Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 16 48.9 1703346 91.6% 1560264 146.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,795,396 C→A Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,795,3960CA100.0% 160.4 / NA 48Q382K (CAG→AAG) gabPgamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/22);  total (26/22)

CGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACA  >  NC_000913_3_pae_tpiA/2795257‑2795535
                                                                                                                                           |                                                                                                                                            
cGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGa                                                                                                                                     >  1:70695/1‑149 (MQ=255)
 gTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGAt                                                                                                                                    <  1:50504/149‑1 (MQ=255)
   ggCGGTTTTACTTTCGTCCGTCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATgc                                                                                                                                  <  1:71266/149‑1 (MQ=255)
    gCGGTGTTACTCTCCACCGGCGAGGCATTCATAACGGGGAGCGTGAACTATTAAGCGCCTGCGAAGGTATTTAAATTTCTGATGGACAGCTCCGGCCCTATCGCCGTGCTGGTTTATTTAGTACTGGCGGTGTCAAAGTTGCGACTgcg                                                                                                                                 >  2:144463/1‑149 (MQ=255)
      ggTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCa                                                                                                                               <  1:188078/149‑1 (MQ=255)
              tctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTg                                                                                                                       >  1:747497/1‑149 (MQ=255)
              tctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTg                                                                                                                       >  1:636888/1‑149 (MQ=255)
                   aCCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGc                                                                                                                  >  1:609324/1‑149 (MQ=255)
                    ccGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCa                                                                                                                 <  1:468082/149‑1 (MQ=255)
                            gCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAgaaggaag                                                                                                         >  1:821913/1‑149 (MQ=255)
                                  ttAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAt                                                                                                   >  2:61434/1‑149 (MQ=255)
                                  ttAACGGTGGCGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGAAAAATTCTGCGAGCACAAGGAAGCGAAAt                                                                                                   >  2:318374/1‑149 (MQ=255)
                                    aaCGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCCTAGGAGTCGCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTc                                                                                                 <  1:803901/149‑1 (MQ=255)
                                               tgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAtg                                                                                      >  2:493817/1‑149 (MQ=255)
                                                 aaCTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAtgtg                                                                                    >  1:267032/1‑149 (MQ=255)
                                                  aCTATTACGGGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATTTgg                                                                                   <  2:528214/149‑1 (MQ=255)
                                                     aTTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt                                                                                >  1:400459/1‑149 (MQ=255)
                                                                  gAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCtaa                                                                   >  1:403431/1‑147 (MQ=255)
                                                                     ggTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCt                                                               <  2:728688/149‑1 (MQ=255)
                                                                     ggTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCt                                                               <  1:637062/149‑1 (MQ=255)
                                                                        aTTTAAAGTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGc                                                            <  1:720856/149‑1 (MQ=255)
                                                                             aaTTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTc                                                       >  1:808261/1‑149 (MQ=255)
                                                                                 tgcgATCGACAGCTCCGGCGCTCTCCCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCGGGCTAGTCATCg                                                   <  1:324313/146‑1 (MQ=255)
                                                                                 tCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCg                                                   <  2:614949/149‑1 (MQ=255)
                                                                                   tGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCCTCGGc                                                 >  1:33959/1‑149 (MQ=255)
                                                                                   tGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGc                                                 >  1:560301/1‑149 (MQ=255)
                                                                                     aTCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGAtt                                               >  1:349823/1‑149 (MQ=255)
                                                                                      tCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCttt                                              >  1:485469/1‑149 (MQ=255)
                                                                                      tCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTACGGATGCGCAAAATTATGCGAGCAGAAGCAAGCGAAATTCGCTTGCGAATGTGGCTTTGTCCGTGGCC‑CACCTGGCTAGTCTTCGGCttt                                              >  2:380074/1‑149 (MQ=255)
                                                                                         aCAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTttatt                                           >  2:90450/1‑149 (MQ=255)
                                                                                         aCAGCTCCGGCGCGATCGCCCTGCTGGCTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGACGCGAAATGCGCTTGCGAATGTGGCTCTATCCGTGGCT‑CACCTGGCTAGTCGTCGGCTttatt                                           >  2:472898/1‑149 (MQ=255)
                                                                                          cAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTttatta                                          >  1:397542/1‑149 (MQ=255)
                                                                                            ggcccGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTA‑CAGTTAATGCACCTGGCTAGTCATCGGCTTTATTAcc                                        <  2:822550/146‑1 (MQ=255)
                                                                                              tCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGc                                                                                                        >  1:22361/1‑84 (MQ=255)
                                                                                              tCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGc                                                                                                        <  2:22361/84‑1 (MQ=255)
                                                                                                   cgcTATCGCCCTGCTGGTTTATTTATTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTtgttt                                 <  2:529934/149‑3 (MQ=255)
                                                                                                     cTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTg                               <  2:560301/149‑1 (MQ=255)
                                                                                                        tCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTATATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTa                            <  1:242292/149‑1 (MQ=255)
                                                                                                            ccTGCTGGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGa                        <  2:267032/149‑1 (MQ=255)
                                                                                                              tgctgGTTTAGTTGGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATg                      <  1:29969/149‑1 (MQ=255)
                                                                                                              tgcagttttgtttaGTGATCGCGGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATg                      <  2:650342/143‑1 (MQ=255)
                                                                                                                ctgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCt                    >  1:729606/1‑149 (MQ=255)
                                                                                                                 tgGTTTATTTAGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTa                   >  1:530361/1‑149 (MQ=255)
                                                                                                                    tttatttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATtc                >  1:34587/1‑149 (MQ=255)
                                                                                                                      tatttaGTCATCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATTCTTTtccg              >  2:159531/1‑149 (MQ=255)
                                                                                                                                tggctgTTTCAAATTTGGCGATGCGCAAAATTCTTACAGCAGAAGGAAGCGAAATGCGCTTGCGAATGTGGCTTTATCCGTGCCT‑CACCTGGCTAGGCATCGGCGTTATTGCCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCaa    <  1:343005/144‑1 (MQ=255)
                                                                                                                                tCGCCGTTTCAAAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCaa    <  2:808261/149‑1 (MQ=255)
                                                                                                                                  gCCGTTTCAAAGTTGCGGATGCGCAAACTTCTGCGCGCAGGAAGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACa  <  1:232983/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCT‑CACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGTGATGCTATTCCGTCCGGCGCAACA  >  NC_000913_3_pae_tpiA/2795257‑2795535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A