=255)
                                                  tGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAcgc                          >  2:521514/1‑81 (MQ=255)
                                                    aaCTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAcgcg                         <  2:521477/80‑1 (MQ=255)
                                                     aCTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCt                       <  2:521478/81‑1 (MQ=255)
                                                         atatGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTcc                     <  2:521479/79‑1 (MQ=255)
                                                            tGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCgt                <  2:521480/81‑1 (MQ=255)
                                                             ggACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCgtg               >  2:521533/1‑81 (MQ=255)
                                                              gACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCgtgt              <  1:521481/81‑1 (MQ=255)
                                                               aCGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTa             >  1:521518/1‑81 (MQ=255)
                                                                cGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAt            <  2:521482/81‑1 (MQ=255)
                                                                 ggATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAtt           >  1:521517/1‑81 (MQ=255)
                                                                 ggATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAtt           >  1:521531/1‑81 (MQ=255)
                                                                     cGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGt       >  1:521530/1‑81 (MQ=255)
                                                                      ggTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGtt      >  1:521534/1‑81 (MQ=255)
                                                                          cAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTgg  >  1:521528/1‑81 (MQ=255)
                                                                          cAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTgg  >  1:521538/1‑81 (MQ=255)
                                                                          cAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTgg  >  1:521525/1‑81 (MQ=255)
                                                                          cAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTgg  >  2:521527/1‑81 (MQ=255)
                                                                              |                                                                            
CCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTGG  >  NC_000913/2889032‑2889186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A