Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 182 31.9 1789978 98.5% 1763128 80.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,300,767 T→G V170V (GTA→GTC)  yhbT ← SCP2 domain‑containing protein YhbT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,300,7670TG100.0% 150.1 / NA 41V170V (GTA→GTC) yhbTSCP2 domain‑containing protein YhbT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/23);  total (18/23)

TCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCG  >  NC_000913/3300691‑3300844
                                                                            |                                                                             
tCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGAcc                                                                            >  1:570759/1‑80 (MQ=255)
 cGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCAccgaccga                                                                          >  2:570778/1‑81 (MQ=255)
  gAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTTAccgaccga                                                                          <  2:570706/80‑1 (MQ=255)
  gAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCAccgaccga                                                                          <  1:570705/80‑1 (MQ=255)
  gAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGAt                                                                         >  2:570764/1‑81 (MQ=255)
    aTGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGt                                                                       >  2:570755/1‑81 (MQ=255)
       aGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCt                                                                     <  2:570707/80‑1 (MQ=255)
         cGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGtt                                                                  >  2:570782/1‑81 (MQ=255)
              cagcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGtt                                                             <  2:570708/81‑1 (MQ=255)
              cagcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGtt                                                             >  1:570756/1‑81 (MQ=255)
                gcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGttt                                                            >  1:570773/1‑80 (MQ=255)
                gcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTc                                                           <  2:570709/81‑1 (MQ=255)
                 cagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCa                                                          <  1:570710/81‑1 (MQ=255)
                  agGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAg                                                         >  2:570777/1‑81 (MQ=255)
                    ggCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGgc                                                       <  1:570711/81‑1 (MQ=255)
                       aTCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGcgcg                                                    >  1:570762/1‑81 (MQ=255)
                           aTACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGttt                                                <  2:570712/81‑1 (MQ=255)
                                cGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCAtt                                           <  2:570713/81‑1 (MQ=255)
                                 ggCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTc                                          >  1:570770/1‑81 (MQ=255)
                                  gcgcATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTcc                                         <  1:570714/81‑1 (MQ=255)
                                     cATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGc                                      >  1:570765/1‑81 (MQ=255)
                                     cATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGc                                      >  1:570772/1‑81 (MQ=255)
                                      aTCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGcc                                     >  1:570781/1‑81 (MQ=255)
                                      aTCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGcc                                     <  1:570715/81‑1 (MQ=255)
                                       tCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCt                                    <  1:570716/81‑1 (MQ=255)
                                        cAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCt                                    >  1:570768/1‑80 (MQ=255)
                                         aaTGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTca                                  <  1:570717/81‑1 (MQ=255)
                                         aaTGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTc                                   <  2:570718/80‑1 (MQ=255)
                                          aTGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTcaa                                 >  1:570771/1‑81 (MQ=255)
                                             ggAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTcaaca                               <  2:570719/80‑1 (MQ=255)
                                               aaTTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACaaaa                            >  1:570780/1‑81 (MQ=255)
                                                       cTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGcc                      <  1:570721/79‑1 (MQ=255)
                                                       cTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGc                       <  1:570720/78‑1 (MQ=255)
                                                          gAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGtt                  <  2:570722/80‑1 (MQ=255)
                                                            aTTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgca               <  1:570724/81‑1 (MQ=255)
                                                            aTTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgca               <  1:570723/81‑1 (MQ=255)
                                                                gCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgcagca            <  1:570725/80‑1 (MQ=255)
                                                                 cATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgcagcag           >  2:570779/1‑80 (MQ=255)
                                                                   tGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcat        <  2:570726/81‑1 (MQ=255)
                                                                    ggTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcat        >  2:570788/1‑80 (MQ=255)
                                                                     gTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcatc       <  1:570729/80‑1 (MQ=255)
                                                                     gTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcatc       <  2:570728/80‑1 (MQ=255)
                                                                     gTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATT‑CCGCCTCAACAAAATCCGCCAGTTGCAGCAGcatca      <  2:570727/80‑1 (MQ=255)
                                                                       tCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATg    >  1:570784/1‑81 (MQ=255)
                                                                         accgaccgATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCg  >  1:570789/1‑81 (MQ=255)
                                                                            |                                                                             
TCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCG  >  NC_000913/3300691‑3300844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A