Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,948,577 |
G→C |
A145G (GCC→GGC) |
amiC ← |
N‑acetylmuramoyl‑L‑alanine amidase C |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,948,577 | 0 | G | C | 100.0%
| 122.6
/ NA
| 35 | A145G (GCC→GGC) | amiC | N‑acetylmuramoyl‑L‑alanine amidase C |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (11/24); total (11/24) |
GGCACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACG > NC_000913/2948499‑2948651
|
ggcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGccc > 1:481191/1‑81 (MQ=255)
gcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCg < 2:481134/81‑1 (MQ=255)
cACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCgg < 1:481135/81‑1 (MQ=255)
aCCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGa > 2:481189/1‑81 (MQ=255)
aCCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGa > 1:481179/1‑81 (MQ=255)
ccTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGAt < 2:481136/81‑1 (MQ=255)
cTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATa < 2:481137/81‑1 (MQ=255)
cTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATaga < 1:481138/80‑1 (MQ=255)
cGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTcc < 2:481140/80‑1 (MQ=255)
cGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTcc < 2:481139/80‑1 (MQ=255)
gAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCa < 2:481141/80‑1 (MQ=255)
aGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATc < 1:481142/81‑1 (MQ=255)
ggTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCa > 2:481192/1‑81 (MQ=255)
cTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGAc > 2:481194/1‑81 (MQ=255)
tgttgtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACg < 2:481144/79‑1 (MQ=255)
tgttgtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGc < 2:481143/80‑1 (MQ=255)
gtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCtt < 1:481145/80‑1 (MQ=255)
gtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCtt > 2:481190/1‑80 (MQ=255)
tAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCttt < 2:481146/80‑1 (MQ=255)
cagcagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCg < 2:481147/79‑1 (MQ=255)
cagcagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGc > 1:481203/1‑81 (MQ=255)
agcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGAcc < 1:481148/81‑1 (MQ=255)
agcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGAcc > 1:481193/1‑81 (MQ=255)
cagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgcgc < 2:481149/81‑1 (MQ=255)
agcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAgg < 1:481150/81‑1 (MQ=255)
ggTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCa > 2:481188/1‑80 (MQ=255)
gTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCaaa > 1:481186/1‑81 (MQ=255)
cTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAg < 1:481151/81‑1 (MQ=255)
cTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAg > 1:481187/1‑81 (MQ=255)
tatCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCg < 1:481152/81‑1 (MQ=255)
atCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCgg > 2:481199/1‑81 (MQ=255)
tCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGt < 2:481154/81‑1 (MQ=255)
tCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGt < 2:481153/81‑1 (MQ=255)
tgCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGtttt < 2:481155/79‑1 (MQ=255)
tgCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGtttt < 2:481156/79‑1 (MQ=255)
cATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACg < 2:481157/80‑1 (MQ=255)
|
GGCACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACG > NC_000913/2948499‑2948651
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
