Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 730 35.1 2107516 91.4% 1926269 80.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,865,111 T→C I4T (ATT→ACT)  ygbN → putative transporter YgbN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,865,1110TC100.0% 111.1 / NA 32I4T (ATT→ACT) ygbNputative transporter YgbN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/20);  total (12/20)

AACGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTC  >  NC_000913/2865033‑2865187
                                                                              |                                                                            
aaCGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTa                                                                            >  1:519225/1‑81 (MQ=255)
  cGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACa                                                                          <  2:519197/81‑1 (MQ=255)
   gTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACAt                                                                         >  1:519241/1‑81 (MQ=255)
    tACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACAtt                                                                        >  2:519238/1‑81 (MQ=255)
        cATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTa                                                                    <  2:519198/81‑1 (MQ=255)
          tACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATg                                                                  <  2:519199/81‑1 (MQ=255)
               ccccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTg                                                             >  1:519250/1‑81 (MQ=255)
                cccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGc                                                            <  1:519200/81‑1 (MQ=255)
                          cGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCt                                                     <  1:519201/78‑1 (MQ=255)
                             aTGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTa                                               <  2:519202/81‑1 (MQ=255)
                                gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATc                                            <  1:519203/81‑1 (MQ=255)
                                 gttAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCa                                           >  1:519258/3‑81 (MQ=255)
                                 ggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCa                                           >  1:519253/1‑81 (MQ=255)
                                   tAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATg                                         >  2:519252/1‑81 (MQ=255)
                                       ttAACTATAACTTTCAGACAGGGTTTCCCATATCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGt                                     <  2:519204/81‑1 (MQ=255)
                                         aaCTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGtt                                    <  2:519205/80‑1 (MQ=255)
                                          aCTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTtgc                                  <  1:519206/81‑1 (MQ=255)
                                                aCTTTCAGACAGGGTTTCCCATGTCTACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGctgctg                             <  2:519207/80‑1 (MQ=255)
                                                  tttCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGt                           >  1:519248/1‑80 (MQ=255)
                                                     cAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGtcat                        >  1:519256/1‑80 (MQ=255)
                                                      aGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGtcatca                      <  2:519208/81‑1 (MQ=255)
                                                      aGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGtcatc                       >  2:519254/1‑80 (MQ=255)
                                                       gACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCaa                     <  1:519209/81‑1 (MQ=255)
                                                          aGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAgg                   >  2:519249/1‑80 (MQ=255)
                                                           gggTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGc                  >  2:519245/1‑80 (MQ=255)
                                                           gggTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCa                 <  2:519210/81‑1 (MQ=255)
                                                                       tCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCa     <  1:519212/81‑1 (MQ=255)
                                                                       tCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCa     <  2:519211/81‑1 (MQ=255)
                                                                         cacaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCAtt   <  1:519213/81‑1 (MQ=255)
                                                                         cacaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCAt    <  1:519214/80‑1 (MQ=255)
                                                                         cacaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCAt    <  1:519215/80‑1 (MQ=255)
                                                                          acaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTc  <  1:519216/81‑1 (MQ=255)
                                                                              |                                                                            
AACGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTC  >  NC_000913/2865033‑2865187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A