Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,493,804 |
T→C |
intergenic (+55/‑207) |
tsgA → / → nirB |
putative transporter TsgA/nitrite reductase catalytic subunit NirB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,493,804 | 0 | T | C | 100.0%
| 67.3
/ NA
| 20 | intergenic (+55/‑207) | tsgA/nirB | putative transporter TsgA/nitrite reductase catalytic subunit NirB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/14); total (6/14) |
CATAACACCCTGACCTCTCATTAATTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCTACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACAAACATGAAATATCAGACAAT > NC_000913/3493726‑3493873
|
cATAACACCCTGACCTCTCATTAATTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCAc < 2:602237/81‑1 (MQ=255)
tAACACCCTGACCTCTCATTAATTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGt > 2:602260/1‑81 (MQ=255)
aaCACCCTGACCTCTCATTAATTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTg > 2:602268/1‑81 (MQ=255)
ccTGACCTCTCATTAATTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCt < 1:602239/81‑1 (MQ=255)
aTTAATTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCtat < 2:602239/78‑1 (MQ=255)
aTTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAAccc < 1:602241/80‑1 (MQ=255)
aTTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAAccc < 1:602242/80‑1 (MQ=255)
cATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCAtt > 2:602271/1‑81 (MQ=255)
gCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTg > 2:602261/1‑81 (MQ=255)
aCGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTAt < 2:602243/81‑1 (MQ=255)
aCGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTAt < 1:602243/81‑1 (MQ=255)
cACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTAttttt < 1:602245/81‑1 (MQ=255)
cgtcgtCCGGCCTTTTCCTCTCTTTCCCCGCCACGTGCATCTATTTCTATAAACTCGCTCATTTTGTCTATTTTTTGcac > 2:602265/1‑80 (MQ=255)
tcgtcCGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACaaa < 2:602245/81‑1 (MQ=255)
cGGCCTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACAAACATGa < 2:602246/81‑1 (MQ=255)
cTTTTCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACAAACATGAAata < 1:602248/81‑1 (MQ=255)
ttCCTCTCTTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACAAACATGAAatat < 1:602249/79‑1 (MQ=255)
ctctctTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACAAACATGAAATATCAGAc < 1:602250/81‑1 (MQ=255)
tctTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACAAACATGAAATATCAGACa < 1:602251/79‑1 (MQ=255)
tctTCCCCCGCCACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACAAACATGAAATATCAGACAAt > 1:602273/1‑81 (MQ=255)
|
CATAACACCCTGACCTCTCATTAATTGCTCATGCCGGACGGCACTATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCTACGTGCATCTATTTCTATAAACCCGCTCATTTTGTCTATTTTTTGCACAAACATGAAATATCAGACAAT > NC_000913/3493726‑3493873
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
