Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 730 35.1 2107516 91.4% 1926269 80.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,889,110 T→C K330K (AAA→AAG)  cysI ← sulfite reductase, hemoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,889,1100TC100.0% 168.0 / NA 48K330K (AAA→AAG) cysIsulfite reductase, hemoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/22);  total (26/22)

ATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTG  >  NC_000913/2889038‑2889185
                                                                        |                                                                           
atcatcAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATccc                                                                     >  2:524183/1‑81 (MQ=255)
    tcaATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcg                                                                 <  1:524116/81‑1 (MQ=255)
     caATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcgc                                                                >  1:524178/1‑81 (MQ=255)
      aaTGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcgc                                                                <  2:524117/80‑1 (MQ=255)
      aaTGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCcgcgc                                                                >  1:524190/1‑80 (MQ=255)
           ccTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACATGATCCCCGCGCGACGCt                                                          <  2:524118/81‑1 (MQ=255)
            cTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGTACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTc                                                         >  2:524171/1‑81 (MQ=255)
            cTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTc                                                         >  2:524188/1‑81 (MQ=255)
            cTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTc                                                         >  1:524173/1‑81 (MQ=255)
              tAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCa                                                       >  2:524180/1‑81 (MQ=255)
               aaCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTcc                                                        <  2:524119/79‑1 (MQ=255)
                aCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCAc                                                      <  1:524120/80‑1 (MQ=255)
                aCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACt                                                     <  1:524121/81‑1 (MQ=255)
                  ccAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTc                                                   <  2:524122/81‑1 (MQ=255)
                    aGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCg                                                 <  1:524123/81‑1 (MQ=255)
                       cAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCtt                                              <  1:524124/81‑1 (MQ=255)
                        aaTACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCttt                                             <  2:524126/81‑1 (MQ=255)
                        aaTACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCttt                                             <  1:524125/81‑1 (MQ=255)
                        aaTACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCtt                                              >  1:524185/1‑80 (MQ=255)
                          tACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTaa                                           <  1:524127/81‑1 (MQ=255)
                            cGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTaaa                                          <  2:524128/80‑1 (MQ=255)
                              aTCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGt                                       >  2:524191/1‑81 (MQ=255)
                                 gCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGtctc                                    <  2:524130/81‑1 (MQ=255)
                                 gCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGtct                                     >  1:524205/1‑80 (MQ=255)
                                 gCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGtct                                     <  1:524129/80‑1 (MQ=255)
                                 gCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGtct                                     >  2:524182/1‑80 (MQ=255)
                                      gTCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAccc                               >  2:524175/1‑81 (MQ=255)
                                       tCCGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAcccc                              <  2:524131/81‑1 (MQ=255)
                                        ccGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAAcccc                              >  1:524181/1‑80 (MQ=255)
                                         cGGTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCa                            >  1:524176/1‑81 (MQ=255)
                                          ggTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAc                           <  2:524132/81‑1 (MQ=255)
                                          ggTGAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAc                           >  1:524189/1‑81 (MQ=255)
                                             gAACTCATATGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCAcgcg                        >  1:524209/1‑81 (MQ=255)
                                                    tatGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGc                 <  2:524133/81‑1 (MQ=255)
                                                     atGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCg                >  2:524177/1‑81 (MQ=255)
                                                     atGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCg                >  2:524186/1‑81 (MQ=255)
                                                      tGGACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCgt               >  1:524201/1‑81 (MQ=255)
                                                       ggACGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCgtg              >  2:524198/1‑81 (MQ=255)
                                                         aCGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTa            <  1:524134/81‑1 (MQ=255)
                                                          cGGATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAt           >  2:524158/1‑81 (MQ=255)
                                                           ggATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAtt          <  2:524135/81‑1 (MQ=255)
                                                            gATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAttt         >  1:524193/1‑81 (MQ=255)
                                                            gATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAttt         >  1:524192/1‑81 (MQ=255)
                                                            gATCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTAttt         <  1:524136/81‑1 (MQ=255)
                                                             aTCGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTg        <  2:524137/81‑1 (MQ=255)
                                                               cGGTTCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGt      >  2:524204/1‑81 (MQ=255)
                                                                   tCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGttttt  >  2:524207/1‑80 (MQ=255)
                                                                   tCAAACTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTg  >  1:524187/1‑81 (MQ=255)
                                                                        |                                                                           
ATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACGTCTCAACCCCCACGCGCTCCAGCGTGTATTTGGTTTTG  >  NC_000913/2889038‑2889185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A