Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,948,577 |
G→C |
A145G (GCC→GGC) |
amiC ← |
N‑acetylmuramoyl‑L‑alanine amidase C |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,948,577 | 0 | G | C | 100.0%
| 119.5
/ NA
| 35 | A145G (GCC→GGC) | amiC | N‑acetylmuramoyl‑L‑alanine amidase C |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (13/22); total (13/22) |
GGCACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTT > NC_000913/2948499‑2948654
|
ggcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGccc > 2:536832/1‑81 (MQ=255)
gcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCg > 2:536838/1‑81 (MQ=255)
gcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCg < 2:536793/81‑1 (MQ=255)
gcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCg < 2:536792/81‑1 (MQ=255)
gCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATa > 1:536844/1‑79 (MQ=255)
cTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATaga < 2:536794/80‑1 (MQ=255)
gTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCAc < 1:536795/81‑1 (MQ=255)
tCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCAcc < 2:536797/81‑1 (MQ=255)
tCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCAcc < 2:536796/81‑1 (MQ=255)
tCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCAc > 2:536848/1‑80 (MQ=255)
ttgttgTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGc > 1:536839/1‑81 (MQ=255)
tgttgtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGc < 1:536798/80‑1 (MQ=255)
cagcagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGc > 2:536846/1‑81 (MQ=255)
agcagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGc > 2:536842/1‑80 (MQ=255)
cagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGAc < 2:536799/81‑1 (MQ=255)
cgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCgg < 2:536800/81‑1 (MQ=255)
gccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgc > 1:536840/1‑81 (MQ=255)
gccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgc < 1:536801/81‑1 (MQ=255)
cagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgcgc < 1:536802/81‑1 (MQ=255)
agcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgcgc > 2:536841/1‑80 (MQ=255)
agcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCa < 2:536803/81‑1 (MQ=255)
gcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgcgc > 2:536852/1‑79 (MQ=255)
gcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCaa < 1:536804/81‑1 (MQ=255)
agcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAgg > 1:536843/1‑81 (MQ=255)
agcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAgg > 2:536849/1‑81 (MQ=255)
gggTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCa < 1:536806/81‑1 (MQ=255)
gggTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCa < 1:536805/81‑1 (MQ=255)
tCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAAc < 2:536807/81‑1 (MQ=255)
cATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCt < 1:536808/80‑1 (MQ=255)
cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGtttt > 2:536866/1‑81 (MQ=255)
cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGttt < 1:536810/81‑1 (MQ=255)
cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGttt < 1:536811/81‑1 (MQ=255)
cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGtt < 2:536809/80‑1 (MQ=255)
cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGtt < 1:536812/80‑1 (MQ=255)
gtgCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTa > 2:536856/1‑81 (MQ=255)
ttGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGttt < 1:536813/81‑1 (MQ=255)
|
GGCACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTT > NC_000913/2948499‑2948654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
